Cloning of Plant-Virus Genomes Other than that of Cauliflower Mosaic Virus

1985 ◽  
pp. 277-292
Author(s):  
Léon Hirth
Keyword(s):  
Mosaic Virus ◽  
Plant Virus ◽  
Cauliflower Mosaic Virus ◽  
Virus Genomes

scholarly journals Recombination in a plant virus: template-switching in cauliflower mosaic virus

1986 ◽  
Vol 5 (4) ◽  
pp. 641-646 ◽  
Author(s):  
Nigel Grimsley ◽  
Thomas Hohn ◽  
Barbara Hohn
Keyword(s):  
Mosaic Virus ◽  
Plant Virus ◽  
Cauliflower Mosaic Virus ◽  
Template Switching

scholarly journals Arabidopsis thaliana as a model for the study of plant–virus co-evolution

2010 ◽  
Vol 365 (1548) ◽  
pp. 1983-1995 ◽  
Author(s):  
Israel Pagán ◽  
Aurora Fraile ◽  
Elena Fernandez-Fueyo ◽  
Nuria Montes ◽  
Carlos Alonso-Blanco ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Plant Virus ◽  
Cauliflower Mosaic Virus ◽  
Growth Stages ◽  
Virus Species ◽  
Life Styles ◽  
Two Factors ◽  
Virus Genotypes

Understanding plant–virus coevolution requires wild systems in which there is no human manipulation of either host or virus. To develop such a system, we analysed virus infection in six wild populations of Arabidopsis thaliana in Central Spain. The incidence of five virus species with different life-styles was monitored during four years, and this was analysed in relation to the demography of the host populations. Total virus incidence reached 70 per cent, which suggests a role of virus infection in the population structure and dynamics of the host, under the assumption of a host fitness cost caused by the infection. Maximum incidence occurred at early growth stages, and co-infection with different viruses was frequent, two factors often resulting in increased virulence. Experimental infections under controlled conditions with two isolates of the most prevalent viruses, cauliflower mosaic virus and cucumber mosaic virus, showed that there is genetic variation for virus accumulation, although this depended on the interaction between host and virus genotypes. Comparison of Q ST -based genetic differentiations between both host populations with F ST genetic differentiation based on putatively neutral markers suggests different selection dynamics for resistance against different virus species or genotypes. Together, these results are compatible with a hypothesis of plant–virus coevolution.

The spherically averaged structure of a DNA isometric plant virus: Cauliflower mosaic virus

Virology ◽  
1987 ◽  
Vol 159 (1) ◽  
pp. 166-168 ◽  
Author(s):  
J. Kruse ◽  
P. Timmins ◽  
J. Witz
Keyword(s):  
Mosaic Virus ◽  
Plant Virus ◽  
Cauliflower Mosaic Virus

scholarly journals Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Valentina Turri ◽  
Olga S. Latinovic ◽  
Massimiliano Bonafè ◽  
Ngeh Toyang ◽  
Maria Parigi ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Plant Virus ◽  
Sequence Similarity ◽  
Genetic Material ◽  
Glioma Cells ◽  
Human Cells ◽  
Cauliflower Mosaic Virus ◽  
Virus Protein ◽  
Human Beings

Recent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells.

scholarly journals Water deficit changes the relationships between epidemiological traits of Cauliflower mosaic virus across diverse Arabidopsis thaliana accessions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sandy E. Bergès ◽  
Denis Vile ◽  
Michel Yvon ◽  
Diane Masclef ◽  
Myriam Dauzat ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mosaic Virus ◽  
Water Deficit ◽  
Abiotic Stresses ◽  
Plant Virus ◽  
Transmission Rate ◽  
Horizontal Transmission ◽  
Cauliflower Mosaic Virus ◽  
Significant Relationships ◽  
Response To Water

AbstractChanges in plant abiotic environments may alter plant virus epidemiological traits, but how such changes actually affect their quantitative relationships is poorly understood. Here, we investigated the effects of water deficit on Cauliflower mosaic virus (CaMV) traits (virulence, accumulation, and vectored-transmission rate) in 24 natural Arabidopsis thaliana accessions grown under strictly controlled environmental conditions. CaMV virulence increased significantly in response to water deficit during vegetative growth in all A. thaliana accessions, while viral transmission by aphids and within-host accumulation were significantly altered in only a few. Under well-watered conditions, CaMV accumulation was correlated positively with CaMV transmission by aphids, while under water deficit, this relationship was reversed. Hence, under water deficit, high CaMV accumulation did not predispose to increased horizontal transmission. No other significant relationship between viral traits could be detected. Across accessions, significant relationships between climate at collection sites and viral traits were detected but require further investigation. Interactions between epidemiological traits and their alteration under abiotic stresses must be accounted for when modelling plant virus epidemiology under scenarios of climate change.

scholarly journals Identification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors

2018 ◽  
Vol 92 (14) ◽  
Author(s):  
Craig G. Webster ◽  
Elodie Pichon ◽  
Manuella van Munster ◽  
Baptiste Monsion ◽  
Maëlle Deshoux ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Myzus Persicae ◽  
Plant Virus ◽  
Plant Viruses ◽  
Cauliflower Mosaic Virus ◽  
Insect Vectors ◽  
Virus Receptor ◽  
Content Type ◽  
Cuticular Proteins

ABSTRACTPlant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a noncirculative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphidAcyrthosiphon pisumand the green peach aphidMyzus persicae, in the acrostyle, an organ earlier shown to harbor receptors of a noncirculative virus. A peptide motif present at the C termini of both Stylin-01 and Stylin-02 is readily accessible all over the surface of the acrostyle. Competition forin vitrobinding to the acrostyle was observed between an antibody targeting this peptide and the helper component protein P2 ofCauliflower mosaic virus. Furthermore, silencing thestylin-01but notstylin-02gene through RNA interference decreased the efficiency ofCauliflower mosaic virustransmission byMyzus persicae. These results identify the first cuticular proteins ever reported within arthropod mouthparts and distinguish Stylin-01 as the best candidate receptor for the aphid transmission of noncirculative plant viruses.IMPORTANCEMost noncirculative plant viruses transmitted by insect vectors bind to their mouthparts. They are acquired and inoculated within seconds when insects hop from plant to plant. The receptors involved remain totally elusive due to a long-standing technical bottleneck in working with insect cuticle. Here we characterize the role of the two first cuticular proteins ever identified in arthropod mouthparts. A domain of these proteins is directly accessible at the surface of the cuticle of the acrostyle, an organ at the tip of aphid stylets. The acrostyle has been shown to bind a plant virus, and we consistently demonstrated that one of the identified proteins is involved in viral transmission. Our findings provide an approach to identify proteins in insect mouthparts and point at an unprecedented gene candidate for a plant virus receptor.

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