Disposition and Gene Expression Characteristics in Solid Tumors and Skeletal Muscle after Direct Injection of Naked Plasmid DNA in Mice

2003 ◽  
Vol 92 (6) ◽  
pp. 1295-1304 ◽  
Author(s):  
Atsushi Kawase ◽  
Takehiko Nomura ◽  
Kei Yasuda ◽  
Naoki Kobayashi ◽  
Mitsuru Hashida ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Solid Tumors ◽  
Plasmid Dna ◽  
Direct Injection ◽  
Naked Plasmid ◽  
Expression Characteristics ◽  
Naked Plasmid Dna

Expression of naked plasmids by cultured myotubes and entry of plasmids into T tubules and caveolae of mammalian skeletal muscle

1992 ◽  
Vol 103 (4) ◽  
pp. 1249-1259
Author(s):  
J.A. Wolff ◽  
M.E. Dowty ◽  
S. Jiao ◽  
G. Repetto ◽  
R.K. Berg ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasmid Dna ◽  
Cardiac Cells ◽  
Mammalian Skeletal Muscle ◽  
Cultured Myotubes ◽  
Naked Plasmid ◽  
T Tubules ◽  
Naked Plasmid Dna

Plasmid DNA or artificial mRNA injected intramuscularly into skeletal muscle via a 27 g needle expressed transgenes at relatively efficient levels in skeletal myofibers and cardiac cells. In the present study, several approaches were used to determine the mechanism of cellular uptake. After exposure of naked plasmid DNA, primary rat muscle cells in vitro expressed transgenes to a much greater extent than other types of immortalized or primary cells. In vivo light microscope studies showed that intramuscularly injected plasmid DNA was distributed throughout the muscle and was able to diffuse through the extracellular matrix, cross the external lamina, and enter myofibers. Electron microscope studies showed that colloidal gold conjugated to plasmid DNA traversed the external lamina and entered T tubules and caveolae, while gold complexed with polylysine, polyethylene glycol or polyglutamate primarily remained outside of the myofibers. The results indicate that it is highly unlikely that the plasmid DNA enters the myofiber simply by the needle grossly disrupting the sarcolemma. In addition, transient membrane disruptions do not appear to be responsible for the uptake of DNA. Furthermore, no evidence for endocytosis could be found. The possible uptake of plasmid DNA by some type of cell membrane transporter, in particular via potocytosis, is discussed.

A NOVEL EX VIVO ANGIOGENESIS ASSAY BASED ON ELECTROPORATION-MEDIATED DELIVERY OF NAKED PLASMID DNA TO SKELETAL MUSCLE

2004 ◽  
Vol 13 (3) ◽  
pp. 23
Author(s):  
Hyung-Suk Jang ◽  
Hyun-Joong Kim ◽  
Jeong-Min Kim ◽  
Young-Sam Lee ◽  
Koung Li Kim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasmid Dna ◽  
Ex Vivo ◽  
Angiogenesis Assay ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung

2007 ◽  
Vol 9 (5) ◽  
pp. 369-380 ◽  
Author(s):  
Ian A. Pringle ◽  
Gerry McLachlan ◽  
David D. S. Collie ◽  
Stephanie G. Sumner-Jones ◽  
Anna E. Lawton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Plasmid Dna ◽  
Reporter Gene Expression ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

Uptake and Gene Expression of Naked Plasmid DNA in Cultured Brain Microvessel Endothelial Cells

1998 ◽  
Vol 245 (1) ◽  
pp. 235-239 ◽  
Author(s):  
Masaru Nakamura ◽  
Pablo Davila-Zavala ◽  
Hideaki Tokuda ◽  
Yoshinobu Takakura ◽  
Mitsuru Hashida
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Plasmid Dna ◽  
Naked Plasmid ◽  
Microvessel Endothelial Cells ◽  
Brain Microvessel ◽  
Naked Plasmid Dna

scholarly journals A Novel ex Vivo Angiogenesis Assay Based on Electroporation-Mediated Delivery of Naked Plasmid DNA to Skeletal Muscle

2004 ◽  
Vol 9 (3) ◽  
pp. 464-474 ◽  
Author(s):  
Hyung-Suk Jang ◽  
Hyun-Joong Kim ◽  
Jeong-Min Kim ◽  
Young-Sam Lee ◽  
Koung Li Kim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasmid Dna ◽  
Ex Vivo ◽  
Angiogenesis Assay ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

A comprehensive study of optimal conditions for naked plasmid DNA transfer into skeletal muscle by electroporation

2005 ◽  
Vol 7 (9) ◽  
pp. 1235-1245 ◽  
Author(s):  
Xue-Dong Wang ◽  
Jian-Guo Tang ◽  
Xiao-lei Xie ◽  
Ji-Chun Yang ◽  
Shuai Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Optimal Conditions ◽  
Naked Plasmid ◽  
Naked Plasmid Dna ◽  
Comprehensive Study
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