Investigation of Peptide Biomarker Stability in Plasma Samples Using Time-Course MS Analysis

2011 ◽  
pp. 161-175 ◽  
Author(s):  
Jizu Yi ◽  
Zhaoxia Liu ◽  
Craig A. Gelfand ◽  
David Craft
Keyword(s):  
Time Course ◽  
Plasma Samples ◽  
Ms Analysis

scholarly journals Proteomic identification of biomarkers in maternal plasma that predict the outcome of rescue cerclage for cervical insufficiency

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250031
Author(s):  
Kisoon Dan ◽  
Ji Eun Lee ◽  
Dohyun Han ◽  
Sun Min Kim ◽  
Subeen Hong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Multiple Reaction Monitoring ◽  
Area Under The Curve ◽  
Maternal Plasma ◽  
Tandem Mass ◽  
Cervical Insufficiency ◽  
Plasma Samples ◽  
Biomarker Panel ◽  
Ms Analysis

Objective We sought to identify plasma protein biomarkers that are predictive of the outcome of rescue cerclage in patients with cervical insufficiency. Methods This retrospective cohort study included 39 singleton pregnant women undergoing rescue cerclage for cervical insufficiency (17–25 weeks) who gave plasma samples. Three sets of pooled plasma samples from controls (cerclage success, n = 10) and cases (cerclage failure, n = 10, defined as spontaneous preterm delivery at <33 weeks) were labeled with 6-plex tandem mass tag (TMT) reagents and analyzed by liquid chromatography-tandem mass spectrometry. Differentially expressed proteins between the two groups were selected from the TMT-based quantitative analysis. Multiple reaction monitoring-mass spectrometry (MRM-MS) analysis was further used to verify the candidate proteins of interest in patients with cervical insufficiency in the final cohort (n = 39). Results From MRM-MS analysis of the 40 proteins showing statistically significant changes (P < 0.05) from the TMT-based quantitative analysis, plasma IGFBP-2, PSG4, and PGLYRP2 levels were found to be significantly increased, whereas plasma MET and LXN levels were significantly decreased in women with cerclage failure. Of these, IGFBP-2, PSG4, and LXN levels in plasma were independent of cervical dilatation. A multiple-biomarker panel was developed for the prediction of cerclage failure, using a stepwise regression procedure, which included the plasma IGFBP-2, PSG4, and LXN (area under the curve [AUC] = 0.916). The AUC for this multiple-biomarker panel was significantly greater than the AUC for any single biomarker included in the multi-biomarker model. Conclusions Proteomic analysis identified useful and independent plasma biomarkers (IGFBP-2, PSG4, and LXN; verified by MRM) that predict poor pregnancy outcome following rescue cerclage. Their combined analysis in a multi-biomarker panel significantly improved predictability.

Persistant Inhibition of Thrombin Generation After Intravenous Administration of Enoxaparin in Primates.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2095-2095
Author(s):  
Evangelos Litinas ◽  
Angel Gray ◽  
Nasir Sadeghi ◽  
Josephine Cunanan ◽  
Debra Hoppensteadt ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Time Course ◽  
Conflicts Of Interest ◽  
Clinical Effects ◽  
Plasma Samples ◽  
Thrombin Inhibition ◽  
Biologic Effects ◽  
Antithrombotic Effects ◽  
Sustained Inhibition ◽  
Inhibition Of Thrombin

Abstract Abstract 2095 Poster Board II-72 The biologic half life (T12) of low molecular weight heparin (LMWH) is usually measured in terms of the circulating anti-Xa levels. Enoxaparin represents an unique LMWH whose biologic T12 is relatively longer than most LMWHs. Moreover, it is known that the antithrombotic effects of this agent last longer in comparison to the measurable circulating anti-Xa levels. Therefore besides the anti-Xa activity, additional non-measurable biologic effects are contributory to the clinical effects of this agent. Plasma based thrombin generation assays have recently become available to assess the effects of LMWHs such as enoxaparin. In these assays blood plasma samples are activated using different activators and the generated thrombin inhibition is measured. To measure the time course of thrombin generation inhibitory activity after an IV bolus dose of 0.5 mg/kg of enoxaparin into groups of primates (n=6-8), a commercially available thrombin generation method was employed (Technoclone, Vienna, Austria/DiaPharma, West Chester,OH). Blood samples were drawn from each of the primates injected at varying time points for up to 28 hours. A thromboplastin/phospholipids based reagent was used to generate thrombin and the results were recorded in terms of nm of thrombin formed. The baseline values ranged from 500-900 nm (710±60 nm), although a complete inhibition of thrombin generation was noted at 1 hour (24±8 nm), a slow and gradual reduction in the thrombin generation inhibition was noted with a T12 of 9 hours. Even at 28 hours after the administration of enoxaparin, sustained inhibition of thrombin generation was noted (30-50%). Interestingly, the circulating anti-Xa and anti-IIa activity gradually diminished to an almost non-detectable level at 6 hours. These studies suggest that enoxaparin produces antithrombotic actions by multiple mechanisms. Furthermore thrombin generation methods in plasma samples may provide a more sensitive assay for the monitoring of the effect of LMWH. Disclosures: No relevant conflicts of interest to declare.

An Innovative Approach to the Preparation of Plasma Samples for UHPLC–MS Analysis

2019 ◽  
Vol 67 (23) ◽  
pp. 6665-6671 ◽  
Author(s):  
Michael Kaiser ◽  
Bartosch Lacheta ◽  
Maike Passon ◽  
Andreas Schieber
Keyword(s):  
Innovative Approach ◽  
Plasma Samples ◽  
Ms Analysis

scholarly journals An alternative extrinsic pathway of human blood coagulation

Blood ◽  
1982 ◽  
Vol 60 (6) ◽  
pp. 1353-1358 ◽  
Author(s):  
RA Marlar ◽  
AJ Kleiss ◽  
JH Griffin
Keyword(s):  
Time Course ◽  
Factor Ix ◽  
Rabbit Brain ◽  
Plasma Samples ◽  
Factor X ◽  
Extrinsic Pathway ◽  
Clinical Observations ◽  
Activation Rate ◽  
Factor X Activation ◽  
Human Blood Coagulation

Abstract To study the interrelationships of the major human coagulation pathways, factor X activation in normal and various deficient human plasmas was evaluated when clotting was triggered by dilute rabbit or human thromboplastin. Various dilutions of thromboplastin were added to plasma samples containing 3H-labeled factor X, and the time course of factor X activation was determined. At a 1/250 dilution of rabbit brain thromboplastin the rate of factor X activation in factor VIII or factor IX deficient plasma was only 10% of the activation rate seen for normal or factor XI deficient plasma. Reconstitution of the deficient plasmas with factors VIII or IX, respectively, restored normal factor X activation. Similar results were obtained when various dilutions of human thromboplastin replaced the rabbit thromboplastin. From these experiments, it is inferred that normal activation of factor X in plasma due to dilute thromboplastin requires factors VII, IX and VIII. An alternative extrinsic pathway that involves factors VII, IX, and VIII may be a major physiologic extrinsic pathway, and this pathway may help to explain the clinical observations of bleeding diatheses in patients deficient in factors IX or VIII.

On-Line SPE on Restricted Access Adsorbent for HPLC-MS/MS Analysis of Felodipine in Plasma Samples

2010 ◽  
Vol 43 (7-8) ◽  
pp. 1330-1343 ◽  
Author(s):  
Florin Albu ◽  
Iuliana Sora ◽  
Florentin Tache ◽  
Victor David ◽  
Andrei Medvedovici
Keyword(s):  
Plasma Samples ◽  
Restricted Access ◽  
Ms Analysis ◽  
On Line

Stress-induced disruption of parturition in the rat may be mediated by endogenous opioids

1987 ◽  
Vol 114 (2) ◽  
pp. 247-252 ◽  
Author(s):  
G. Leng ◽  
S. Mansfield ◽  
R. J. Bicknell ◽  
D. Brown ◽  
C. Chapman ◽  
...  
Keyword(s):  
Time Course ◽  
Endogenous Opioids ◽  
Opioid Antagonist ◽  
Plasma Samples ◽  
Conscious Rats ◽  
Glass Chamber ◽  
Oxytocin Release ◽  
Human Parturition

ABSTRACT Plasma samples were obtained before and during parturition from conscious rats implanted chronically with a jugular cannula. Rats were either allowed to remain in their nesting cage throughout parturition, or were moved immediately following the birth of the second or third pup into an empty glass chamber. The time-course of parturition was prolonged for mother rats which were moved in mid-parturition to this unfamiliar environment. However, in rats given an i.v. injection of the opioid antagonist naloxone at the time of transfer, parturition followed a normal time-course, and plasma oxytocin levels were significantly higher than in animals injected with saline. Thus our hypothesis is that stress activates opioid pathways which delay parturition by inhibiting oxytocin release. Opioid-mediated mechanisms may similarly be responsible for some problems in human parturition. J. Endocr. (1987) 114, 247–252

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