Cloning, Sequencing, and Characterization of Ribosomal Protein and RNA Polymerase Genes from the Region Analogous to the α-Operon of Escherichia coli in Halophilic Archaea, Halobacterium halobium

1999 ◽  
Vol 264 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Kazuyoshi Sano ◽  
Atsuo Taguchi ◽  
Hiromi Furumoto ◽  
Taizo Uda ◽  
Takuzi Itoh
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Protein ◽  
Halophilic Archaea ◽  
Halobacterium Halobium

The relationship between the spoT gene, the synthesis of stable RNA, ribosomal proteins, and the ββ′ subunits of RNA polymerase following a nutritional shiftup of Escherichia coli

1978 ◽  
Vol 56 (6) ◽  
pp. 528-533 ◽  
Author(s):  
Stephen M. Boyle ◽  
Frederick Chu ◽  
Nathan Brot ◽  
Bruce H. Sells
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Rna Synthesis ◽  
Transient Increase ◽  
Spot Gene ◽  
The Relationship

The level of ppGpp and rates of synthesis of stable RNA, ribosomal protein, and the β and β′ subunits of RNA polymerase were measured following a nutritional shiftup in Escherichia coli strains, NF 929 (spoT+) and NF 930 (spoT'−). In the spoT+ strain, ppGpp levels decreased 50% within 2 min following shiftup, and the rates of synthesis of stable RNA, ribosomal proteins, and the β and β′ subunits of RNA polymerase increased with little or no lag. In contrast, in the spoT− strain, ppGpp levels transiently increased 40% during the first 6 min following shiftup. An inhibition in the rate of stable RNA synthesis and a delay in the increased synthesis of ribosomal proteins and β and β′ subunits occurred concurrently with the transient increase in ppGpp. In addition, the DNA-dependent synthesis in vitro of the β and β′ subunits of RNA polymerase was inhibited by physiological levels of ppGpp. Because of the timing and magnitude of the changes in ppGpp levels in the spoT− strain versus the timing when the new rates of stable RNA, ribosomal protein, and β and β′ subunits synthesis are reached, it is concluded that ppGpp is not the sole element regulating the expression of these genes.

Precise Characterization of the Epitope Recognized by a Monoclonal Antibody Against Escherichia coli RNA Polymerase

Hybridoma ◽  
2005 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Estelle Andre ◽  
Martine Pugniere ◽  
Jaqueline Latouche ◽  
Claude Granier ◽  
Jean-Paul Leonetti
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibody ◽  
Rna Polymerase ◽  
Precise Characterization

Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids

1979 ◽  
Vol 173 (1) ◽  
pp. 39-50 ◽  
Author(s):  
N. P. Fiil ◽  
D. Bendiak ◽  
J. Collins ◽  
J. D. Friesen
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribosomal Protein
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