Optimisation of the in-vivo assay conditions for nitrate reductase in barley (Hordeum vulgare L. cv. Igri)

1984 ◽  
Vol 35 (7) ◽  
pp. 725-730 ◽  
Author(s):  
Gordon J. Sym
Keyword(s):  
Hordeum Vulgare ◽  
Nitrate Reductase ◽  
Hordeum Vulgare L ◽  
In Vivo Assay ◽  
Assay Conditions

In vivo assay of nitrate reductase in tea (Camellia sinensis L.) leaf and root

1987 ◽  
Vol 41 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Tarun Ch. Sarmah ◽  
Mahinath Dev Choudhury ◽  
Monoranjan Goswami
Keyword(s):  
Nitrate Reductase ◽  
Camellia Sinensis ◽  
In Vivo Assay

scholarly journals In vivo and in vitro synthesis of CM-proteins (A-hordeins) from barley (Hordeum vulgare L.)

Planta ◽  
1983 ◽  
Vol 157 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Javier Paz-Ares ◽  
Fernando Ponz ◽  
Cipriano Aragoncillo ◽  
Carlos Hern�ndez-Lucas ◽  
Gabriel Salcedo ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
In Vitro Synthesis

In vivo Nitrate Reductase Activity in Leaves of Pearl Millet, Pennisetum americanum (L.) Leeke

1987 ◽  
Vol 14 (2) ◽  
pp. 125 ◽  
Author(s):  
SV Chanda ◽  
AK Joshi ◽  
PN Krishnan ◽  
YD Singh
Keyword(s):  
Nitrate Reductase ◽  
Reductase Activity ◽  
Potassium Phosphate ◽  
Limiting Factor ◽  
Growth Stages ◽  
In Vivo Assay ◽  
Rate Limiting ◽  
Nr Activity

In the in vivo assay of nitrate reductase (NR) in P. americanum leaves, addition of 1% (v/v) Triton X-100, potassium phosphate buffer (80 mM, pH 7.4) and 1.13 mM NADH to the assay medium resulted in maximum activity. With increasing concentration of NADH, saturation-type kinetics were observed. Based on this data metabolic pool concentration for NADH and apparent Km for nitrate reductase were determined. In field studies with cultivars BJ-104, J-104 and 5141-A of P. americanum, the relative limitation of NO3-, NADH and nitrate reductase in NO3- assimilation was determined. NR activity was measured by four modifications of the in vivo assay technique (with NO3-, with NADH, without NO3- and NADH and with both NO3- and NADH additions to the reaction mixture) and with one in vitro technique. For all the cultivars, NADH was the major rate-limiting factor for in vivo assay during early growth stages, while at later stages, NO3- was limiting. At no stage was NR rate-limiting. It is concluded that NR activity alone may not serve as biochemical marker for improved efficiency of utilisation of nitrogen in P. americanum.

Sign in / Sign up

Export Citation Format

Share Document