DIFFERENTIAL REGULATION BY IL-4 AND IL-10 OF RADIATION-INDUCED IL-6 AND IL-8 PRODUCTION AND ICAM-1 EXPRESSION BY HUMAN ENDOTHELIAL CELLS

Cytokine ◽  
1999 ◽  
Vol 11 (11) ◽  
pp. 831-838 ◽  
Author(s):  
Anne Van Der Meeren ◽  
Claire Squiban ◽  
Patrick Gourmelon ◽  
Hélène Lafont ◽  
Marie-Hélène Gaugler
Keyword(s):  
Endothelial Cells ◽  
Differential Regulation ◽  
Human Endothelial Cells ◽  
Radiation Induced

scholarly journals GDF15 contributes to radiation-induced senescence through the ROS-mediated p16 pathway in human endothelial cells

Oncotarget ◽  
2016 ◽  
Vol 7 (9) ◽  
pp. 9634-9644 ◽  
Author(s):  
Hyejin Park ◽  
Chun-Ho Kim ◽  
Jae-Hoon Jeong ◽  
Myungjin Park ◽  
Kwang Seok Kim
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Radiation Induced

scholarly journals Global Transcriptional Profiling Reveals Novel Autocrine Functions of Interleukin 6 in Human Vascular Endothelial Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Liza U. Ljungberg ◽  
Mulugeta M. Zegeye ◽  
Caroline Kardeby ◽  
Knut Fälker ◽  
Dirk Repsilber ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
Pathway Analysis ◽  
Interleukin 6 ◽  
Vascular Endothelial Cells ◽  
Differential Regulation ◽  
Vascular Endothelial ◽  
Human Endothelial Cells ◽  
Cellular Functions ◽  
Cellular Movement

Background. Interleukin 6 (IL6) is a multifunctional cytokine produced by various cells, including vascular endothelial cells. IL6 has both pro- and non-/anti-inflammatory functions, and the response to IL6 is dependent on whether it acts via the membrane-bound IL6 receptor α (IL6Rα) (classic signaling) or the soluble form of the receptor (transsignaling). As human endothelial cells produce IL6 and at the same time express IL6Rα, we hypothesized that IL6 may have autocrine functions. Methods. Knockdown of IL6 in cultured human endothelial cells was performed using siRNA. Knockdown efficiency was evaluated using ELISA. RNA sequencing was employed to characterize the transcriptional consequence of IL6 knockdown, and Ingenuity Pathway Analysis was used to further explore the functional roles of IL6. Results. Knockdown of IL6 in cultured endothelial cells resulted in a 84-92% reduction in the release of IL6. Knockdown of IL6 resulted in dramatic changes in transcriptional pattern; knockdown of IL6 in the absence of soluble IL6Rα (sIL6Rα) led to differential regulation of 1915 genes, and knockdown of IL6 in the presence of sIL6Rα led to differential regulation of 1967 genes (fold change 1.5, false discovery rate<0.05). Pathway analysis revealed that the autocrine functions of IL6 in human endothelial cells are mainly related to basal cellular functions such as regulation of cell cycle, signaling, and cellular movement. Furthermore, we found that knockdown of IL6 activates functions related to adhesion, binding, and interaction of endothelial cells, which seem to be mediated mainly via STAT3. Conclusion. In this study, a large number of novel genes that are under autocrine regulation by IL6 in human endothelial cells were identified. Overall, our data indicate that IL6 acts in an autocrine manner to regulate basal cellular functions, such as cell cycle regulation, signaling, and cellular movement, and suggests that the autocrine functions of IL6 in human endothelial cells are mediated via IL6 classic signaling.

Radiation-induced biochemical changes of human endothelial cells

1992 ◽  
Vol 65 ◽  
pp. S87
Author(s):  
M. Verheij ◽  
L. Dewit ◽  
L. Arisz ◽  
G.C.M. Koomen ◽  
J.A. van Mourik
Keyword(s):  
Endothelial Cells ◽  
Biochemical Changes ◽  
Human Endothelial Cells ◽  
Radiation Induced

Protective effects of tetrahydropalmatine against γ-radiation induced damage to human endothelial cells

Life Sciences ◽  
2010 ◽  
Vol 87 (1-2) ◽  
pp. 55-63 ◽  
Author(s):  
Jing Yu ◽  
Bin-kui Piao ◽  
Ying-xia Pei ◽  
Xin Qi ◽  
Bao-jin Hua
Keyword(s):  
Endothelial Cells ◽  
Protective Effects ◽  
Human Endothelial Cells ◽  
Γ Radiation ◽  
Radiation Induced

Radiation-Induced Inhibition of Human Endothelial Cells Replicating in Culture

1976 ◽  
Vol 68 (2) ◽  
pp. 244 ◽  
Author(s):  
R. L. DeGowin ◽  
L. J. Lewis ◽  
R. E. Mason ◽  
M. K. Borke ◽  
J. C. Hoak
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Radiation Induced

Radiation-induced endothelin production by human endothelial cells in vitro

1992 ◽  
Vol 24 ◽  
pp. 275 ◽  
Author(s):  
George Shenouda ◽  
Magdy Labateya ◽  
Luis Souhami ◽  
Ervin B. Podgorsak
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
Radiation Induced

INFLUENCE OF BACTERIAL ENDOTOXIN ON RADIATION-INDUCED ACTIVATION OF HUMAN ENDOTHELIAL CELLS IN VITRO AND IN VIVO

1997 ◽  
Vol 64 (9) ◽  
pp. 1370-1373 ◽  
Author(s):  
Heidrun Lindner ◽  
Ernst Holler ◽  
Armin Gerbitz ◽  
Judith P. Johnson ◽  
Georg W. Bornkamm ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Bacterial Endotoxin ◽  
Human Endothelial Cells ◽  
Radiation Induced
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