Characterization of three‐dimensional rat central nervous system culture maturation, with applications to monitor cholinergic integrity

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Parker L. Andersen ◽  
Patrick Vermette ◽  
Abdelouahed Khalil ◽  
Jacek M. Witkowski ◽  
Tamas Fülöp
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

scholarly journals Molecular Modeling of Histamine Receptors—Recent Advances in Drug Discovery

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1778
Author(s):  
Pakhuri Mehta ◽  
Przemysław Miszta ◽  
Sławomir Filipek
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Molecular Modeling ◽  
Molecular Targets ◽  
Histamine Receptors ◽  
Experimental Characterization ◽  
Complex Disorders ◽  
Theoretical Investigations ◽  
Recent Developments

The recent developments of fast reliable docking, virtual screening and other algorithms gave rise to discovery of many novel ligands of histamine receptors that could be used for treatment of allergic inflammatory disorders, central nervous system pathologies, pain, cancer and obesity. Furthermore, the pharmacological profiles of ligands clearly indicate that these receptors may be considered as targets not only for selective but also for multi-target drugs that could be used for treatment of complex disorders such as Alzheimer’s disease. Therefore, analysis of protein-ligand recognition in the binding site of histamine receptors and also other molecular targets has become a valuable tool in drug design toolkit. This review covers the period 2014–2020 in the field of theoretical investigations of histamine receptors mostly based on molecular modeling as well as the experimental characterization of novel ligands of these receptors.

scholarly journals Immunological and Molecular Characterization of Goα-like Proteins in the Drosophila Central Nervous System

1989 ◽  
Vol 264 (31) ◽  
pp. 18552-18560 ◽  
Author(s):  
N C Thambi ◽  
F Quan ◽  
W J Wolfgang ◽  
A Spiegel ◽  
M Forte
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Molecular Characterization

Three-dimensional sonographic features of fetal central nervous system anomaly

2000 ◽  
Vol 79 (8) ◽  
pp. 635-639 ◽  
Author(s):  
TOSHIYUKI HATA ◽  
TOSHIHIRO YANAGIHARA ◽  
MINAKO MATSUMOTO ◽  
UIKO HANAOKA ◽  
MARI UETA ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Central Nervous System Anomaly

scholarly journals Molecular cloning and analysis of l(1)ogre, a locus of Drosophila melanogaster with prominent effects on the postembryonic development of the central nervous system.

Genetics ◽  
1990 ◽  
Vol 126 (4) ◽  
pp. 1033-1044 ◽  
Author(s):  
T Watanabe ◽  
D R Kankel
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Postembryonic Development ◽  
P Element ◽  
Reading Frame ◽  
Isolation And Characterization ◽  
The Central Nervous System ◽  
Highly Hydrophobic ◽  
Conceptual Translation

Abstract Previous genetic studies have shown that wild-type function of the l(1)ogre (lethal (1) optic ganglion reduced) locus is essential for the generation and/or maintenance of the postembryonic neuroblasts including those from which the optic lobe is descended. In the present study molecular isolation and characterization of the l(1)ogre locus was carried out to study the structure and expression of this gene in order to gain information about the nature of l(1)ogre function and its relevance to the development of the central nervous system. About 70 kilobases (kb) of genomic DNA were isolated that spanned the region where l(1)ogre was known to reside. Southern analysis of a l(1)ogre mutation and subsequent P element-mediated DNA transformation mapped the l(1)ogre+ function within a genomic fragment of 12.5 kb. Northern analyses showed that a 2.9-kb message transcribed from this 12.5-kb region represented l(1)ogre. A 2.15-kb portion of a corresponding cDNA clone was sequenced. An open reading frame (ORF) of 1,086 base paris was found, and a protein sequence of 362 amino acids with one highly hydrophobic segment was deduced from conceptual translation of this ORF.

scholarly journals Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System

2016 ◽  
Vol 9 ◽  
Author(s):  
Marleen H. van Coevorden-Hameete ◽  
Maarten J. Titulaer ◽  
Marco W. J. Schreurs ◽  
Esther de Graaff ◽  
Peter A. E. Sillevis Smitt ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Surface ◽  
Neuronal Cell ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
The Central Nervous System

Characterization of placental luteinizing hormone-relasing factor-like material

1981 ◽  
Vol 96 (3) ◽  
pp. 394-397 ◽  
Author(s):  
Jau-Nan Lee ◽  
Markku Seppälä ◽  
Tim Chard
Keyword(s):  
Central Nervous System ◽  
High Pressure ◽  
Nervous System ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Luteinizing Hormone ◽  
Human Placenta ◽  
The Central Nervous System ◽  
Inhibition Curve

Abstract. High pressure liquid chromatography (HPLC) and radioimmunoassay were employed to characterize luteinizing hormone-releasing factor (LRF)-like material in the human placenta. Methanol extracts of the placenta were washed with acetic acid and chloroform, further purified on coarse octadecylsilane columns, fractionated on HPLC, and tested by radioimmunoassay. In HPLC, placental LRF had the same retention time as synthetic LRF, and such fractions gave an inhibition curve which was parallel to that of synthetic LRF in radioimmunoassav. It is concluded that human placental I.RF is similar or identical to LRF in the central nervous system.

Isolation and characterization of periaxolemmal and axolemmal enriched membrane fractions from the rat central nervous system

1992 ◽  
Vol 32 (4) ◽  
pp. 593-604 ◽  
Author(s):  
V. S. Sapirstein ◽  
R. Durrie ◽  
B. Cherksey ◽  
M. E. Beard ◽  
C. J. Flynn ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Isolation And Characterization
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