Nitrogen and Growth Regulator Effects on Shoot and Root Growth of Soybean in vitro

Author(s):  
Ralph L. Mott ◽  
John M. Cordts ◽  
Ann M. Larson
Author(s):  
Luciana Cardoso Nogueira Londe ◽  
Wagner A. Vendrame ◽  
Alexandre Bosco de Oliveira ◽  
Maurício Mendes Cardoso

Micropropagation techniques represent one of the technologies, which allows the large-scale production of banana and the culture medium composition is one of the major factors affecting in vitro propagation of plants. The objective of this study was to evaluate the efficiency of a new micronutrient delivery system based on ionic Cu and Zn for inducing in vitro organogenesis in two banana cultivars in vitro cultures, Grand Naine and Pysang Ceylon. The first experiment evaluated different concentrations of BAM-FX® (0.16, 0.32, 0.64, 1.28, and 2.56 µl ml-1) added to the MS culture medium. The concentration of 0.16 µl ml-1 BAM-FX® provided the best results for in vitro shoot and root growth and development. Therefore, a second experiment was performed to evaluate the potential of combining BAM-FX® with a reduced concentration of MS medium (¼, ½, and ¾ strength), or the use of BAM-FX® alone without MS medium. Results indicate that MS at ¾ strength combined with 0.16 µl ml-1 BAM-FX® provided proper in vitro shoot and root growth and development. The use of BAM-FX® in vitro requires additional studies to verify the feasibility of this product for efficient micropropagation of banana, as well as for other species.


Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 611
Author(s):  
Abdessamad Fakhech ◽  
Martin Jemo ◽  
Najat Manaut ◽  
Lahcen Ouahmane ◽  
Mohamed Hafidi

The impact of salt stress on the growth and phosphorus utilization efficiency (PUE) of two leguminous species: Retama monosperma and Acacia gummifera was studied. The effectiveness of arbuscular mycorrhizal fungi (AMF) to mitigate salt stress was furthermore assessed. Growth, N and P tissue concentrations, mycorrhizal root colonization frequency and intensity, and P utilization efficiency (PUE) in the absence or presence of AMF were evaluated under no salt (0 mM L−1) and three salt (NaCl) concentrations of (25, 50 and 100 mM L−1) using a natural sterilized soil. A significant difference in mycorrhizal colonization intensity, root-to-shoot ratio, P uptake, PUE, and N uptake was observed between the legume species. Salt stress inhibited the shoot and root growth, and reduced P and N uptake by the legume species. Mycorrhizal inoculation aided to mitigate the effects of salt stress with an average increase of shoot and root growth responses by 35% and 32% in the inoculated than in the non-inoculated A. gummifera treatments. The average shoot and root growth responses were 37% and 45% higher in the inoculated compared to the non-inoculated treatments of R. monosperma. Average mycorrhizal shoot and root P uptake responses were 66% and 68% under A. gummifera, and 40% and 95% under R. monosperma, respectively. Mycorrhizal inoculated treatments consistently maintained lower PUE in the roots. The results provide insights for further investigations on the AMF conferred mechanisms to salt stress tolerance response by A. gummifera and R. monosperma, to enable the development of effective technologies for sustainable afforestation and reforestation programs in the Atlantic coast of Morocco.


2000 ◽  
Vol 51 (345) ◽  
pp. 731-737
Author(s):  
José A. Fernández ◽  
Julio Escribano ◽  
Abel Piqueras ◽  
Joaquín Medina

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   


2004 ◽  
Vol 16 (6) ◽  
pp. 1365-1377 ◽  
Author(s):  
Chika Nishimura ◽  
Yoshi Ohashi ◽  
Shusei Sato ◽  
Tomohiko Kato ◽  
Satoshi Tabata ◽  
...  

2013 ◽  
Vol 150 ◽  
pp. 317-325 ◽  
Author(s):  
Aye Aye Thwe ◽  
Gilles Vercambre ◽  
Hélène Gautier ◽  
Loïc Pagès ◽  
Christophe Jourdan ◽  
...  

2018 ◽  
Vol 223 ◽  
pp. 65-71 ◽  
Author(s):  
Aloka Kumari ◽  
Ponnusamy Baskaran ◽  
Lenka Plačková ◽  
Hana Omámiková ◽  
Jaroslav Nisler ◽  
...  

2003 ◽  
Vol 3 (6) ◽  
pp. 553-560 ◽  
Author(s):  
M.Z. Karim . ◽  
M.N. Amin . ◽  
M.A.K. Azad . ◽  
F. Begum . ◽  
M.M. Rahman . ◽  
...  

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