Alterations in the rate of hemoglobin synthesis during chick embryogenesis

1972 ◽  
Vol 79 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Frederick Grinnell ◽  
John C. Lee
Keyword(s):  
Hemoglobin Synthesis ◽  
Chick Embryogenesis

scholarly journals PRIMITIVE ERYTHROPOIESIS IN EARLY CHICK EMBRYOGENESIS

1971 ◽  
Vol 50 (3) ◽  
pp. 652-668 ◽  
Author(s):  
Harold Weintraub ◽  
Graham Le M. Campbell ◽  
Howard Holtzer
Keyword(s):  
Cell Cycle ◽  
Blood Cells ◽  
Generation Time ◽  
Cell Lineage ◽  
Embryonic Chick ◽  
Average Cell ◽  
Hemoglobin Synthesis ◽  
Chick Embryogenesis ◽  
Primitive Erythropoiesis ◽  
Mitotic Behavior

The primitive line of embryonic chick blood cells develop as a relatively homogeneous cohort of cells. Using an analysis based on the continuous uptake of thymidine-3H, we have established the generation time, G1, S, and G2 for progressively more mature generations of these immature erythroblasts. The data indicate that after the initiation of hemoglobin synthesis, the average cell will yield six generations of hemoglobin producing erythroblasts. The older generations of erythroblasts exhibit a longer generation time, G1, S, and G2 than the earlier generations of erythroblasts. Other methods of analysis corroborated these findings. One of these methods, an estimate of total erythrocyte productivity from the primitive stem cells (hematocytoblasts), led to the conclusion that the erythroblast cell lineage might be initiated as early as the sixth or seventh division following fertilization. In addition, primitive erythroblasts characterized by one set of cell cycle parameters, when grown in serum associated with erythroblasts of different parameters, showed no alteration in mitotic behavior. These results suggest the presence of programmed cell division not immediately cued by extracellular influence.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

scholarly journals THE RELATION OF PYRROLE-CONTAINING PIGMENTS TO HEMOGLOBIN SYNTHESIS

1939 ◽  
Vol 128 (2) ◽  
pp. 501-509
Author(s):  
G.O. Kohler ◽  
C.A. Elvehjem ◽  
E.B. Hart
Keyword(s):  
Hemoglobin Synthesis

scholarly journals Control of Hemoglobin Synthesis in the Cultured Chick Blastoderm

1967 ◽  
Vol 242 (8) ◽  
pp. 1903-1911
Author(s):  
Richard D. Levere ◽  
S. Granick
Keyword(s):  
Chick Blastoderm ◽  
Hemoglobin Synthesis

scholarly journals Regulation of Hemoglobin Synthesis

1974 ◽  
Vol 249 (20) ◽  
pp. 6517-6526
Author(s):  
Wallace L. McKeehan
Keyword(s):  
Hemoglobin Synthesis

scholarly journals Regulation of Hemoglobin Synthesis

1972 ◽  
Vol 247 (23) ◽  
pp. 7822-7829 ◽  
Author(s):  
Harvey F. Lodish ◽  
David G. Nathan
Keyword(s):  
Hemoglobin Synthesis

Hemoglobin synthesis in isolated erythroid colonies from the chick embryo

1976 ◽  
Vol 49 (2) ◽  
pp. 556-562 ◽  
Author(s):  
Kay S. Pine ◽  
Allan J. Tobin
Keyword(s):  
Chick Embryo ◽  
Hemoglobin Synthesis
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