Methods for In Vivo and In Vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

2012 ◽  
pp. 209-225 ◽  
Author(s):  
Milan K. Patel ◽  
Glennice N. Ryan ◽  
Anna M. Cerny ◽  
Evelyn A. Kurt-Jones
Keyword(s):  
Helicobacter Pylori ◽  
Immune Responses ◽  
Innate Immune ◽  
Helicobacter Pylori Infection ◽  
Innate Immune Responses ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo

2020 ◽  
Vol 11 ◽  
Author(s):  
Gaël Auray ◽  
Stephanie C. Talker ◽  
Irene Keller ◽  
Sylvie Python ◽  
Markus Gerber ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
High Resolution ◽  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Dendritic Cell Subsets

194 A Non-Gluten Component of Wheat is a Strong Stimulator of Innate Immune Responses In Vitro and In Vivo and Acts via Toll Like Receptor 4

2010 ◽  
Vol 138 (5) ◽  
pp. S-36
Author(s):  
Yvonne Junker ◽  
Donatella Barisani ◽  
Daniel A. Leffler ◽  
Towia Libermann ◽  
Simon T. Dillon ◽  
...  
Keyword(s):  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

in vivo and in vitro analysis of the functional role of Epstein-Barr virus-induced gene 3 (EBI3) for regulation of Th2 mediated immune responses

2003 ◽  
Vol 124 (4) ◽  
pp. A335
Author(s):  
Stefan J. Wirtz ◽  
Christoph Becker ◽  
Edward E.S. Nieuwenhuis ◽  
Mark Birkenbach ◽  
Richard S. Blumberg ◽  
...  
Keyword(s):  
Immune Responses ◽  
Functional Role ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Polysaccharides derived from Yamoa™ (Funtumia elastica) prime γδ T cells in vitro and enhance innate immune responses in vivo

2009 ◽  
Vol 9 (11) ◽  
pp. 1313-1322 ◽  
Author(s):  
Jill C. Graff ◽  
Emily M. Kimmel ◽  
Brett Freedman ◽  
Igor A. Schepetkin ◽  
Jeff Holderness ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Γδ T Cells ◽  
Innate Immune ◽  
Innate Immune Responses

scholarly journals IFNα and IFNγ Impede Marek’s Disease Progression

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1103 ◽  
Author(s):  
Luca D. Bertzbach ◽  
Olof Harlin ◽  
Sonja Härtle ◽  
Frank Fehler ◽  
Tereza Vychodil ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Onset ◽  
Antiviral Effect ◽  
Lymphoproliferative Disease ◽  
Innate Immune ◽  
Marek's Disease ◽  
Marek’S Disease ◽  
Innate Immune Responses

Marek’s disease virus (MDV) is an alphaherpesvirus that causes Marek’s disease, a malignant lymphoproliferative disease of domestic chickens. While MDV vaccines protect animals from clinical disease, they do not provide sterilizing immunity and allow field strains to circulate and evolve in vaccinated flocks. Therefore, there is a need for improved vaccines and for a better understanding of innate and adaptive immune responses against MDV infections. Interferons (IFNs) play important roles in the innate immune defenses against viruses and induce upregulation of a cellular antiviral state. In this report, we quantified the potent antiviral effect of IFNα and IFNγ against MDV infections in vitro. Moreover, we demonstrate that both cytokines can delay Marek’s disease onset and progression in vivo. Additionally, blocking of endogenous IFNα using a specific monoclonal antibody, in turn, accelerated disease. In summary, our data reveal the effects of IFNα and IFNγ on MDV infection and improve our understanding of innate immune responses against this oncogenic virus.

scholarly journals Induction of Innate Immune Responses by SIV In Vivo and In Vitro: Differential Expression and Function of RIG-I and MDA5

2011 ◽  
Vol 204 (7) ◽  
pp. 1104-1114 ◽  
Author(s):  
Juliene G. Co ◽  
Kenneth W. Witwer ◽  
Lucio Gama ◽  
M. Christine Zink ◽  
Janice E. Clements
Keyword(s):  
Immune Responses ◽  
Differential Expression ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
And Function

scholarly journals Pneumolysin, PspA, and PspC Contribute to Pneumococcal Evasion of Early Innate Immune Responses during Bacteremia in Mice

2007 ◽  
Vol 75 (4) ◽  
pp. 2067-2070 ◽  
Author(s):  
Lisa R. Quin ◽  
Quincy C. Moore ◽  
Larry S. McDaniel
Keyword(s):  
Immune Responses ◽  
Virulence Factors ◽  
Innate Immune ◽  
Blood Clearance ◽  
Innate Immune Responses

ABSTRACTThe pneumococcal virulence factors include capsule, PspA, PspC, and Ply. Cytometric analysis demonstrated that the greatest levels of C3 deposition were on a ΔplyPspA−PspC−mutant. Also, Ply, PspA, and PspC expression resulted in C3 degradation in vitro and in vivo. Finally, blood clearance assays demonstrated that there was enhanced clearance of ΔplyPspA−PspC−pneumococci compared to the clearance of nonencapsulated pneumococci.

scholarly journals Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS

2019 ◽  
Vol 218 (2) ◽  
pp. 700-721 ◽  
Author(s):  
Fang Niu ◽  
Ke Liao ◽  
Guoku Hu ◽  
Susmita Sil ◽  
Shannon Callen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Active Components ◽  
Barrier Integrity ◽  
Enhanced Secretion ◽  
First Time ◽  
The Brain

Cocaine is known to facilitate the transmigration of inflammatory leukocytes into the brain, an important mechanism underlying neuroinflammation. Pericytes are well-recognized as important constituents of the blood–brain barrier (BBB), playing a key role in maintaining barrier integrity. In the present study, we demonstrate for the first time that exposure of human brain vascular pericytes to cocaine results in enhanced secretion of CXCL10, leading, in turn, to increased monocyte transmigration across the BBB both in vitro and in vivo. This process involved translocation of σ-1 receptor (σ-1R) and interaction of σ-1R with c-Src kinase, leading to activation of the Src–PDGFR-β–NF-κB pathway. These findings imply a novel role for pericytes as a source of CXCL10 in the pericyte–monocyte cross talk in cocaine-mediated neuroinflammation, underpinning their role as active components of the innate immune responses.

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