Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies

2017 ◽  
pp. 117-136 ◽  
Author(s):  
Lucas Moyet ◽  
Daniel Salvi ◽  
Martino Tomizioli ◽  
Daphné Seigneurin-Berny ◽  
Norbert Rolland
Keyword(s):  
Stroma Lamellae

scholarly journals FINE STRUCTURAL CHANGES IN PROPLASTIDS DURING PHOTODESTRUCTION OF PIGMENTS

1964 ◽  
Vol 22 (2) ◽  
pp. 443-451 ◽  
Author(s):  
Shimon Klein ◽  
Lawrence Bogorad
Keyword(s):  
Fine Structure ◽  
Levulinic Acid ◽  
Structural Changes ◽  
Prolamellar Body ◽  
Membrane Structures ◽  
Lamellar Structures ◽  
Amino Levulinic Acid ◽  
Stroma Lamellae ◽  
Green Pigments ◽  
Bean Leaves

Etiolated bean leaves supplied δ-amino-levulinic acid in the dark synthesize large amounts of protochlorophyllide which is not converted to chlorophyllide upon illumination of the leaves. The fine structure of the proplastids is not affected by the treatment. When leaves containing "inactive" protochlorophyllide are exposed to light of 700 ft-c for 3 hours, they lose practically all their green pigments. During this period large stacks of closed membrane structures are built up in the region of the prolamellar body. These lamellar structures remain even when no or only traces of pigment are left in the leaves. In untreated control leaves the pigment content remained constant during similar illumination and the structural changes in the plastids consisted of a rearrangement of the vesicles from the prolamellar bodies into strands dispersed through the stroma; lamellae and grana formation occurred later.

scholarly journals Biosynthesis of Grana and Stroma Lamellae in Spinach

1974 ◽  
Vol 54 (3) ◽  
pp. 386-391 ◽  
Author(s):  
Fernando Henriques ◽  
Roderic Park
Keyword(s):  
Stroma Lamellae

scholarly journals Lateral distribution and diffusion of plastocyanin in chloroplast thylakoids.

1989 ◽  
Vol 108 (4) ◽  
pp. 1397-1405 ◽  
Author(s):  
W Haehnel ◽  
R Ratajczak ◽  
H Robenek
Keyword(s):  
Photosystem I ◽  
Laser Flash ◽  
Lateral Distribution ◽  
High Time Resolution ◽  
High Concentration ◽  
Long Distance ◽  
Kinetic Measurements ◽  
Stroma Lamellae ◽  
Negative Surface ◽  
Negative Surface Charge

The lateral distribution of plastocyanin in the thylakoid lumen of spinach and pea chloroplasts was studied by combining immunocytochemical localization and kinetic measurements of P700+ reduction at high time resolution. In dark-adapted chloroplasts, the concentration of plastocyanin in the photosystem I containing stroma membranes exceeds that in photosystem II containing grana membranes by a factor of about two. Under these conditions, the reduction of P700+ with a halftime of 12 microseconds after a laser flash of saturating intensity indicates that to greater than 95% of total photosystem I a plastocyanin molecule is bound. An analysis of the labeling densities, the length of the different lumenal regions, and the total amounts of plastocyanin and P700 shows that most of the remaining presumable mobile plastocyanin is found in the granal lumen. This distribution of plastocyanin is consistent with a more negative surface charge density in the stromal than in the granal lumen. During illumination the concentration of plastocyanin in grana increases at the expense of that in stroma lamellae, indicating a light-driven diffusion from stroma to grana regions. Our observations provide evidence that a high concentration of plastocyanin in grana in the light favors the lateral electron transport from cytochrome b6/f complexes in appressed grana across the long distance to photosystem I in nonappressed stroma membranes.

scholarly journals Fundamental helical geometry consolidates the plant photosynthetic membrane

2019 ◽  
Vol 116 (44) ◽  
pp. 22366-22375 ◽  
Author(s):  
Yuval Bussi ◽  
Eyal Shimoni ◽  
Allon Weiner ◽  
Ruti Kapon ◽  
Dana Charuvi ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Photosynthetic Membrane ◽  
Lamellar System ◽  
Left Handed ◽  
Dense Nuclear Matter ◽  
Theoretical Predictions ◽  
Stroma Lamellae ◽  
Helical Geometry ◽  
The Right ◽  
Efficient Packing

Plant photosynthetic (thylakoid) membranes are organized into complex networks that are differentiated into 2 distinct morphological and functional domains called grana and stroma lamellae. How the 2 domains join to form a continuous lamellar system has been the subject of numerous studies since the mid-1950s. Using different electron tomography techniques, we found that the grana and stroma lamellae are connected by an array of pitch-balanced right- and left-handed helical membrane surfaces of different radii and pitch. Consistent with theoretical predictions, this arrangement is shown to minimize the surface and bending energies of the membranes. Related configurations were proposed to be present in the rough endoplasmic reticulum and in dense nuclear matter phases theorized to exist in neutron star crusts, where the right- and left-handed helical elements differ only in their handedness. Pitch-balanced helical elements of alternating handedness may thus constitute a fundamental geometry for the efficient packing of connected layers or sheets.

scholarly journals Carotenoid Composition of Spinach Chloroplast Grana and Stroma Lamellae

1973 ◽  
Vol 51 (3) ◽  
pp. 584-585 ◽  
Author(s):  
T. Trosper ◽  
C. Freeman Allen
Keyword(s):  
Spinach Chloroplast ◽  
Carotenoid Composition ◽  
Stroma Lamellae
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