Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers

2011 ◽  
Vol 83 (9) ◽  
pp. 1522-1529 ◽  
Author(s):  
Livia Melo Villar ◽  
Jaqueline Correia de Oliveira ◽  
Helena Medina Cruz ◽  
Clara Fumiko Tachibana Yoshida ◽  
Elisabeth Lampe ◽  
...  
2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Livia Melo Villar ◽  
Cristianne Sousa Bezerra ◽  
Helena Medina Cruz ◽  
Moyra Machado Portilho ◽  
Geane Lopes Flores

Hepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or high-risk groups. The main objective of this review is to give an insight about the usefulness of oral fluid and DBS for detecting HBV markers. Several groups have evaluated the detection of HBsAg, anti-HBc, and anti-HBs markers in oral fluid and DBS samples demonstrating 13 to 100% of sensitivity and specificity according different groups, sample collectors, and diagnosis assays. In the same way, HBV DNA detection using oral fluid and DBS samples demonstrate different values of sensitivity according type of collection, studied group, extraction, and detection methods. Thus, serological and molecular diagnostic tests demonstrated good performance for detecting HBV using oral fluid and DBS according some characteristics and could be useful to increase the access to the diagnosis of HBV.


PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61077 ◽  
Author(s):  
Sofiane Mohamed ◽  
Audrey Raimondo ◽  
Guillaume Pénaranda ◽  
Claire Camus ◽  
Denis Ouzan ◽  
...  

2012 ◽  
Vol 56 ◽  
pp. S62
Author(s):  
P. Halfon ◽  
A. Raimondo ◽  
D. Ouzan ◽  
M. Bourlière ◽  
H. Khiri ◽  
...  

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Cristianne Sousa Bezerra ◽  
Moyra Machado Portilho ◽  
Cristiane Cunha Frota ◽  
Lívia Melo Villar

2020 ◽  
Vol 277 ◽  
pp. 113798
Author(s):  
Helena Medina Cruz ◽  
Vanessa Salete de Paula ◽  
Juliana Custódio Miguel Cruz ◽  
Kycia Maria Rodrigues do Ó ◽  
Flavio Augusto Pádua Milagres ◽  
...  

1990 ◽  
Vol 49 (4) ◽  
pp. 708-713 ◽  
Author(s):  
PO-MIN CHEN ◽  
SHENG FAN ◽  
CHIA-JUI LIU ◽  
RUEY-KUEN HSIEH ◽  
JIN-HWANG LIU ◽  
...  

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