scholarly journals Intestinal transfer of growth hormone into the circulatory system of the rainbow trout,Salmo gairdneri: Interference by granule cells

1989 ◽  
Vol 251 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Pierre-Yves Le Bail ◽  
Marie-France Sire ◽  
Jean-Marie Vernier
Keyword(s):  
Growth Hormone ◽  
Rainbow Trout ◽  
Granule Cells ◽  
Circulatory System ◽  
Salmo Gairdneri

The development of the stratum granulosum of the small intestine of the rainbow trout (Salmo gairdneri)

1982 ◽  
Vol 60 (6) ◽  
pp. 1513-1516 ◽  
Author(s):  
T. Bergeron ◽  
Bill Woodward
Keyword(s):  
Small Intestine ◽  
Rainbow Trout ◽  
Granule Cells ◽  
Critical Role ◽  
Salmo Gairdneri ◽  
Chronological Age ◽  
Stratum Granulosum

The ontogeny of the stratum granulosum within the small intestine of the rainbow trout (Salmo gairdneri) was related to the chronological age of the animal. The component granule cells were first seen many weeks after the development of the collagenous stratum compactum. It is therefore concluded that the granule cells do not perform a critical role in stratum compactum formation. In addition, it is proposed that the stratum granulosum develops because of resistance of the stratum compactum to cellular penetration.

Effects of ergocryptine, prolactin, and growth hormone on survival and ion regulation in rainbow trout, Salmo gairdneri

1978 ◽  
Vol 56 (11) ◽  
pp. 2394-2401 ◽  
Author(s):  
K. J. Brewer ◽  
B. A. McKeown
Keyword(s):  
Growth Hormone ◽  
Rainbow Trout ◽  
Fresh Water ◽  
Sea Water ◽  
Salmo Gairdneri ◽  
Elevated Plasma ◽  
Ion Regulation ◽  
Simultaneous Injection ◽  
Ion Concentrations ◽  
Peripheral Action

Ergocryptine, an ergot alkaloid that suppresses prolactin (PRL) secretion and also affects growth hormone (GH) levels in mammals, was injected into juvenile rainbow trout, Salmo gairdneri, averaging 8 to 31 g. In fresh water the drug caused few fatalities but lowered plasma Na+, plasma Ca2+, muscle K+, muscle Mg2+, and haematocrit values. The simultaneous injection of low doses of PRL or GH did not significantly modify the results obtained with the drug alone except for an increase in muscle Mg2+ levels. This suggests a possible peripheral action of the drug.Ergocryptine caused the death of S. gairdneri in dilute sea water, while in certain circumstances PRL and GH prevented mortalities. In this medium the drug caused elevated levels of plasma Ca2+ and muscle K+. The simultaneous injection of ergocryptine plus PRL in hyperosmotic media produced an elevated plasma Na+ concentration but a decrease in intracellular Na+ content and concentration.The results suggest that PRL is not critical for the survival of S. gairdneri in fresh water but may still be necessary to maintain body ion concentrations. In hyperosmotic media, however, PRL and GH may be essential for survival and may act on general metabolic processes rather than specific osmoregulatory organs.

Rainbow Trout (Salmo gairdneri) Growth Hormone: in vitro Translation of Pituitary RNA and Product Analysis

1986 ◽  
Vol 43 (7) ◽  
pp. 1327-1331 ◽  
Author(s):  
L. B. Agellon ◽  
T. T. Chen ◽  
R. J. Van Beneden ◽  
R. A. Sonstegard ◽  
G. F. Wagner ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Rainbow Trout ◽  
Blot Analysis ◽  
Immunoblot Analysis ◽  
In Vitro Translation ◽  
Salmo Gairdneri ◽  
Western Immunoblot ◽  
Western Immunoblot Analysis ◽  
Rna Blot Analysis

We have used RNA blot analysis, in vitro translation, and protein immunoblot analysis to characterize mRNA in the pituitary glands of rapidly growing rainbow trout (Salmo gairdneri). Cell-free translation products of total RNA, analyzed by direct immunoprecipitation or western immunoblot analysis with an antiserum to chum salmon (Oncorphynchus keta) growth hormone (GH), indicate that rainbow trout pregrowth hormone (pre-GH) is a polypeptide of 25 000 Da. RNA blot analysis of total pituitary RNA to its cDNA, using total liver RNA as a competitor, revealed the presence of at least four size classes of pituitary mRNA sequences. One class, with a size smaller than 18S rRNA, is the predominant mRNA component. By in vitro translation and western immunoblot analysis, the pre-GH mRNA activity is associated with this class of mRNA sequences. These results suggest that rainbow trout pre-GH mRNA is in the same size range as that of mammalian GH mRNA.

Sign in / Sign up

Export Citation Format

Share Document