Analysis of the Biosynthesis of Glycopeptide Antibiotics: Basis for Creating New Structures by Combinatorial Biosynthesis

2005 ◽  
pp. 139-150
Author(s):  
Stefan Pelzer ◽  
Wolfgang Wohlleben
Keyword(s):  
Combinatorial Biosynthesis ◽  
Glycopeptide Antibiotics

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Expanding the Scope of Aromatic Polyketides by Combinatorial Biosynthesis

2003 ◽  
Vol 6 (6) ◽  
pp. 501-512 ◽  
Author(s):  
J. Kantola ◽  
T. Kunnari ◽  
P. Mantsala ◽  
K. Ylihonko
Keyword(s):  
Combinatorial Biosynthesis ◽  
Aromatic Polyketides

Affinity chromatography of glycopeptide antibiotics

1987 ◽  
Vol 392 ◽  
pp. 225-238 ◽  
Author(s):  
Gail Folena-Wasserman ◽  
Robert D. Sitrin ◽  
Frederic Chapin ◽  
Kenneth M. Snader
Keyword(s):  
Affinity Chromatography ◽  
Glycopeptide Antibiotics

scholarly journals Inhibition of feline (FIPV) and human (SARS) coronavirus by semisynthetic derivatives of glycopeptide antibiotics

2006 ◽  
Vol 72 (1) ◽  
pp. 20-33 ◽  
Author(s):  
Jan Balzarini ◽  
Els Keyaerts ◽  
Leen Vijgen ◽  
Herman Egberink ◽  
Erik De Clercq ◽  
...  
Keyword(s):  
Glycopeptide Antibiotics ◽  
Sars Coronavirus ◽  
Derivatives Of

Glycopeptide Antibiotics (Dalbahep tides)

2001 ◽  
pp. 369-396
Author(s):  
Giancarlo Lancini ◽  
Bruno Cavalleri
Keyword(s):  
Glycopeptide Antibiotics

Fluorescence assay to predict activity of the glycopeptide antibiotics

2018 ◽  
Vol 72 (2) ◽  
pp. 114-117 ◽  
Author(s):  
Vladimir Vimberg ◽  
Radek Gazak ◽  
Zsolt Szűcs ◽  
Aniko Borbás ◽  
Pal Herczegh ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Glycopeptide Antibiotics

scholarly journals Halogenation of glycopeptide antibiotics occurs at the amino acid level during non-ribosomal peptide synthesis

2017 ◽  
Vol 8 (9) ◽  
pp. 5992-6004 ◽  
Author(s):  
Tiia Kittilä ◽  
Claudia Kittel ◽  
Julien Tailhades ◽  
Diane Butz ◽  
Melanie Schoppet ◽  
...  
Keyword(s):  
Amino Acid ◽  
Peptide Synthesis ◽  
Acid Level ◽  
Amino Acid Level ◽  
Antibiotic Biosynthesis ◽  
Carrier Protein ◽  
Glycopeptide Antibiotics ◽  
Glycopeptide Antibiotic ◽  
Protein Substrates

Halogenase enzymes involved in glycopeptide antibiotic biosynthesis accept aminoacyl-carrier protein substrates.

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