scholarly journals Rational design of thiolated polyenes as trifunctional Raman reporter molecules in surface‐enhanced Raman scattering nanotags for cytokine detection in a lateral flow assay

2020 ◽  
Vol 13 (6) ◽  
Author(s):  
Thomas Keller ◽  
Svetlana Brem ◽  
Vi Tran ◽  
Oliver Sritharan ◽  
Daniel Schäfer ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Rational Design ◽  
Surface Enhanced Raman Scattering ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Cytokine Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

A surface-enhanced Raman scattering-based lateral flow immunosensor for colistin in raw milk

2019 ◽  
Vol 282 ◽  
pp. 703-711 ◽  
Author(s):  
Yu Li ◽  
Shusheng Tang ◽  
Wanjun Zhang ◽  
Xiaojin Cui ◽  
Yuxin Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Raw Milk ◽  
Surface Enhanced Raman Scattering ◽  
Lateral Flow ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Duplex Surface Enhanced Raman Scattering-Based Lateral Flow Immunosensor for the Low-Level Detection of Antibiotic Residues in Milk

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5249
Author(s):  
Ruiqi Fan ◽  
Shusheng Tang ◽  
Sunlin Luo ◽  
Hu Liu ◽  
Wanjun Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Simultaneous Detection ◽  
Surface Enhanced Raman Scattering ◽  
Lateral Flow ◽  
Antibiotic Residues ◽  
Relative Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Analytical Approaches

A duplex surface enhanced Raman scattering (SERS)-based lateral flow immunosensor was established for the simultaneous detection of two common antibiotic residues including tetracycline and penicillin in milk. The newly synthesized Au@Ag nanoparticles were labeled with different Raman molecules including 5,5-dithiobis-2-nitrobenzoic acid (DTNB) or 4-mercaptobenzoic acid (MBA), followed by the conjugation of anti-tetracycline monoclonal antibody or anti-penicillin receptor, forming two kinds of SERS nanoprobes. The two nanoprobes can recognize tetracycline-BSA and ampicillin-BSA, respectively, which facilitates the simultaneous detection of the two types of antibiotics on a single test line. After optimization, detection limits of tetracycline and penicillin as low as 0.015 ng/mL and 0.010 ng/mL, respectively, were achieved. These values were far below those of most of other documented bio-analytical approaches. Moreover, the spiking test demonstrates an excellent assay accuracy with recoveries of 88.8% to 111.3%, and satisfactory assay precision with relative standard deviation below 16%. Consequently, the results demonstrate that the SERS-based lateral flow immunosensor developed in this study has the advantages of excellent assay sensitivity and remarkable multiplexing capability, thus it will have great application potential in food safety monitoring.

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