Abstract. Increases in observed atmospheric concentrations of the long-lived greenhouse gas, nitrous oxide (N2O), have been well documented. However, information on event-related instantaneous emissions during fertilizer applications is lacking. With the development of fast-response N2O analyzers, the eddy covariance (EC) technique can be used to gather instantaneous measurements of N2O concentrations to quantify the exchange of nitrogen between the soil and atmosphere. The objectives of this study were to evaluate the performance of a new EC system, to measure the N2O flux with the system, and finally to examine relationships of the N2O flux with soil temperature, soil moisture, precipitation, and fertilization events. We assembled an EC system that included a sonic anemometer and a fast-response N2O analyzer (quantum cascade laser spectrometer) in a cornfield in Nolensville, Tennessee during the 2012 corn growing season (4 April–8 August). Fertilizer amounts totaling 217 kg N ha−1 were applied to the experimental site. The precision of the instrument was 0.066 ppbv for 10 Hz measurements. The seasonal mean detection limit of the N2O flux measurements was 2.10 ng N m−2 s−1. This EC system can be used to provide reliable N2O flux measurements. The cumulative emitted N2O for the entire growing season was 6.87 kg N2O-N ha−1. The 30 min average N2O emissions ranged from 0 to 11 100 μg N2O{-}N m−2 h−1 (mean = 257.5, standard deviation = 817.7). Average daytime emissions were much higher than night emissions (278.8 ± 865.8 vs. 100.0 ± 210.0 μg N2O-N m−2 h−1). Seasonal fluxes were highly dependent on soil moisture rather than soil temperature, although the diurnal flux was positively related to soil temperature. This study was one of the few experiments that continuously measured instantaneous, high-frequency N2O emissions in crop fields over a growing season of more than 100 days.