Radio-modifying potential ofSaraca indicaagainst ionizing radiation: an in vitro study using Chinese hamster lung fibroblast (V79) cells

2015 ◽  
Vol 39 (9) ◽  
pp. 1061-1072 ◽  
Author(s):  
Shubhankar Das ◽  
Rishikesh Kumar ◽  
Bola Sadashiva Satish Rao
Keyword(s):  
Ionizing Radiation ◽  
Chinese Hamster ◽  
In Vitro Study ◽  
Lung Fibroblast ◽  
Vitro Study ◽  
V79 Cells ◽  
Chinese Hamster Lung Fibroblast

Growth inhibition of cartilaginous embryonic chick tibiae by ionizing radiation: an in vitro study

1988 ◽  
Vol 1 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Leo De Ridder ◽  
Hubert Thierens ◽  
Maria Cornelissen
Keyword(s):  
Ionizing Radiation ◽  
Growth Inhibition ◽  
In Vitro Study ◽  
Vitro Study ◽  
Embryonic Chick

Indium chloride-induced micronuclei via reactive oxygen species in Chinese hamster lung fibroblast V79 cells

2011 ◽  
Vol 28 (10) ◽  
pp. 595-600 ◽  
Author(s):  
Ruey-Hseng Lin ◽  
Ming-Ling Yang ◽  
Yi-Ching Li ◽  
Hui-Min Chang ◽  
Yu-Hsiang Kuan
Keyword(s):  
Reactive Oxygen Species ◽  
Chinese Hamster ◽  
Reactive Oxygen ◽  
Lung Fibroblast ◽  
Oxygen Species ◽  
V79 Cells ◽  
Indium Chloride ◽  
Chinese Hamster Lung Fibroblast

scholarly journals In Vitro Study of Encapsulation Therapy for Fabry Disease Using Genetically Engineered CHO Cell Line

2002 ◽  
Vol 11 (4) ◽  
pp. 325-329 ◽  
Author(s):  
Y. Naganawa ◽  
K. Ohsugi ◽  
R. Kase ◽  
I. Date ◽  
H. Sakuraba ◽  
...  
Keyword(s):  
Fabry Disease ◽  
Cell Line ◽  
Cho Cells ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
In Vitro Study ◽  
Vitro Study ◽  
Polymeric Membrane ◽  
Novel Approach

Fabry disease is an X-linked recessive disorder caused by a deficiency of the lysosomal hydrolase α-galac-tosidase A (α-gal). The deficiency of this enzyme leads to the systemic deposition of ceramide trihexoside (CTH) in various tissues and organs. Enzyme replacement using IV doses of recombinant human α-gal produced in CHO cells or in human fibroblasts is currently being evaluated in clinical trials as a potential therapy for this disease. However, it requires lifelong therapy involving a large amount of purified α-gal. As a novel approach for treatment of Fabry disease we used polymer encapsulated Chinese hamster ovary (CHO) cells genetically modified to express α-gal. The secreted high levels of α-gal passed through the semipermeable polymeric membrane. Using coculture system with Fabry fibroblasts, the secreted enzyme was taken up in cells, resulting in reduced accumulation of CTH in Fabry fibroblasts. This in vitro study demonstrated that an encapsulated α-gal-secreting cell line can be used to treat Fabry mice by transplantation in vivo. Judging from the protection against immune rejection by a semipermeable synthetic membrane, this novel approach may be applied to treat patients with Fabry disease and other lysosomal storage diseases.

Sign in / Sign up

Export Citation Format

Share Document