Gastrointestinal delivery of glipizide from carboxymethyl locust bean gum-Al3+-alginate hydrogel network: In vitro and in vivo performance

2012 ◽  
pp. n/a-n/a ◽  
Author(s):  
Paramita Dey ◽  
Sabyasachi Maiti ◽  
Biswanath Sa
Keyword(s):  
Locust Bean Gum ◽  
Alginate Hydrogel ◽  
Hydrogel Network ◽  
Locust Bean

scholarly journals Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

2017 ◽  
Vol 96 ◽  
pp. 786-797 ◽  
Author(s):  
Luis Braz ◽  
Ana Grenha ◽  
Domingos Ferreira ◽  
Ana M. Rosa da Costa ◽  
Carlos Gamazo ◽  
...  
Keyword(s):  
Oral Immunization ◽  
Locust Bean Gum ◽  
In Vivo Evaluation ◽  
Locust Bean

Abstract 558: Microchanneled Polysaccharide Hydrogel Laden With Endothelial Cells and Mesenchymal Stem Cells With VEGF Facilitate Formation of Vascular Structures and Are Effective for Repairing Tissue Ischemia

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Sangho Lee ◽  
Min Kyung Lee ◽  
Hyunjoon Kong ◽  
Young-sup Yoon
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Survival ◽  
Vascular Structure ◽  
Hindlimb Ischemia ◽  
Alginate Hydrogel ◽  
Vessel Formation

Various hydrogels are used to create vascular structure in vitro or to improve cell engraftment to overcome low cell survival in vivo, a main hurdle for bare cell therapy Recently we developed a modified alginate hydrogel within which microchannels are aligned to guide the direction and spatial organization of loaded cells. We investigated whether these cell constructs in which HUVECs and human mesenchymal stem cells (hMSCs) are co-loaded in this novel microchanneled hydrogel facilitate formation of vessels in vitro and in vivo, and enhance recovery of hindlimb ischemia. We crafted a modified alginate hydrogel which has microchannels, incorporates a cell adhesion peptide RGD, and was encapsulated with VEGF. We then compared vascular structure formation between the HUVEC only (2 x 105 cells) group and the HUVEC plus hMSC group. In the HUVEC+hMSC group, we mixed HUVECs and hMSCs at the ratio of 3:1. For cell tracking, we labeled HUVECs with DiO, a green fluorescence dye. After loading cells into the microchannels of the hydrogel, these constructs were cultured for seven days and were examined by confocal microscopy. In the HUVEC only group, HUVECs stands as round shaped cells without forming tubular structures within the hydrogel. However, in the HUVEC+hMSC group, HUVECs were stretched out and connected with each other, and formed vessel-like structure following pre-designed microchannels. These results suggested that hMSCs play a critical role for vessel formation by HUVECs. We next determined their in vivo effects using a mouse hindlimb ischemia model. We found that engineered HUVEC+hMSC group showed significantly higher perfusion over 4 weeks compared to the engineered HUVEC only group or bare cell (HUVEC) group. Confocal microscopic analysis of harvested tissues showed more robust vessel formation within and outside of the cell constructs and longer term cell survival in HUVEC+hMSC group compared to the other groups. In conclusion, this novel microchanneled alginate hydrogel facilitates aligned vessel formation of endothelial cells when combined with MSCs. This vessel-embedded hydrogel constructs consisting of HUVECs and MSCs contribute to perfusable vessel formation, prolong cell survival in vivo, and are effective for recovering limb ischemia.

Binding of Mineral Elements to Locust Bean Gum Influences Availability In Vitro

2001 ◽  
Vol 81 (1) ◽  
pp. 79-92 ◽  
Author(s):  
Douwina Bosscher ◽  
Harry Robberecht ◽  
Rudy Van Cauwnebergh ◽  
Micheline Van Caillie-Bertrand ◽  
Hendrik Deelstra
Keyword(s):  
Mineral Elements ◽  
Locust Bean Gum ◽  
Locust Bean

scholarly journals CRISPR-Cas9 modified bacteriophage for treatment of Staphylococcus aureus induced osteomyelitis and soft tissue infection

2019 ◽  
Author(s):  
Leah K. Horstemeyer ◽  
JooYoun Park ◽  
Elizabeth A. Swanson ◽  
Mary Catherine Beard ◽  
Emily M. McCabe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Bone Infection ◽  
Tissue Infection ◽  
Fluorescent Imaging ◽  
Alginate Hydrogel ◽  
Antibiotic Resistant

AbstractOsteomyelitis, or bone infection, is often induced by antibiotic resistant Staphylococcus aureus strains of bacteria. Although debridement and long-term administration of antibiotics are the gold standard for osteomyelitis treatment, the increase in prevalence of antibiotic resistant bacterial strains limits the ability of clinicians to effectively treat infection. Bacteriophages (phages), viruses that effectively lyse bacteria, have gained recent attention for their high specificity, non-toxicity, and the low likelihood of resistance development by pathogens. Previously, we have shown that CRISPR-Cas9 genomic editing techniques could be utilized to expand bacteriophage host range and enhance bactericidal activity through modification of the tail fiber protein, as well as improve safety with removal of major virulence genes. In a dermal infection study, these CRISPR-Cas9 phages reduced bacterial load relative to unmodified phage. Thus, we hypothesized this bacteriophage would be effective to mitigate infection from a biofilm forming S. aureus strain in vitro and in vivo. In vitro, qualitative fluorescent imaging demonstrated superiority of phage to conventional vancomycin and fosfomycin antibiotics against S. aureus biofilm. Quantitative antibiofilm effects increased over time for fosfomycin, phage, and fosfomycin-phage (dual) therapeutics delivered via alginate hydrogel. We developed an in vivo rat model of osteomyelitis and soft tissue infection that was reproducible and challenging and enabled longitudinal monitoring of infection progression. Using this model, phage (with and without fosfomycin) delivered via alginate hydrogel were successful in reducing soft tissue infection but not bone infection, based on bacteriological, histological, and scanning electron microscopy analyses. Notably, the efficacy of phage at mitigating soft tissue infection was equal to that of high dose fosfomycin. Future research may utilize this model as a platform for evaluation of therapeutic type and dose, and alternate delivery vehicles for osteomyelitis mitigation.

Evaluation of co-cultured spermatogonial stem cells encapsulated in alginate hydrogel with Sertoli cells and their transplantation into azoospermic mice

Zygote ◽  
2021 ◽  
pp. 1-8
Author(s):  
Mohammad Veisi ◽  
Kamran Mansouri ◽  
Vahideh Assadollahi ◽  
Cyrus Jalili ◽  
Afshin Pirnia ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Periodic Acid ◽  
Three Dimensional ◽  
3D Culture ◽  
Alginate Hydrogel ◽  
Periodic Acid Schiff ◽  
Expression Levels ◽  
Pas Staining

Summary An in vitro spermatogonial stem cell (SSC) culture can serve as an effective technique to study spermatogenesis and treatment for male infertility. In this research, we compared the effect of a three-dimensional alginate hydrogel with Sertoli cells in a 3D culture and co-cultured Sertoli cells. After harvest of SSCs from neonatal mice testes, the SSCs were divided into two groups: SSCs on a 3D alginate hydrogel with Sertoli cells and a co-culture of SSCs with Sertoli cells for 1 month. The samples were evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays and bromodeoxyuridine (BrdU) tracing, haematoxylin and eosin (H&E) and periodic acid–Schiff (PAS) staining after transplantation into an azoospermic testis mouse. The 3D group showed rapid cell proliferation and numerous colonies compared with the co-culture group. Molecular assessment showed significantly increased integrin alpha-6, integrin beta-1, Nanog, Plzf, Thy-1, Oct4 and Bcl2 expression levels in the 3D group and decreased expression levels of P53, Fas, and Bax. BrdU tracing, and H&E and PAS staining results indicated that the hydrogel alginate improved spermatogenesis after transplantation in vivo. This finding suggested that cultivation of SSCs on alginate hydrogel with Sertoli cells in a 3D culture can lead to efficient proliferation and maintenance of SSC stemness and enhance the efficiency of SSC transplantation.

Alginate hydrogel containing hydrogen sulfide as the functional wound dressing material: In vitro and in vivo study

2020 ◽  
Vol 164 ◽  
pp. 3323-3331 ◽  
Author(s):  
Simin Nazarnezhada ◽  
Ghasem Abbaszadeh-Goudarzi ◽  
Hadi Samadian ◽  
Mehdi Khaksari ◽  
Jila Majidi Ghatar ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Wound Dressing ◽  
In Vivo Study ◽  
Alginate Hydrogel
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