Predictive model for growth of Clostridium perfringens at temperatures applicable to cooling of cooked meat

1999 ◽  
Vol 16 (4) ◽  
pp. 335-349 ◽  
Author(s):  
V.K Juneja ◽  
R.C Whiting ◽  
H.M Marks ◽  
O.P Snyder
Keyword(s):  
Predictive Model ◽  
Clostridium Perfringens ◽  
Cooked Meat

Predictive model for growth of Clostridium perfringens during cooling of cooked pork supplemented with sodium chloride and sodium pyrophosphate

Meat Science ◽  
2021 ◽  
pp. 108557
Author(s):  
Vijay K. Juneja ◽  
Marangeli Osoria ◽  
Anuj S. Purohit ◽  
Chase E. Golden ◽  
Abhinav Mishra ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Predictive Model ◽  
Clostridium Perfringens ◽  
Sodium Pyrophosphate

scholarly journals Incidence and contamination level of Clostridium perfringens in meat and meat products sold in Sakarya province of Turkey

2021 ◽  
Vol 7 (3) ◽  
pp. 172-178
Author(s):  
Serap Coşansu ◽  
Şeyma Şeniz Ersöz
Keyword(s):  
Clostridium Perfringens ◽  
Meat Product ◽  
Meat Products ◽  
Ground Beef ◽  
Chicken Meat ◽  
Contamination Level ◽  
Biochemical Tests ◽  
Contamination Levels ◽  
Emulsified Meat ◽  
Cooked Meat

Totally 101 meat and meat product samples obtained from local markets and restaurants were analyzed for incidence and contamination level of Clostridium perfringens. The typical colonies grown anaerobically on Tryptose Sulfite Cycloserine Agar supplemented with 4-Methyliumbelliferyl (MUP) were confirmed by biochemical tests. Forty-eight of the samples (47.5%) were contaminated with C. perfringens. The highest incidence of the pathogen was determined in uncooked meatball samples (72.2%) followed by ground beef samples (61.3%). The incidence of C. perfringens in chicken meat, cooked meat döner, cooked chicken döner and emulsified meat product samples were 33.3, 33.3, 28.6 and 16.7%, respectively. Thirteen out of 101 samples (12.9%) yielded typical colonies on TSC-MUP Agar, but could not be confirmed as C. perfringens. Average contamination levels in sample groups ranged from 8.3 to 1.5×102 cfu/g, with the highest ground beef and the lowest chicken meat.

Structural changes in cells of Clostridium perfringens infected with a short-tailed bacteriophage

1971 ◽  
Vol 17 (3) ◽  
pp. 397-402 ◽  
Author(s):  
David E. Bradley ◽  
Judith F. M. Hoeniger
Keyword(s):  
Electron Microscope ◽  
Clostridium Perfringens ◽  
Structural Changes ◽  
Cell Envelope ◽  
Cell Lysis ◽  
Cell Periphery ◽  
Normal Cells ◽  
Phage Growth ◽  
Cooked Meat ◽  
In Cells

A culture of Clostridium perfringens strain 80 in cooked meat broth contained three types of cell: normal, long, and small round cells. The process of phage maturation and cell lysis was studied in all three forms using the electron microscope. In normal cells, the nucleoplasm first enlarged, then small clear areas, slightly larger than intracellular phages, appeared mainly around the cell periphery. The nucleoplasm dispersed and mature phage particles were formed. Subsequent lysis was caused by the breaking off of portions of the cell envelope leaving holes for the release of the contents. Long cells were also able to support phage growth.

Impact of Cooking, Cooling, and Subsequent Refrigeration on the Growth or Survival of Clostridium perfringens in Cooked Meat and Poultry Products

2003 ◽  
Vol 66 (7) ◽  
pp. 1227-1232 ◽  
Author(s):  
ROBIN M. KALINOWSKI ◽  
R. BRUCE TOMPKIN ◽  
PETER W. BODNARUK ◽  
W. PAYTON PRUETT
Keyword(s):  
Public Health ◽  
Food Safety ◽  
Health Risk ◽  
Clostridium Perfringens ◽  
Meat Products ◽  
Performance Standards ◽  
Public Health Risk ◽  
Raw Meat ◽  
Poultry Products ◽  
Cooked Meat

In January 1999, the Food Safety and Inspection Service (FSIS) finalized performance standards for the cooking and chilling of meat and poultry products in federally inspected establishments. More restrictive chilling (stabilization)requirements were adopted despite the lack of strong evidence of a public health risk posed by industry practices employing the original May 1988 guidelines (U.S. Department of Agriculture FSIS Directive 7110.3). Baseline data led the FSIS to estimate a “worst case” of 104 Clostridium perfringens cells per g in raw meat products. The rationale for the FSIS performance standards was based on this estimate and the assumption that the numbers detected in the baseline study were spores that could survive cooking. The assumptions underlying the regulation stimulated work in our laboratory to help address why there have been so few documented outbreaks of C. perfringens illness associated with the consumption of commercially processed cooked meat and poultry products. Our research took into account the numbers of C. perfringens spores in both raw and cooked products. One hundred ninety-seven raw comminuted meat samples were cooked to 73.9°C and analyzed for C. perfringens levels. All but two samples had undetectable levels (<3 spores per g). Two ground pork samples contained 3.3 and 66 spores per g. Research was also conducted to determine the effect of chilling on the outgrowth of C. perfringens spores in cured and uncured turkey. Raw meat blends inoculated with C. perfringens spores, cooked to 73.9°C, and chilled according to current guidelines or under abuse conditions yielded increases of 2.25 and 2.44 log10 CFU/g for uncured turkey chilled for 6 h and an increase of 3.07 log10 CFU/g for cured turkey chilled for 24 h. No growth occurred in cured turkey during a 6-h cooling period. Furthermore, the fate of C. perfringens in cooked cured and uncured turkey held at refrigeration temperatures was investigated. C. perfringens levels decreased by 2.52, 2.54, and 2.75 log10 CFU/g in cured turkey held at 0.6, 4.4, and 10°C, respectively, for 7 days. Finally, 48 production lots of ready-to-eat meat products that had deviated from FSIS guidelines were analyzed for C. perfringens levels. To date, 456 samples have been tested, and all but 25 (ranging from 100 to 710 CFU/g) of the samples contained C. perfringens at levels of <100 CFU/g. These results further support historical food safety data that suggest a very low public health risk associated with C. perfringens in commercially processed ready-to-eat meat and poultry products.

Predictive Model for Clostridium perfringens Growth in Roast Beef during Cooling and Inhibition of Spore Germination and Outgrowth by Organic Acid Salts†‡

2005 ◽  
Vol 68 (12) ◽  
pp. 2594-2605 ◽  
Author(s):  
MARCOS X. SÁNCHEZ-PLATA ◽  
ALEJANDRO AMÉZQUITA ◽  
ERIN BLANKENSHIP ◽  
DENNIS E. BURSON ◽  
VIJAY JUNEJA ◽  
...  
Keyword(s):  
Organic Acid ◽  
Predictive Model ◽  
Clostridium Perfringens ◽  
Spore Germination ◽  
Sodium Citrate ◽  
Sodium Lactate ◽  
Processed Meat ◽  
Roast Beef ◽  
Organic Acid Salts ◽  
Acid Salts

Spores of foodborne pathogens can survive traditional thermal processing schedules used in the manufacturing of processed meat products. Heat-activated spores can germinate and grow to hazardous levels when these products are improperly chilled. Germination and outgrowth of Clostridium perfringens spores in roast beef during chilling was studied following simulated cooling schedules normally used in the processed-meat industry. Inhibitory effects of organic acid salts on germination and outgrowth of C. perfringens spores during chilling and the survival of vegetative cells and spores under abusive refrigerated storage was also evaluated. Beef top rounds were formulated to contain a marinade (finished product concentrations: 1% salt, 0.2% potassium tetrapyrophosphate, and 0.2% starch) and then ground and mixed with antimicrobials (sodium lactate and sodium lactate plus 2.5% sodium diacetate and buffered sodium citrate and buffered sodium citrate plus 1.3% sodium diacetate). The ground product was inoculated with a three-strain cocktail of C. perfringens spores (NCTC 8238, NCTC 8239, and ATCC 10388), mixed, vacuum packaged, heat shocked for 20 min at 75°C, and chilled exponentially from 54.5 to 7.2°C in 9, 12, 15, 18, or 21 h. C. perfringens populations (total and spore) were enumerated after heat shock, during chilling, and during storage for up to 60 days at 10°C using tryptose-sulfite-cycloserine agar. C. perfringens spores were able to germinate and grow in roast beef (control, without any antimicrobials) from an initial population of ca. 3.1 log CFU/g by 2.00, 3.44, 4.04, 4.86, and 5.72 log CFU/g after 9, 12, 15, 18, and 21 h of exponential chilling. A predictive model was developed to describe sigmoidal C. perfringens growth curves during cooling of roast beef from 54.5 to 7.2°C within 9, 12, 15, 18, and 21 h. Addition of antimicrobials prevented germination and outgrowth of C. perfringens regardless of the chill times. C. perfringens spores could be recovered from samples containing organic acid salts that were stored up to 60 days at 10°C. Extension of chilling time to ≥9 h resulted in >1 log CFU/g growth of C. perfringens under anaerobic conditions in roast beef. Organic acid salts inhibited outgrowth of C. perfringens spores during chilling of roast beef when extended chill rates were followed. Although C. perfringens spore germination is inhibited by the antimicrobials, this inhibition may represent a hazard when such products are incorporated into new products, such as soups and chili, that do not contain these antimicrobials, thus allowing spore germination and outgrowth under conditions of temperature abuse.

INHIBITION OF CLOSTRIDIUM PERFRINGENS BY STREPTOCOCCUS FAECALIS IN A MEDIUM CONTAINING CURING SALTS1

1974 ◽  
Vol 37 (7) ◽  
pp. 387-391
Author(s):  
L. B. Tabatabai ◽  
H. W. Walker
Keyword(s):  
Lactic Acid ◽  
Clostridium Perfringens ◽  
Growth Curve ◽  
Inhibitory Effect ◽  
Inhibitory Effects ◽  
Streptococcus Faecalis ◽  
Inhibition Zones ◽  
Cooked Meat

Inhibition of two strains of Clostridium perfringens by Streptococcus faecalis in Cooked Meat Medium containing curing salts was investigated. Inhibitory effects were evaluated by growth-curve studies and by measurement of inhibition zones on agar. Both strains of C. perfringens were inhibited by S. faecalis in a medium containing glucose, although to different degrees. Addition of nitrite to the medium containing glucose increased the inhibitory effect of S. faecalis towards C. perfringens. Production of lactic acid by S. faecalis appears to play an important role in this inhibition of C. perfringens.

USE OF TIME-TEMPERATURE EVALUATIONS IN DETECTING THE RESPONSIBLE VEHICLE AND CONTRIBUTING FACTORS OF FOODBORNE DISEASE OUTBREAKS1

1971 ◽  
Vol 34 (12) ◽  
pp. 576-582 ◽  
Author(s):  
Frank L. Bryan ◽  
Thomas W. McKinley ◽  
Byron Mixon
Keyword(s):  
Clostridium Perfringens ◽  
Bacterial Growth ◽  
Room Temperature ◽  
Foodborne Illness ◽  
Boiling Water ◽  
Foodborne Disease ◽  
Contributing Factors ◽  
Vegetative Cells ◽  
Use Of Time ◽  
Cooked Meat

An investigation of an outbreak of Clostridium perfringens foodborne illness indicated that turkey or dressing prepared in a school kitchen was responsible. When turkey was again prepared in the kitchen, a bacteriological survey and a time-temperature evaluation were made of the thawing, cooking, chilling, and reheating to which the turkey, stock, or dressing were subjected. During thawing of 22-lb. turkeys in plastic wrappers and in paper bags at room temperature for 18 hr, neither internal nor surface temperatures reached a level at which C. perfringens could grow. Cooking the turkeys in a steamer or in a pot of boiling water raised internal temperatures to a level lethal to vegetative cells. The stock (in gallon jars and a large rectangular pan) and deboned meat (in similar pan) were stored overnight in a reach-in refrigerator. During storage the temperature of both were within a range so that C. perfringens spores could germinate and its vegetative cells multiply for 7–9 hr. The stock was later used in dressing, which when baked, reached internal temperatures known to destroy vegetative cells of C. perfringens. Meat and gravy, when reheated, did not reach such levels. Clostridium perfringens, Staphylocoocus aureus, and Salmonella were isolated from raw turkey; C. perfringens was isolated from cooked meat, stock, and kitchen equipment. Nine recommendations for heat destruction of vegetative cells, inhibition of bacterial growth during storage, and cleaning and sanitizing equipment are made. These recommendations will help prevent foodborne illness whenever turkey and dressing are prepared.

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