Mass Spectrometry-Based Proteomics Research to Fight COVID-19: An Expert Review on Hopes and Challenges

Author(s):  
Nasrin Amiri-Dashatan ◽  
Mehdi Koushki ◽  
Mostafa Rezaei-Tavirani
2020 ◽  
Vol 48 (5) ◽  
pp. 1953-1966
Author(s):  
Lindsay K. Pino ◽  
Jacob Rose ◽  
Amy O'Broin ◽  
Samah Shah ◽  
Birgit Schilling

Research into the basic biology of human health and disease, as well as translational human research and clinical applications, all benefit from the growing accessibility and versatility of mass spectrometry (MS)-based proteomics. Although once limited in throughput and sensitivity, proteomic studies have quickly grown in scope and scale over the last decade due to significant advances in instrumentation, computational approaches, and bio-sample preparation. Here, we review these latest developments in MS and highlight how these techniques are used to study the mechanisms, diagnosis, and treatment of human diseases. We first describe recent groundbreaking technological advancements for MS-based proteomics, including novel data acquisition techniques and protein quantification approaches. Next, we describe innovations that enable the unprecedented depth of coverage in protein signaling and spatiotemporal protein distributions, including studies of post-translational modifications, protein turnover, and single-cell proteomics. Finally, we explore new workflows to investigate protein complexes and structures, and we present new approaches for protein–protein interaction studies and intact protein or top-down MS. While these approaches are only recently incipient, we anticipate that their use in biomedical MS proteomics research will offer actionable discoveries for the improvement of human health.


2003 ◽  
Vol 8 (2) ◽  
pp. 60-61
Author(s):  
Toshifumi Takao

2006 ◽  
Vol 78 (18) ◽  
pp. 6614-6621 ◽  
Author(s):  
Huiling Liu ◽  
Yangjun Zhang ◽  
Jinglan Wang ◽  
Dong Wang ◽  
Chunxi Zhou ◽  
...  

Author(s):  
Aurélien Desmarchelier ◽  
Thomas Bessaire ◽  
Marie-Claude Savoy ◽  
Adrienne Tarres ◽  
Claudia Mujahid ◽  
...  

Abstract Background Veterinary drug residues in food are substances (>200 compounds) exhibiting potential health risks for consumers, thus being regulated in national legislations and the Codex Alimentarius. Most of the compounds are regulated based upon a maximum residue limit (MRL) while a few of them are banned in food for humans. The food sector needs a reliable and consensus analytical platform able to monitor these substances in a wide range of food commodities. Objective Several confirmatory methods based on liquid chromatography-mass spectrometry are available in the literature for either screening or quantification of veterinary drug residues in food, but usually applicable to limited scope of matrices. The current work describes the single-laboratory validation (SLV) of a method for screening 154 veterinary drug residues in several food categories. Methods This work describes a streamlined platform making use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening 105 antibiotics, 41 antiparasitics, 5 anti-inflammatory agents and 3 tranquilizers in foods of animal origin. For the best performance across the commodities (dairy-, meat-, fish- and egg-based materials) four method streams were established. As a screening tool, probabilities of detection (PODs) were assessed at the screening target concentration (STC < MRL) and the blank. Results The SLV led to PODs at the STC >94% and PODs in the blank < 4%. Conclusion Performance is in agreement with the acceptance criteria defined in SMPR 2018.010. Highlights The Expert Review Panel approved the present method as AOAC Official First Action 2020.04.


2000 ◽  
Vol 21 (11) ◽  
pp. 2252-2265 ◽  
Author(s):  
Thomas Keough ◽  
Martin P. Lacey ◽  
Angela M. Fieno ◽  
Raymond A. Grant ◽  
Yiping Sun ◽  
...  

2004 ◽  
Vol 22 (11) ◽  
pp. 1459-1466 ◽  
Author(s):  
Patrick G A Pedrioli ◽  
Jimmy K Eng ◽  
Robert Hubley ◽  
Mathijs Vogelzang ◽  
Eric W Deutsch ◽  
...  

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