Histochemical Localization of a Chimeric Gene (rolC-GUS) Expression in Zygotic Embryos of Transgenic Tobacco Plants

1994 ◽  
Vol 73 (5) ◽  
pp. 465-469
Author(s):  
E Aspuria
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Chimeric Gene ◽  
Histochemical Localization ◽  
Zygotic Embryos ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Gene Rolc

scholarly journals Expression Pattern of Arabidopsis Thaliana Pollen- and Embryo-Specific Promoter in Transgenic Tobacco Plants

2014 ◽  
Vol 56 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Martin Jopcik ◽  
Ildiko Matusikova ◽  
Jana Moravcikova ◽  
Jana Libantova
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Tobacco ◽  
Expression Profiles ◽  
Pollen Grains ◽  
Apical Part ◽  
Gus Expression ◽  
Transgenic Tobacco Plants ◽  
Specific Expression ◽  
Tobacco Plants ◽  
Biotechnology Research

Abstract Current biotechnology research is focused on tissue-specific expression of genes of interest in plants. Promoters with specific spatial and temporal expression profiles in targeted organisms are in wide use for this. This study investigated whether the Arabidopsis thaliana seed- and pollen-specific promoter MXL maintains its specificity in transgenic tobacco plants. Histochemical analysis revealed that the MXL fusion promoter drives slightly different GUS expression in that heterologous organism. GUS staining was clearly detected in the bicellular stage of pollen development and later in germinating tobacco pollen grains. Unlike in A. thaliana, where the MXL promoter is active during the whole period of embryo development, in tobacco its activity was restricted to a short temporal and spatial window from late-heart to mid-torpedo stages, mainly in the apical part of the developing embryo. These results point to the need to test the expression profiles of heterologous promoters in targeted species before they are used in particular biotechnological programs.

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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