Estimation of the level of endothelial factor of vascular growth and monocytic chemoattractant in children cerebral palsy

Objective. The aim of this article is to assess the role of vascular endothelial growth factor and monocytic chemoattractant in the development of cerebral palsy in children. Materials and methods. Examination of 77 children with different clinical forms of infantile cerebral palsy in the age group from 1 to 16 years was carried out. The content of monocyte chemoattractant (MCP) and vascular growth factor (VEGF) was determined in different forms of cerebral palsy. The obtained data were processed statistically. Results. The analysis of the obtained data revealed a significant increase in the indicators of monocytic chemoattractant and vascular growth factor in children with infantile cerebral palsy compared with healthy children. There were no significant differences between the indicators of MCP and VEGF in children with cerebral palsy and comorbid pathology and children with cerebral palsy without concomitant pathology. Conclusion. The authors of the presented analysis conclude that the determination of monocytic chemoattractant and vascular growth factor has a high diagnostic value for identifying a predisposition to the development of cerebral palsy. Timely detection of an increase in the level of these factors provides a broader prospective for early diagnosis of the disease and for the early implementation of rehabilitation measures accordingly.

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Low-potential immunosensor-based detection of the vascular growth factor 165 (VEGF165) using the nanocomposite platform of cobalt metal–organic framework

RSC Advances ◽

10.1039/d0ra03181j ◽

2020 ◽

Vol 10 (46) ◽

pp. 27288-27296

Author(s):

Sima Singh ◽

Arshid Numan ◽

Yiqiang Zhan ◽

Vijender Singh ◽

Aftab Alam ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Metal Organic Framework ◽

Cobalt Metal ◽

Vascular Endothelial ◽

Vascular Growth ◽

Vascular Growth Factor ◽

Metal Organic ◽

Endothelial Growth Factor ◽

Organic Framework

The vascular endothelial growth factor 165 (VEGF165) is a quintessential biomarker in cancers.

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Vascular Endothelial Growth Factor in Central Nervous System Injuries – A Vascular Growth Factor Getting Nervous?

Current Neurovascular Research ◽

10.2174/156720208786413451 ◽

2008 ◽

Vol 5 (4) ◽

pp. 246-259 ◽

Cited By ~ 11

Author(s):

Mattias Skold ◽

Martin Kanje

Keyword(s):

Central Nervous System ◽

Vascular Endothelial Growth Factor ◽

Nervous System ◽

Growth Factor ◽

Vascular Endothelial ◽

Vascular Growth ◽

Vascular Growth Factor ◽

Endothelial Growth Factor

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Inflammatory Choroidal Neovascularisation

10.47363/jorrr/2021(2)111 ◽

2021 ◽

pp. 1-3

Author(s):

Priyanka MS ◽

◽

Amber Kumar ◽

Keyword(s):

Growth Factor ◽

Multimodal Imaging ◽

Optical Coherence Tomography Angiography ◽

Visual Outcome ◽

Posterior Uveitis ◽

Vascular Endothelial ◽

Vascular Growth ◽

Vascular Growth Factor ◽

Endothelial Growth Factor ◽

The Ideal

Inflammatory choroidal neovascularization is uncommon severe sight threatening complication of uveitis, more frequent in posterior uveitis. Hypoxia, release of vascular endothelial growth factor and other mediators seem to be involved in its pathogenesis. Multimodal imaging including the recent optical coherence tomography angiography greatly aid in diagnosis and management. Management of these neovascular membranes consists of anti-vascular growth factor agents, with or without concomitant anti-inflammatory and/or corticosteroid therapy. Besides effective eradication of inflammation in uveitis, the ideal therapeutic goal should include timely detection and treatment of inflammatory CNVM, as the ultimate visual outcome would depend on the control of both

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XPS Modeling of Immobilized Recombinant Angiogenin and Apoliprotein A1 on Biodegradable Nanofibers

Nanomaterials ◽

10.3390/nano10050879 ◽

2020 ◽

Vol 10 (5) ◽

pp. 879

Author(s):

Anton Manakhov ◽

Elizaveta Permyakova ◽

Sergey Ershov ◽

Svetlana Miroshnichenko ◽

Mariya Pykhtina ◽

...

Keyword(s):

Tissue Engineering ◽

Growth Factor ◽

Photoelectron Spectroscopy ◽

Antioxidant Activities ◽

Fluorescent Microscopy ◽

X Ray ◽

Vascular Growth ◽

Vascular Growth Factor ◽

And Migration ◽

Protein Density

The immobilization of viable proteins is an important step in engineering efficient scaffolds for regenerative medicine. For example, angiogenin, a vascular growth factor, can be considered a neurotrophic factor, influencing the neurogenesis, viability, and migration of neurons. Angiogenin shows an exceptional combination of angiogenic, neurotrophic, neuroprotective, antibacterial, and antioxidant activities. Therefore, this protein is a promising molecule that can be immobilized on carriers used for tissue engineering, particularly for diseases that are complicated by neurotrophic and vascular disorders. Another highly important and viable protein is apoliprotein A1. Nevertheless, the immobilization of these proteins onto promising biodegradable nanofibers has not been tested before. In this work, we carefully studied the immobilization of human recombinant angiogenin and apoliprotein A1 onto plasma-coated nanofibers. We developed a new methodology for the quantification of the protein density of these proteins using X-ray photoelectron spectroscopy (XPS) and modeled the XPS data for angiogenin and apoliprotein A1 (Apo-A1). These findings were also confirmed by the analysis of immobilized Apo-A1 using fluorescent microscopy. The presented methodology was validated by the analysis of fibronectin on the surface of plasma-coated poly(ε-caprolactone) (PCL) nanofibers. This methodology can be expanded for other proteins and it should help to quantify the density of proteins on surfaces using routine XPS data treatment.

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Abstract 18332: EphrinB2/EphB4 Signaling controls the Dose-Dependent Switch between Normal and Aberrant Angiogenesis by VEGF

Circulation ◽

10.1161/circ.130.suppl_2.18332 ◽

2014 ◽

Vol 130 (suppl_2) ◽

Author(s):

Elena Groppa ◽

Veronica Sacchi ◽

Sime Brkic ◽

Marianna Trani ◽

Michael Heberer ◽

...

Keyword(s):

Growth Factor ◽

Longitudinal Splitting ◽

Vascular Growth ◽

Over Expression ◽

Capillary Networks ◽

Endothelial Proliferation ◽

Mouse Limb ◽

Normal Networks ◽

Endothelial Growth Factor

Vascular Endothelial Growth Factor-A (VEGF) is the master regulator of vascular growth and it can induce either normal or aberrant angiogenesis depending on its dose in the microenvironment around each producing cell in vivo, and not on the total amount. However, stimulation of pericyte recruitment by co-expression of Platelet Derived Growth Factor-BB (PDGF-BB) could prevent aberrant structures despite heterogeneous and high VEGF levels and switch to homogeneously normal angiogenesis. Here we dissected the role of specific pericyte-mediated signaling pathways in the switch between normal and aberrant angiogenesis by VEGF. Monoclonal populations of transduced myoblasts were used to homogeneously express specific VEGF doses, inducing either normal or aberrant angiogenesis, and were further transduced to secrete soluble blockers of the TGFβ-1/TGFβ-R, Tie2/Angiopoietin or EphB4/EphrinB2 pathways (LAP, sTie2Fc and sEphB4, respectively). Two weeks after implantation into mouse limb muscles, neither TGFβ nor Angiopoietin blockade altered the normal angiogenesis by low VEGF, whereas EphrinB2/EphB4 inhibition caused a switch to aberrant angioma-like structures, similar to the effects of blocking pericyte recruitment. Conversely, gain-of-function of EphB4 signaling by systemic treatment with recombinant EphrinB2-Fc completely prevented aberrant angiogenesis by high VEGF levels and yielded normal networks of mature capillaries. We recently found that VEGF over-expression in muscle induces angiogenesis without sprouting, but by circumferential enlargement and longitudinal splitting (intussusception). EphB4 inhibition increased both endothelial proliferation and the diameter of initial enlargements induced by low VEGF (4 days), leading to a failure of splitting and progressive angioma growth. However, it did not interfere with pericyte recruitment, contrary to high VEGF alone. Conversely, EphB4 stimulation decreased both endothelial proliferation and the diameter of enlargements induced by high VEGF to values similar to low VEGF alone, and accelerated splitting into pericyte-covered capillary networks. In conclusion, EphrinB2/EphB4 signaling can prevent VEGF-induced aberrant angiogenesis by regulating intussusception.

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Growth hormone/insulin-like growth factor-1 axis: a possible non-nutritional factor for growth retardation in children with cerebral palsy

Jornal de Pediatria ◽

10.2223/jped.2197 ◽

2012 ◽

Vol 88 (3) ◽

pp. 267-74 ◽

Cited By ~ 5

Author(s):

Moustafa A. Hegazi ◽

Othman E. Soliman ◽

Bothina M. Hasaneen ◽

Mohammed El-Arman ◽

Nawal Abd El-Galel ◽

...

Keyword(s):

Growth Hormone ◽

Cerebral Palsy ◽

Growth Factor ◽

Growth Retardation ◽

Insulin Like Growth Factor ◽

Children With Cerebral Palsy ◽

Nutritional Factor

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Oxygen Sensing and Vascular Growth Factor Gene Expression: Comparison with Erythropoietin Regulation

Clinical Science ◽

10.1042/cs088002pa ◽

1995 ◽

Vol 88 (s32) ◽

pp. 2P-2P

Author(s):

JM Gleadle ◽

BL Ebert ◽

JD Firth ◽

PJ Ratcliffe

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Oxygen Sensing ◽

Factor Gene ◽

Vascular Growth ◽

Growth Factor Gene ◽

Vascular Growth Factor

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Comparison of bispectral index values and depth of sedation during deep sedation using sevoflurane anesthesia in healthy children versus children with cerebral palsy

Nigerian Journal of Clinical Practice ◽

10.4103/njcp.njcp_553_18 ◽

2019 ◽

Vol 22 (6) ◽

pp. 801

Author(s):

G Kip ◽

P Onal ◽

N Oztas

Keyword(s):

Cerebral Palsy ◽

Bispectral Index ◽

Deep Sedation ◽

Healthy Children ◽

Sevoflurane Anesthesia ◽

Children With Cerebral Palsy

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VEGF and bFGF stimulate myocardial vascularization in embryonic chick

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1998.274.5.h1620 ◽

1998 ◽

Vol 274 (5) ◽

pp. H1620-H1626 ◽

Cited By ~ 15

Author(s):

Robert J. Tomanek ◽

Kapildeo Lotun ◽

Edward B. Clark ◽

Padma R. Suvarna ◽

Norman Hu

Keyword(s):

Growth Factor ◽

Volume Density ◽

Numerical Density ◽

Vascular Endothelial ◽

Vascular Volume ◽

Vascular Growth ◽

Spongy Layer ◽

Vessel Volume ◽

Electron And Light Microscopy ◽

Endothelial Growth Factor

We tested the hypothesis that early vascularization of the embryonic heart is enhanced after bolus injections of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) into the vitelline vein before the onset of myocardial vasculogenesis (3.5 days, stage 21). Electron and light microscopy were utilized to obtain morphometric data. At stages 29 and 31, myocardial vessel volume or numerical density were higher in embryos injected with 50 ng bFGF than in the saline-injected controls. A VEGF injection increased vascular volume density at stage 29 and both volume and numerical density at stage 31. bFGF, but not VEGF, was associated with an enhancement of the sinusoidal system (spongy layer of the ventricle) at stage 29. This effect disappeared by stage 31. In conclusion, 1) enhancement of bFGF or VEGF before myocardial vascularization increases vascular growth, but the initial effect of bFGF is greater; 2) the effects of these growth factors on vascular volume and numerical density are temporally dependent; and 3) bFGF, in addition to its effects on the coronary vasculature, influences ventricular modeling by apparently acting on myocytes as well as endothelial cells.

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