Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering

2016 ◽  
Vol 11 (7) ◽  
pp. 932-944 ◽  
Author(s):  
Mojtaba Parvizi ◽  
Lydia A.M. Bolhuis-Versteeg ◽  
André A. Poot ◽  
Martin C. Harmsen
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Growth Factors ◽  
Smooth Muscle Cells ◽  
Mechanical Stimulation ◽  
Stromal Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Vascular Tissue Engineering ◽  
Adipose Derived Stromal Cells

Effective seeding of smooth muscle cells into tubular poly(trimethylene carbonate) scaffolds for vascular tissue engineering

2010 ◽  
Vol 95A (2) ◽  
pp. 440-446 ◽  
Author(s):  
Y. Song ◽  
J. W. H. Wennink ◽  
M. M. J. Kamphuis ◽  
I. Vermes ◽  
A. A. Poot ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Vascular Tissue Engineering ◽  
Trimethylene Carbonate

The Effect of Dynamical Strain on the Maturation of Collagen-Based Cell-Containing Scaffolds for Vascular Tissue Engineering

2011 ◽  
Vol 409 ◽  
pp. 152-157 ◽  
Author(s):  
Lucie Levesque ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Collagen Fibrils ◽  
Vascular Tissue Engineering ◽  
Silicone Membrane ◽  
Custom Made

Diseases occurring to blood vessel are preferentially solved by replacing the vessel by an autologous graft. When it is not available, a synthetic graft is used which has low patency rates for small diameter (<6 mm) vessels. Tissue engineering of blood vessel aims to improve the performance of vascular substitutes. Bioreactors are used in vascular tissue engineering to mimic the mechanical and biochemical environment of blood vessel. A 2D bioreactor was custom made in order to impose a dynamical strain to silicone membrane receiving the collagen cell-based construct. Collagen gels with vascular smooth muscle cells cultured inside were subdued to maturation under dynamical uniaxial stretch regimes at 1Hz for 48 hours. The percentage of deformation encountered by the silicone membrane was measured by ImageJ. Collagen fibrils and porcine smooth muscle cells (PSMC) orientations were assessed by scanning electron microscopy (SEM). Results show that the study of mechanical conditioning on cell activity is an important issue for enhancing the alignment of collagen fibrils.

scholarly journals Promoting Tropoelastin Expression in Arterial and Venous Vascular Smooth Muscle Cells and Fibroblasts for Vascular Tissue Engineering

2016 ◽  
Vol 22 (10) ◽  
pp. 923-931 ◽  
Author(s):  
Tonia C. Rothuizen ◽  
Raymond Kemp ◽  
Jacques M.G.J. Duijs ◽  
Hetty C. de Boer ◽  
Roel Bijkerk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Vascular Tissue Engineering

scholarly journals Dynamic Culturing of Smooth Muscle Cells in Tubular Poly(Trimethylene Carbonate) Scaffolds for Vascular Tissue Engineering

2011 ◽  
Vol 17 (3-4) ◽  
pp. 381-387 ◽  
Author(s):  
Yan Song ◽  
Jos W.H. Wennink ◽  
Marloes M.J. Kamphuis ◽  
Lotus M.T. Sterk ◽  
Istvan Vermes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Vascular Tissue Engineering ◽  
Trimethylene Carbonate

Atomic Force and Confocal Microscopic Studies of Collagen-Cell-Based Scaffolds for Vascular Tissue Engineering

2006 ◽  
Vol 15-17 ◽  
pp. 83-88 ◽  
Author(s):  
Luca Amadori ◽  
Navneeta Rajan ◽  
Simone Vesentini ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Collagen Gel ◽  
Muscle Cells ◽  
Vascular Tissue Engineering ◽  
Vascular Cells ◽  
Atomic Force

Collagen is the most used naturally occurring scaffold material. It’s a structural protein ubiquitous among mammalian. The ability of collagen type I to host different cell phenotype in vitro and its low antigenecity in vivo are well known. However, the principal drawback of collagenbased materials consists in their low mechanical properties. For vascular tissue engineering this represents a major limit, as the aim is to mimic the structure of a native vessel, which is known to be resistant and viscoelastic. Moreover, vascular cells are known to be susceptible in vivo to reorganize the matrix in which they proliferate. Therefore, the aim of this project is to study the micro structural organization of collagen-based scaffolds, and to assess the interactions between collagen and smooth muscle cells during regeneration. This knowledge will then allow the development of appropriate strategies to tailor the microstructure of the scaffold and its properties. Smooth muscle cells (SMCs) were selected to study the interactions between cells and matrix during the proliferation. Atomic Force Microscopy (AFM) in dry state in tapping mode and Confocal Laser Scanning Microscopy (CLSM) in reflection mode were used to investigate the microstructure of the scaffold. For the former technique cells were seeded on top of the collagen gel after jellification, while for the latter, cells were embedded into the collagen gel and stained with Rhodamine. The contact points between matrix and cells were investigated, as well as the capacity of vascular cells to induce a structural reorganization of collagen fibrils in the scaffold.

Genetic Modification of Smooth Muscle Cells to Control Phenotype and Function in Vascular Tissue Engineering

2004 ◽  
Vol 10 (1-2) ◽  
pp. 189-199 ◽  
Author(s):  
Jan P. Stegemann ◽  
Nupur B. Dey ◽  
Thomas M. Lincoln ◽  
Robert M. Nerem
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Genetic Modification ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Vascular Tissue Engineering ◽  
And Function

scholarly journals Local delivery of a collagen-binding FGF-1 chimera to smooth muscle cells in collagen scaffolds for vascular tissue engineering

Biomaterials ◽  
2010 ◽  
Vol 31 (5) ◽  
pp. 878-885 ◽  
Author(s):  
Yonggang Pang ◽  
Xiaoli Wang ◽  
Areck A. Ucuzian ◽  
Eric M. Brey ◽  
Wilson H. Burgess ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Local Delivery ◽  
Vascular Tissue Engineering ◽  
Collagen Scaffolds ◽  
Collagen Binding
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