Stage of networking is quintessential task in which security comes into play. Securing these networks which contains confidential digital data that needs to secured will be the agenda of cryptography. Many cryptographic algorithms increment their strengths over parameters like key size, increasing the rounds of iteration and finally using confusion box as S-box as it has best robustness. So, this paper mainly focusses over securing digital data with the help of S-box function over Data Encryption Standard (DES) algorithm. For this, a plain text and key will be given to this DES as it extracts 8x8(64) bit characters from the key and converting them into its corresponding ASCII value and are concatenating to form an 8 value by taking mod16. These will give to 8 S-box in order to generate its corresponding output to make even more secure and also shows dynamic DES gives much result than other crypto methods. The evaluation of this integrated s-box and DES shows much fruitful results over factors like non-linearity, Avalanche criterion, Balance, Robustness to linear cryptanalysis, Robustness to differential cryptanalysis.