Abstract
Based on the whole genome data information of quinoa, the CqSRS gene family members were systematically identified and analyzed by bioinformatics methods, and the responses of CqSRS genes to NaCl (200 mmol/L), SA (200 umol/L) and low temperature (4℃) were detected by qRT-PCR. The results showed that a total of 10 SRS genes were identified in quinoa, and they were distributed on 9 chromosomes, and there were 4 pairs of duplicated genes. The number of amino acids encoded ranged from 143 to 370, and the isoelectric point ranged from 4.81 to 8.90. The secondary structure was mainly composed of random coil(Cc). Most of the CqSRS genes were located in the cytoplasm (5 CqSRS). Phylogenetic analysis showed that the CqSRS gene was divided into three evolutionary groups, and the gene structure showed that the number of exons of CqSRS was between 2–5. Promoter analysis revealed that there are a total of 44 elements related to plant hormone response elements, light response elements, stress response elements and tissue-specific expression in the upstream of the gene. Protein interaction showed that all 10 CqSRS proteins appeared in the known protein interaction network diagram in Arabidopsis. Expression profile analysis showed that CqSRS genes had different expression patterns, and some genes had tissue-specific expression. qRT-PCR showed that all SRS family genes responded to SA, NaCl and low-temperature treatments, but the expression levels of different CqSRS genes were significantly different under various stresses. This study lays a foundation for further analyzed the function of CqSRS family genes.
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue-specific expression of the SBP2 promoter