Science ◽  
1965 ◽  
Vol 147 (3659) ◽  
pp. 756-758 ◽  
Author(s):  
W. Halperin ◽  
D. F. Wetherell

1985 ◽  
Vol 55 (3) ◽  
pp. 331-336 ◽  
Author(s):  
M. N. SILVA STORT ◽  
E. A. DOS SANTOS PAVANELLI
Keyword(s):  

HortScience ◽  
1993 ◽  
Vol 28 (6) ◽  
pp. 672-673 ◽  
Author(s):  
S.A. Merkle ◽  
B.A. Watson-Pauley

Bigleaf magnolia (Magnolia macrophylla Michx.) cultures were initiated from immature seeds on an induction medium containing 9.0 μm 2,4-D, 1.1μm BA, and 1 g casein hydrolysate/liter. After 2 months on induction medium, one culture produced adventive embryos. Clumps of embryos transferred to liquid induction medium proliferated as nodules, which grew in diameter, but failed to produce embryos while maintained in induction medium. Nodules transferred to basal medium produced clumps of somatic embryos, which continued to produce repetitive embryos with monthly transfer to fresh basal medium. Individual embryos transferred to basal medium lacking casein hydrolysate germinated and leaves expanded. Plantlets derived from these embryos were transferred to potting mix and acclimatized to greenhouse conditions. Chemical names used: (2,4-dichlorophenoxy)acetic acid (2,4-D); N -(phenylmethyl)-lH-purin-6-amine (BA).


2001 ◽  
Vol 67 (2) ◽  
pp. 352-354 ◽  
Author(s):  
Goro Yoshida ◽  
Koji Yoshikawa ◽  
Toshinobu Terawaki

1969 ◽  
Vol 47 (11) ◽  
pp. 1643-1649 ◽  
Author(s):  
R. Gary Fulcher ◽  
Margaret E. McCully

Regeneration in Fucus vesiculosus L., both under natural conditions and in culture, has been studied histologically.Cut segments of vegetative tissue from mature Fucus specimens were cultured in seawater. Histological sections of this material were prepared at various intervals up to 8 months of culture to follow the development of adventitious branches from the wound surfaces. In both cultured segments and whole, naturally wounded plants, existing filament and cortical cells in the midrib region of wound surfaces differentiate a new epidermis. Several loci on this new epidermis undergo increased rates of periclinal division to produce structures which are morphologically very similar to normal Fucus embryos and which ultimately acquire apical grooves and apical cells and become flattened in the manner of normal vegetative thallus branches. The significance of the wound-healing process and the formation of adventive embryos is discussed.


1972 ◽  
Vol 50 (9) ◽  
pp. 1841-1844 ◽  
Author(s):  
J. McLachlan ◽  
L. C.-M. Chen

In culture adventive embryos were initiated from secondary rhizoidal filaments of sporelings of Fucus distichus subsp. distichus, F. edentatus, F. serratus, and F. vesiculosus. This was not a response to wounding, but seemed to appear spontaneously under various conditions of temperature, light, and nutrition. Only in subsp. distichus did the parent sporeling and its embryos grow significantly, and thereby contribute to the bushy habit of this species. Attempts to grow detached rhizoidal filaments in culture were unsuccessful, but excision of filaments stimulated formation of adventive embryos.


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