3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks

Small ◽  
2021 ◽  
pp. 2104820
Author(s):  
Fukun He ◽  
Yangteng Ou ◽  
Ji Liu ◽  
Qiu Huang ◽  
Bao Tang ◽  
...  
Keyword(s):  
3D Printed ◽  
Living Materials

scholarly journals Metabolism Control in 3D Printed Living Materials

2021 ◽  
Author(s):  
Tobias Butelmann ◽  
Hans Priks ◽  
Zoel Parent ◽  
Trevor G. Johnston ◽  
Tarmo Tamm ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Terminal Electron Acceptor ◽  
Three Dimensional Printing ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Brewing Process ◽  
3D Printed ◽  
Dimensional Printing ◽  
Living Materials

AbstractThe three-dimensional printing of cells offers an attractive opportunity to design and develop innovative biotechnological applications, such as the fabrication of biosensors or modular bioreactors. Living materials (LMs) are cross-linked polymeric hydrogel matrices containing cells, and recently, one of the most deployed LMs consists of F127-bis-urethane methacrylate (F127-BUM). The material properties of F127-BUM allow reproducible 3D printing and stability of LMs in physiological environments. These materials are permissible for small molecules like glucose and ethanol. However, no information is available for oxygen, which is essential— for example, towards the development of aerobic bioprocesses using microbial cell factories. To address this challenge, we investigated the role of oxygen as a terminal electron acceptor in the budding yeast’s respiratory chain and determined its permissibility in LMs. We quantified the ability of cell-retaining LMs to utilize oxygen and compared it with cells in suspension culture. We found that the cells’ ability to consume oxygen was heavily impaired inside LMs, indicating that the metabolism mostly relied on fermentation instead of respiration. To demonstrate an application of these 3D printed LMs, we evaluated a comparative brewing process. The analysis showed a significantly higher (3.7%) ethanol production using 3D printed LMs than traditional brewing, indicating an efficient control of the metabolism. Towards molecular and systems biology studies using LMs, we developed a highly reliable method to isolate cells from LMs for flow cytometry and further purified macromolecules (proteins, RNA, and DNA). Our results show the application of F127-BUM-based LMs for microaerobic processes and envision the development of diverse bioprocesses using versatile LMs in the future.

Metabolism Control in 3D-Printed Living Materials Improves Fermentation

2021 ◽  
Vol 4 (9) ◽  
pp. 7195-7203
Author(s):  
Tobias Butelmann ◽  
Hans Priks ◽  
Zoel Parent ◽  
Trevor G. Johnston ◽  
Tarmo Tamm ◽  
...  
Keyword(s):  
3D Printed ◽  
Living Materials

3D Printed Models for Surgical Planning and Reconstructive Implant Design in Sphenoorbital Tumor Surgery

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Hassan Othman ◽  
Sam Evans ◽  
Daniel Morris ◽  
Saty Bhatia ◽  
Caroline Hayhurst
Keyword(s):  
Surgical Planning ◽  
Implant Design ◽  
Tumor Surgery ◽  
3D Printed

That which Is Unseen: A 3D-Printed Case-Based Didactic Approach to Teaching Pediatric Cerebrovascular Neurosurgery

2019 ◽  
Author(s):  
Avital Perry ◽  
Soliman Oushy ◽  
Lucas Carlstrom ◽  
Christopher Graffeo ◽  
David Daniels ◽  
...  
Keyword(s):  
3D Printed ◽  
Approach To Teaching ◽  
Case Based

The Effect of Surface Texture on Color Appearance of 3D Printed Objects

2018 ◽  
Vol 2018 (1) ◽  
pp. 128-133
Author(s):  
Matt Ronnenberg ◽  
Susan Farnand
Keyword(s):  
Surface Texture ◽  
Color Appearance ◽  
3D Printed ◽  
Effect Of Surface

Bioresorption and biodegradation of the 3D-printed gene-activated bone substitutes based on octacalcium phosphate

2020 ◽  
Vol XV (1) ◽  
Author(s):  
E. Presnyakov ◽  
I. Bozo ◽  
I. Smirnov ◽  
V. Komlev ◽  
V. Popov ◽  
...  
Keyword(s):  
Bone Substitutes ◽  
Octacalcium Phosphate ◽  
3D Printed
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