Investigation of DP IV-dependent Protein-Protein Interactions using Surface Plasmon Resonance

2005 ◽  
pp. 115-119 ◽  
Author(s):  
Joerg Stork ◽  
Torsten Hoffmann ◽  
Hans-Ulrich Demuth
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Protein Protein Interactions ◽  
Dependent Protein

scholarly journals Surface plasmon resonance: a useful technique for cell biologists to characterize biomolecular interactions

2013 ◽  
Vol 24 (7) ◽  
pp. 883-886 ◽  
Author(s):  
Robert V. Stahelin
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Dissociation Rate ◽  
Biomolecular Interactions ◽  
Protein Protein Interactions ◽  
Interaction Kinetics ◽  
Lipid Protein Interactions ◽  
Dissociation Rate Constants

Surface plasmon resonance (SPR) is a powerful technique for monitoring the affinity and selectivity of biomolecular interactions. SPR allows for analysis of association and dissociation rate constants and modeling of biomolecular interaction kinetics, as well as for equilibrium binding analysis and ligand specificity studies. SPR has received much use and improved precision in classifying protein–protein interactions, as well as in studying small-molecule ligand binding to receptors; however, lipid–protein interactions have been underserved in this regard. With the field of lipids perhaps the next frontier in cellular research, SPR is a highly advantageous technique for cell biologists, as newly identified proteins that associate with cellular membranes can be screened rapidly and robustly for lipid specificity and membrane affinity. This technical perspective discusses the conditions needed to achieve success with lipid–protein interactions and highlights the unique lipid–protein interaction mechanisms that have been elucidated using SPR. It is intended to provide the reader a framework for quantitative and confident conclusions from SPR analysis of lipid–protein interactions.

Identification of protein-protein interactions using Protein Microarrays and Surface Plasmon Resonance Measurements

2005 ◽  
Vol 2005 (Fall) ◽  
Author(s):  
Harald Seitz ◽  
Claus Hultschig ◽  
Holger Eickhoff ◽  
Carsten Zeilinger ◽  
Ulrike Borgmeier ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Protein Microarrays ◽  
Protein Protein Interactions

Identification of Protein-Protein Interactions by Surface Plasmon Resonance followed by Mass Spectrometry

2011 ◽  
pp. 19.21.1-19.21.9 ◽  
Author(s):  
Alexandra Madeira ◽  
Elisabet Vikeved ◽  
Anna Nilsson ◽  
Benita Sjögren ◽  
Per E. Andrén ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Protein Protein Interactions
Sign in / Sign up

Export Citation Format

Share Document