Remarkable temporal stability of high-abundance human plasma proteins assessed by targeted mass spectrometry

2012 ◽  
Vol 6 (11-12) ◽  
pp. 626-634 ◽  
Author(s):  
Sarah A. Randall ◽  
Matthew J. McKay ◽  
Dana Pascovici ◽  
Kate Mahon ◽  
Lisa Horvath ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Plasma Proteins ◽  
Temporal Stability ◽  
High Abundance

scholarly journals Preprint: A robust peptidomics mass spectrometry platform for measuring oxytocin in plasma and serum

2016 ◽  
Author(s):  
Ole Kristian Brandtzaeg ◽  
Elin Johnsen ◽  
Hanne Roberg-Larsen ◽  
Knut Fredrik Seip ◽  
Siri Leknes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Plasma Proteins ◽  
High Performance ◽  
Cyclic Peptide ◽  
Liquid Chromatography Mass Spectrometry ◽  
Cord Serum ◽  
Chromatography Mass Spectrometry ◽  
Nano Liquid Chromatography

Current approaches to measuring the cyclic peptide oxytocin in plasma/serum are associated with poor selectivity and/or inadequate sensitivity. We here describe a high performance nano liquid chromatography-mass spectrometry platform for measuring OT in human plasma/serum. The platform is extremely robust, allowing laborious sample clean-up steps to be omitted. OT binds strongly to plasma proteins, but a reduction/alkylation procedure breaks this bond, allowing ample detection of total OT. The method showed excellent quantitation properties, and was used to determine total OT levels to 0.5-1.2 ng/mL (evaluated with human plasma and cord serum). The method is compatible with accessible mass spectrometry instrumentation, finally allowing selective and easily comparable oxytocin measurements.

scholarly journals Investigation of human plasma depletome from patients with schizophrenia

2019 ◽  
Author(s):  
Licia Carla da Silva Costa ◽  
Daniel Martins de Souza ◽  
Sheila Garcia Rosa ◽  
Paulo A. Baldasso ◽  
Johann Steiner
Keyword(s):  
Mass Spectrometry ◽  
Blood Plasma ◽  
Psychiatric Disorders ◽  
Treatment Outcomes ◽  
Human Plasma ◽  
High Abundance ◽  
Improve Treatment ◽  
Dynamic Concentration ◽  
Shotgun Mass Spectrometry ◽  
Potential Biomarkers

The proteome of blood plasma is an interesting source of biomarkers and a potential way to improve treatment outcomes in psychiatric disorders. Respire that, its wide dynamic concentration range makes reducing its complexity necessary. Thus, in proteomic studies, a few of the most abundant proteins are depleted and normally discarded. This high-abundance fraction, called the depletome, however, is a source of potential biomarkers due to nonspecific bindings with low abundance proteins. In this work, we aimed to characterize the high-abundance fraction using a shotgun mass spectrometry approach. These proteins show the importance of studying depletome proteins in the quest for biomarkers.

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