scholarly journals Histological Evaluation of Porous Additive-Manufacturing Titanium Artificial Bone in Rat Calvarial Bone Defects

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5360
Author(s):  
Naoko Imagawa ◽  
Kazuya Inoue ◽  
Keisuke Matsumoto ◽  
Michi Omori ◽  
Kayoko Yamamoto ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Bone Formation ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Artificial Bone ◽  
Calvarial Bone ◽  
Mixed Acid ◽  
Computed Tomography Images ◽  
Heat Treated

Jaw reconstruction using an additive-manufacturing titanium artificial bone (AMTAB) has recently attracted considerable attention. The synthesis of a titanium artificial bone is based on three-dimensional computed tomography images acquired before surgery. A histological evaluation of porous AMTAB (pAMTAB) embedded in rat calvarial bone defects was conducted. This study examined three groups: rats implanted with mixed-acid and heat-treated pAMTAB, rats implanted with untreated pAMTAB, and rats with no implant. In both pAMTAB groups, bone defects were created in rat calvarial bones using a 5-mm trephine bar, followed by pAMTAB implantation. The pAMTAB was fixed to the defect using the fitting force of the surrounding bones. The rats were sacrificed at 4, 8, and 16 weeks after implantation, and the skull was dissected. Undecalcified ground slides were prepared and stained with Villanueva Goldner. Compared with the no implant control group, both pAMTAB groups exhibited new bone formation inside the defect, with greater bone formation in the mixed-acid and heat-treated pAMTAB group than in the untreated pAMTAB group, but the difference was not significant. These data suggest that pAMTAB induces bone formation after implantation in bone defects. Bone formation appears to be enhanced by prior mixed-acid and heat-treated pAMTAB.

scholarly journals Osteogenic capacity of mixed-acid and heat-treated titanium mesh prepared by a selective laser melting technique

RSC Advances ◽  
2018 ◽  
Vol 8 (46) ◽  
pp. 26069-26077
Author(s):  
Kayoko Yamamoto ◽  
Seiji Yamaguchi ◽  
Tomiharu Matsushita ◽  
Shigeo Mori ◽  
Azumi Hirata ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Selective Laser Melting ◽  
Bone Defects ◽  
Titanium Mesh ◽  
Artificial Bone ◽  
Laser Melting ◽  
Mixed Acid ◽  
Heat Treated ◽  
Melting Technique

The practical use of additive manufacturing to create artificial bone as a material for repairing complex bone defects is currently attracting attention.

scholarly journals Bone healing in critically sized defects in rat calvaria, transplanted with chitosan alone, or associated with collagen and / or chondroitin sulfate:histological and histomorphometric pilot study

2021 ◽  
Vol 6 (1) ◽  
pp. 9-13
Author(s):  
Cynthia Chemaly ◽  
◽  
Mireille Kallassi ◽  
Rawad Samaran ◽  
Roy Abou Fadel ◽  
...  
Keyword(s):  
Bone Formation ◽  
Chondroitin Sulfate ◽  
Group Formation ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Sulfate Group ◽  
Control Group ◽  
Calvarial Bone ◽  
Group 2 ◽  
Group 1

Background: Chitosan is a natural biopolymer that has gained a special interest in bone regeneration in recent years. Objective: The objective of this study is to show the bone formation obtained following a transplantation of sponges of chitosan alone, chitosan combined with chondroitin sulfate or chitosan combined with chondroitin sulfate and collagen, in rat critical calvarial bone defects. Material and Methods: 12 Wistar rats were divided into 4 groups of 3 rats each. Critically sized bone defects were made in calvaria, and grafted by sponges of:collagen / chitosan / chondroitin (group 1), chitosan (group 2), chitosan / chondroitin sulfate (group3). Bone defects of group 4 remained empty for control. The animals were sacrificed 12 weeks after the surgery. Results: Histological analysis showed the formation of lamellar bone in the chitosan group. In the chitosan / chondroitin sulfate group, formation of a less mature bone than that of the chitosan group was also observed. However, the least bone formation was observed in the collagen / chitosan / chondroitin sulfate group. Histological sections showed the presence of fibrous tissue. The results for this group are similar to the control group. In groups 2 and 3, the materials appear completely resorbed while in group 1 the resorption of the matrix was incomplete. Conclusion: Despite the size of the sample, this study has shown that chitosan alone or in combination with chondroitin sulfate promotes bone formation. On the other hand, the combination chitosan / chondroitin sulfate / collagen showed a negative result.

scholarly journals Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Philipp S. Lienemann ◽  
Stéphanie Metzger ◽  
Anna-Sofia Kiveliö ◽  
Alain Blanc ◽  
Panagiota Papageorgiou ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Biological Processes ◽  
Micro Ct ◽  
Pinhole Spect

Abstract Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.

scholarly journals Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1993 ◽  
Author(s):  
Kunio Ishikawa ◽  
Youji Miyamoto ◽  
Akira Tsuchiya ◽  
Koichiro Hayashi ◽  
Kanji Tsuru ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Bone Substitutes ◽  
Physiological Solution ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Carbonate Apatite ◽  
Artificial Bone ◽  
New Bone Formation

Three commercially available artificial bone substitutes with different compositions, hydroxyapatite (HAp; Neobone®), carbonate apatite (CO3Ap; Cytrans®), and β-tricalcium phosphate (β-TCP; Cerasorb®), were compared with respect to their physical properties and tissue response to bone, using hybrid dogs. Both Neobone® (HAp) and Cerasorb® (β-TCP) were porous, whereas Cytrans® (CO3Ap) was dense. Crystallite size and specific surface area (SSA) of Neobone® (HAp), Cytrans® (CO3Ap), and Cerasorb® (β-TCP) were 75.4 ± 0.9 nm, 30.8 ± 0.8 nm, and 78.5 ± 7.5 nm, and 0.06 m2/g, 18.2 m2/g, and 1.0 m2/g, respectively. These values are consistent with the fact that both Neobone® (HAp) and Cerasorb® (β-TCP) are sintered ceramics, whereas Cytrans® (CO3Ap) is fabricated in aqueous solution. Dissolution in pH 5.3 solution mimicking Howship’s lacunae was fastest in CO3Ap (Cytrans®), whereas dissolution in pH 7.3 physiological solution was fastest in β-TCP (Cerasorb®). These results indicated that CO3Ap is stable under physiological conditions and is resorbed at Howship’s lacunae. Histological evaluation using hybrid dog mandible bone defect model revealed that new bone was formed from existing bone to the center of the bone defect when reconstructed with CO3Ap (Cytrans®) at week 4. The amount of bone increased at week 12, and resorption of the CO3Ap (Cytrans®) was confirmed. β-TCP (Cerasorb®) showed limited bone formation at week 4. However, a larger amount of bone was observed at week 12. Among these three bone substitutes, CO3Ap (Cytrans®) demonstrated the highest level of new bone formation. These results indicate the possibility that bone substitutes with compositions similar to that of bone may have properties similar to those of bone.

Bone Formation Effect of HA/β-TCP Composite Powders in Rabbit Calvarial Bone Defects: Histologic Study

2007 ◽  
pp. 1331-1334
Author(s):  
Hyun Seon Jang ◽  
Kwang Ho Lee ◽  
Moon Jin Jeong ◽  
Joo Cheol Park ◽  
Heung Joong Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defects ◽  
Histologic Study ◽  
Composite Powders ◽  
Calvarial Bone

scholarly journals Ginkgo Biloba improves bone formation during fracture healing: an experimental study in rats

2017 ◽  
Vol 25 (3) ◽  
pp. 95-98 ◽  
Author(s):  
Nizamettin Guzel ◽  
Emrah Sayit ◽  
Osman Aynaci ◽  
Servet Kerimoglu ◽  
Esin Yulug ◽  
...  
Keyword(s):  
Bone Formation ◽  
Fracture Healing ◽  
Ginkgo Biloba ◽  
Kirschner Wire ◽  
Ginkgo Biloba Extract ◽  
Histological Evaluation ◽  
Control Group ◽  
Sprague Dawley ◽  
Level Of Evidence ◽  
Significant Difference

ABSTRACT OBJECTIVES: Ginkgo biloba extract (EGb 761) is a plant extract obtained from the leaves of the G. biloba tree. The aim of this study was to assess the histological and radiological effects of G. biloba extract on fracture healing in an experimental fracture model using rat femurs. METHODS: Forty-eight female Sprague-Dawley rats (weight: 195-252 g; age: 20 weeks) were used in the study. The rats were randomly divided into six groups (n=8). A transverse fracture was made in the middle of the right femur of each rat and fixed with a Kirschner wire. The G. biloba groups received 60 mg/kg oral G. biloba extract once daily. No medication was given to the control groups. On days 7, 21 and 35, both sets of femurs were evaluated radiologically and histopathologically. RESULTS: Histological evaluation revealed that the G. biloba groups had significant differences at 21 and 35 days (p<0.05). The G. biloba group showed a significant difference in terms of bone formation on day 21 when compared to the control group (p<0.05). CONCLUSIONS: This study indicated that the use of G. biloba extract accelerated fracture healing. Both radiological and histological differences were detected, but the histological differences were more remarkable. Level of Evidence I, High Quality Randomized Trial.

Bone formation effect of HA/β-TCP composite powders in rabbit calvarial bone defects: Histologic study

2006 ◽  
Vol 36 (1) ◽  
pp. 1 ◽  
Author(s):  
Kwang-Ho Lee ◽  
Hyun-Seon Jang ◽  
Joo-Cheol Park ◽  
Heung-Joong Kim ◽  
Chong-Kwan Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defects ◽  
Histologic Study ◽  
Composite Powders ◽  
Calvarial Bone

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects

2019 ◽  
Vol 207 (3-4) ◽  
pp. 138-148 ◽  
Author(s):  
Rubia Teodoro Stuepp ◽  
Priscilla Barros Delben ◽  
Filipe Modolo ◽  
Andrea Gonçalves Trentin ◽  
Ricardo Castilho Garcez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Treated Group ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Mandibular Bone ◽  
Significant Difference ◽  
Human Dental Pulp

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.

scholarly journals The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

2017 ◽  
Vol 203 (6) ◽  
pp. 327-338 ◽  
Author(s):  
Min Wook Joo ◽  
Seung Jae Chung ◽  
Seung Han Shin ◽  
Yang-Guk Chung
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Micro Ct ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.

Sign in / Sign up

Export Citation Format

Share Document