Worldwide Distribution and Identification of Grapevine Yellows Diseases

2017 ◽  
pp. 17-46 ◽  
Author(s):  
Fiona Constable ◽  
Assunta Bertaccini
Keyword(s):  
Grapevine Yellows ◽  
Worldwide Distribution ◽  
Yellows Diseases

Detection of Phytoplasmas Associated to Grapevine Yellows Diseases in Research and Diagnostics

2017 ◽  
pp. 69-95
Author(s):  
Nataša Mehle ◽  
Maja Ravnikar ◽  
Magda Tušek Žnidarič ◽  
Amal Aryan ◽  
Günter Brader ◽  
...  
Keyword(s):  
Grapevine Yellows ◽  
Yellows Diseases

Grapevine Yellows Diseases and Their Phytoplasma Agents

2017 ◽  
Author(s):  
Marina Dermastia ◽  
Assunta Bertaccini ◽  
Fiona Constable ◽  
Nataša Mehle
Keyword(s):  
Grapevine Yellows ◽  
Yellows Diseases

scholarly journals Yellow Disease of Grapevines: Impact, Pathogen Molecular Detection and Identification, Epidemiology, and Potential for Control

1994 ◽  
Author(s):  
Robert E. Davis ◽  
Edna Tanne ◽  
James P. Prince ◽  
Meir Klein
Keyword(s):  
United States ◽  
The United States ◽  
Effective Control ◽  
Molecular Diagnostic ◽  
Rrna Gene ◽  
Grapevine Yellows ◽  
Weed Host ◽  
Detection And Identification ◽  
The Usa ◽  
Yellows Diseases

Grapevine yellows diseases characterized by similar symptoms have been reported in several countries including Israel, the United States, France, Italy, Spain, Germany and Australia. These diseases are among the most serious known in grapevine, but precise knowledge of the pathogens' identities and modes of their spread is needed to devise effective control stratgegies. The overall goals of this project were to develop improved molecular diagnostic procedures for detection and identification of the presumed mycoplasmalike organism (MLO) pathogens, now termed phytoplasmas, and to apply these procedures to investigate impact and spread and potential for controlling grapevine yellows diseases. In the course of this research project, increased incidence of grapevine yellows was found in Israel and the United States; the major grapevine yellows phytoplasma in Israel was identified and tis 16S rRNA gene characterized; leafhopper vectors of this grapevine yellows phytoplasma in Israel were identified; a second phytoplasma was discovered in diseased grapevines in Israel; the grapevine yellows disease in the U.S. was found to be distinct from that in Israel; grapevine yellows in Virginia, USA, was found to be caused by two different phytoplasmas; both phytoplasmas in Virginia grapevines were molecularly characterized and classified; commercial grapevines in Europe were discovered to host a phytoplasma associated with aster yellow disease in the USA, but this phytoplasma has not been found in grapevine in the USA; the Australian grapevine yellows phytoplasma was found to be distinct from the grapevine phytoplasmas in Israel, the United States and Europe and was described and named "Candidatus phytoplasma australiense", and weed host plants acting as potential reservoirs of the grapevine phytoplasmas were discovered. These and other findings from the project should aid in the design and development of strategies for managing the grapevine yellows disease problem.

A rapid method for the direct identification of paramyxovirus in canine CSF by ultracentrifugation and negative staining as a rule out for distemper

1990 ◽  
Vol 48 (3) ◽  
pp. 594-595
Author(s):  
W.L. Steffens ◽  
M.B. Ard ◽  
C.E. Greene ◽  
A. Jaggy
Keyword(s):  
Subacute Sclerosing Panencephalitis ◽  
Clinical Signs ◽  
Viral Disease ◽  
Etiologic Agent ◽  
Mucosal Inflammation ◽  
Worldwide Distribution ◽  
Negative Stain ◽  
Viral Particles ◽  
Systemic Manifestations ◽  
Rule Out

Canine distemper is a multisystemic contagious viral disease having a worldwide distribution, a high mortality rate, and significant central neurologic system (CNS) complications. In its systemic manifestations, it is often presumptively diagnosed on the basis of clinical signs and history. Few definitive antemortem diagnostic tests exist, and most are limited to the detection of viral antigen by immunofluorescence techniques on tissues or cytologic specimens or high immunoglobulin levels in CSF (cerebrospinal fluid). Diagnosis of CNS distemper is often unreliable due to the relatively low cell count in CSF (<50 cells/μl) and the binding of blocking immunoglobulins in CSF to cell surfaces. A more reliable and definitive test might be possible utilizing direct morphologic detection of the etiologic agent. Distemper is the canine equivalent of human measles, in that both involve a closely related member of the Paramyxoviridae, both produce mucosal inflammation, and may produce CNS complications. In humans, diagnosis of measles-induced subacute sclerosing panencephalitis is through negative stain identification of whole or incomplete viral particles in patient CSF.

Worldwide Distribution of Sulfadoxine-Pyrimethamine Resistant Malaria Parasites

2020 ◽  
Author(s):  
Rini Chaturvedi ◽  
Jyoti Chhibber-Goel ◽  
Ishika Verma ◽  
Sreehari Gopinathan ◽  
Suhel Parvez ◽  
...  
Keyword(s):  
Malaria Parasites ◽  
Worldwide Distribution

scholarly journals Secondary Metabolites from Artemisia Genus as Biopesticides and Innovative Nano-Based Application Strategies

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3061
Author(s):  
Bianca Ivănescu ◽  
Ana Flavia Burlec ◽  
Florina Crivoi ◽  
Crăița Roșu ◽  
Andreia Corciovă
Keyword(s):  
Secondary Metabolites ◽  
Negative Impact ◽  
Crop Protection ◽  
Duration Of Action ◽  
Worldwide Distribution ◽  
Artemisia Species ◽  
Extended Duration ◽  
Reduced Toxicity ◽  
Available Information ◽  
Potential Use

The Artemisia genus includes a large number of species with worldwide distribution and diverse chemical composition. The secondary metabolites of Artemisia species have numerous applications in the health, cosmetics, and food sectors. Moreover, many compounds of this genus are known for their antimicrobial, insecticidal, parasiticidal, and phytotoxic properties, which recommend them as possible biological control agents against plant pests. This paper aims to evaluate the latest available information related to the pesticidal properties of Artemisia compounds and extracts and their potential use in crop protection. Another aspect discussed in this review is the use of nanotechnology as a valuable trend for obtaining pesticides. Nanoparticles, nanoemulsions, and nanocapsules represent a more efficient method of biopesticide delivery with increased stability and potency, reduced toxicity, and extended duration of action. Given the negative impact of synthetic pesticides on human health and on the environment, Artemisia-derived biopesticides and their nanoformulations emerge as promising ecofriendly alternatives to pest management.

scholarly journals Molecular Identification of New Cases of Human Dirofilariosis (Dirofilaria repens) in Italy

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 251
Author(s):  
Simona Gabrielli ◽  
Valentina Mangano ◽  
Federica Furzi ◽  
Alessandra Oliva ◽  
Serena Vita ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Central Italy ◽  
Research Work ◽  
Pulmonary Nodules ◽  
Elisa Test ◽  
Future Research ◽  
Worldwide Distribution ◽  
Human Dirofilariosis ◽  
Vector Borne ◽  
Etiological Agents

(1) Dirofilariosis is a vector-borne parasitic disease mainly in domestic and wild carnivores caused by Dirofilaria (Noctiella) repens, which is endemic in many countries of the Old World, and D. immitis, which has a worldwide distribution. In recent years, an increase in the number of human cases has been reported, suggesting that dirofilariosis is an emergent zoonosis. Here, we describe further cases (N = 8), observed in Central Italy during the years 2018–2019. (2) Molecular diagnosis was performed on: (i) live worms extracted from ocular conjunctiva, cheek, and calf muscle; (ii) histological sections of surgically removed nodules from parenchymal lung, coccyx, and breast. (3) Sequence analysis (650-bp) of the mitochondrial cytochrome oxidase subunit I gene (mtDNA cox1) showed a match of 100% with the sequences of D. repens previously deposited in GenBank. ELISA test to detect IgG against filarial antigens was performed on four patients’ sera and resulted positive in two patients who showed ocular and subcutaneous dirofilariosis, respectively. Microfilariae have been never detected in the peripheral blood of the patients. (4) The occurrence of N = 8 new cases of human D. repens-infections observed in a two-year period suggests an increased circulation of the parasite in Italy. Therefore, dirofilariosis should be included in differential diagnosis in patients presenting subcutaneous and/or pulmonary nodules. Molecular diagnosis of the etiological agents is fundamental. Specific serological diagnosis needs to be improved in future research work.

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