Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications

2013 ◽  
Vol 10 (10) ◽  
pp. E327-E336 ◽  
Author(s):  
Alexander C. Allori ◽  
Edward H. Davidson ◽  
Derek D. Reformat ◽  
Alexander M. Sailon ◽  
James Freeman ◽  
...  
Author(s):  
Towseef Amin Rafeeqi

Carbon-based nanotechnology has been rapidly developing, with a particular interest in the bio-application of carbon nanotubes (CNTs) as a scaffold in tissue engineering. It is essential that the materials used in scaffold fabrication are compatible with cells, as well as with the biological milieu. Many synthetic polymers have been used for tissue engineering so far; however, many lack the necessary mechanical strength and may not be easily functionalized, in contrast to CNTs, which have shown very attractive features as a scaffold for cell culture system. In spite of many attractive features, the toxicity of CNTs is a prime concern. The potential applications of CNTs seem countless, although few have reached a marketable status so far and there is need of more studies on CNTs biocompatibility issues. This chapter aims to revisit the basics of CNTs with their bio-applications including their use as a scaffold in cell culture systems.


ASAIO Journal ◽  
2004 ◽  
Vol 50 (5) ◽  
pp. 464-467 ◽  
Author(s):  
SooWon Suh ◽  
Jinhoon Kim ◽  
JiYoun Shin ◽  
Kwanghyun Kil ◽  
Kwahnmien Kim ◽  
...  

1988 ◽  
Vol 7 (3) ◽  
pp. 217-227 ◽  
Author(s):  
Felix K. Gmünder ◽  
Claudy-Gabrielle Nordau ◽  
Alexander Tschopp ◽  
Beat Huber ◽  
Augusto Cogoli

2014 ◽  
Vol 5 ◽  
pp. 204173141454396 ◽  
Author(s):  
Roman A Perez ◽  
Kiara Riccardi ◽  
George Altankov ◽  
Maria-Pau Ginebra

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.


2018 ◽  
Vol 257 ◽  
pp. 650-657 ◽  
Author(s):  
Azadeh Nilghaz ◽  
Siew Hoo ◽  
Wei Shen ◽  
Xiaonan Lu ◽  
Peggy P.Y. Chan

2015 ◽  
Vol 120 ◽  
pp. 279-285 ◽  
Author(s):  
Sabine Schnell ◽  
Kafilat Bawa-Allah ◽  
Adebayo Otitoloju ◽  
Christer Hogstrand ◽  
Thomas H. Miller ◽  
...  

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  

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