Common antigenic determinant on ductular cells of normal pancreas, on mucosal cells of the gastrointestinal tract and on CEA

1990 ◽  
pp. 628-629
Author(s):  
W Dippold ◽  
A Steinborn ◽  
R Klingel ◽  
K-H Meyer
Keyword(s):  
Gastrointestinal Tract ◽  
Antigenic Determinant ◽  
Normal Pancreas ◽  
Mucosal Cells

scholarly journals Distribution of glucagon-like peptide I in canine and feline pancreas and gastrointestinal tract.

1986 ◽  
Vol 34 (9) ◽  
pp. 1117-1121 ◽  
Author(s):  
C R Vaillant ◽  
P K Lund
Keyword(s):  
Gastrointestinal Tract ◽  
Endocrine Cells ◽  
Northern Blot Analysis ◽  
Islet Cells ◽  
Cleavage Product ◽  
Antigenic Determinants ◽  
Normal Pancreas ◽  
Fetal Rat ◽  
Islet Tissue ◽  
Glucagon Like Peptide

Recently, a putative hormone, glucagon-like peptide I (GLP I), has been identified in the predicted sequences of the precursors to pancreatic glucagon in human, rat, hamster, and ox. The distribution of GLP I immunoreactivity in canine and feline pancreas and gastrointestinal tract was examined immunohistochemically and was compared with that of two other antigenic determinants of pancreatic pro-glucagon, i.e., glucagon and the NH2 terminus of glicentin. All three determinants occurred in the same population of islet cells in normal pancreas and in pancreas consisting predominantly of islet tissue from dogs with canine pancreatic acinar atrophy. Northern blot analysis of mRNA from the latter tissue, using a rat pre-pro-glucagon complementary DNA probe, revealed a single mRNA species similar in size to the pre-pro-glucagon mRNA detected in fetal rat pancreas. The three antigenic determinants of pancreatic pro-glucagon were co-localized also in intestinal L-cells and in canine gastric A-cells. Canine and feline pancreatic pro-glucagons therefore resemble those identified in other mammals and may also occur in gastrointestinal endocrine cells. Although there is evidence that the GLP I sequence is not liberated from pancreatic pro-glucagon, our results raise the possibility that this putative hormone may be a cleavage product of pro-glucagon in the gastrointestinal tract.

Effect of blood in the gut on measurements of endogenous carbon monoxide production

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 67-69 ◽  
Author(s):  
RP Brouillard ◽  
ME Conrad ◽  
TA Bensinger
Keyword(s):  
Carbon Monoxide ◽  
Gastrointestinal Tract ◽  
Serum Bilirubin ◽  
Porphyrin Ring ◽  
Hemolytic Disease ◽  
Normal Volunteers ◽  
Endogenous Production ◽  
Mucosal Cells ◽  
Intestinal Mucosal Cells

Abstract Changes induced in measurements of endogenous carbon monoxide (CO) production by blood in the lumen of the gut were studied in five normal volunteers. The study was undertaken because exogenous heme is absorbed by intestinal mucosal cells where the porphyrin ring is split with the release of CO that could contribute to blood CO levels and lead to a fallacious diagnosis of hemolytic disease. Volunteers who consumed 200 ml of their own blood doubled their endogenous production of CO (0.69 versus 0.34 mumoles/kg/hr). This suggested that at least 3% of the ingested heme was degraded and recovered as CO within 2 1/2 hr. Measurements of serum bilirubin also showed a significant increase after ingestion of blood. These data indicate that blood in the gastrointestinal tract can interfere with quantification of heme and bilirubin turnover from measurements of either endogenous CO production or bilirubin and suggest that this might occur with the ingestion of meat.

scholarly journals Effect of blood in the gut on measurements of endogenous carbon monoxide production

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 67-69 ◽  
Author(s):  
RP Brouillard ◽  
ME Conrad ◽  
TA Bensinger
Keyword(s):  
Carbon Monoxide ◽  
Gastrointestinal Tract ◽  
Serum Bilirubin ◽  
Porphyrin Ring ◽  
Hemolytic Disease ◽  
Normal Volunteers ◽  
Endogenous Production ◽  
Mucosal Cells ◽  
Intestinal Mucosal Cells

Changes induced in measurements of endogenous carbon monoxide (CO) production by blood in the lumen of the gut were studied in five normal volunteers. The study was undertaken because exogenous heme is absorbed by intestinal mucosal cells where the porphyrin ring is split with the release of CO that could contribute to blood CO levels and lead to a fallacious diagnosis of hemolytic disease. Volunteers who consumed 200 ml of their own blood doubled their endogenous production of CO (0.69 versus 0.34 mumoles/kg/hr). This suggested that at least 3% of the ingested heme was degraded and recovered as CO within 2 1/2 hr. Measurements of serum bilirubin also showed a significant increase after ingestion of blood. These data indicate that blood in the gastrointestinal tract can interfere with quantification of heme and bilirubin turnover from measurements of either endogenous CO production or bilirubin and suggest that this might occur with the ingestion of meat.

The effects of feeding on cell morphology and proliferation of the gastrointestinal tract of juvenile Burmese pythons (Python molurus)

2009 ◽  
Vol 87 (12) ◽  
pp. 1255-1267 ◽  
Author(s):  
Cécile Helmstetter ◽  
Robert K. Pope ◽  
Mathieu T’Flachebba ◽  
Stephen M. Secor ◽  
Jean-Hervé Lignot
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Digestive System ◽  
Cellular Proliferation ◽  
Physiological Changes ◽  
Postprandial Period ◽  
Mucosal Cells ◽  
Python Molurus ◽  
Low Levels ◽  
Cellular Replication

The gastrointestinal tract of Burmese pythons ( Python molurus (L., 1758)) exhibits large morphological and physiological changes in response to feeding and extended periods of fasting. In this study the mucosa of the stomach, small intestine, and colon were examined for changes in structure and cellular proliferation. The mucosa of fasting pythons exhibited low levels of cellular replication, but after feeding, cellular replication was evident as early as 12 h in the small intestine and colon and 24 h in the stomach. Replication peaked 3 days postfeeding for the small intestine and colon, but was still increasing at 6 days postfeeding in the stomach. Interestingly, cell proliferation was still evident after 45 days in the colon. In these tissues, a stock of “ready-to-use” primary lysosomes is found in the mucosal cells of fasting animals, whereas profound intracellular recycling is typical of animals that have been fed. These findings indicate that during the postprandial period, the intestinal mucosa undergoes extensive remodelling in anticipation of the next fasting and feeding period. One key adaptive factor for the python’s ability to cope with infrequent feeding is a well-prepared digestive system in fasting animals that can quickly start functioning again when food becomes available.

scholarly journals The metabolic fate of the amido-N group of glutamine in the tissues of the gastrointestinal tract in 24 h-fasted sheep

1999 ◽  
Vol 81 (4) ◽  
pp. 297-306 ◽  
Author(s):  
J. J. Gate ◽  
D. S. Parker ◽  
G. E. Lobley
Keyword(s):  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Live Weight ◽  
Glutamine Metabolism ◽  
Whole Body ◽  
Total Acid ◽  
Blood Flows ◽  
Mucosal Cells ◽  
Vascular Catheters ◽  
Dna 2

Whole-body and gastrointestinal tract (GIT) metabolism of [5-15N]glutamine were monitored in lambs (33 kg live weight) fasted for 24 h. Animals were previously prepared with vascular catheters across the mesenteric-(MDV) and portal-drained viscera (PDV) to permit quantification of mass and isotopic transfers of metabolites by arterio-venous difference. Continuous infusions of [5-15N]glutamine into the jugular vein were conducted for 10 h and integrated blood samples withdrawn over 75 min intervals for the last 5 h of infusion. The lambs were then killed and portions from various tissues of the digestive tract and other body organs removed for determination of 15N enrichment in RNA, DNA and protein (the latter obtained by difference using total acid-precipitable N). Whole-body glutamine flux was 108 μmol/min of which 23 and 47 % could be attributed to MDV and PDV metabolism (P < 0·001) respectively. There was a small net production of glutamine across the MDV. GIT blood-flows and NH3 production were partitioned 3:2 between MDV and non-MDV components. Less than 5 % of the NH3 produced was derived from the amido-N of glutamine, while across the small intestine (MDV) 26 % of the glutamine flux was converted to NH3, compared with 18 % for non-MDV transfers. The 15N enrichments in protein were of the order jejunum > duodenum > ileum with mucosal cells more labelled than serosal (P < 0·001). Lesser enrichments were observed for other GIT tissues (abomasum > caecum > rumen) while liver and lymph were comparable with the abomasum; kidney, spleen and muscle were lower still (P < 0·05). Enrichments of RNA were similar to that of protein and followed the same pattern, except for higher relative values for liver, spleen and lymphoid tissue. The lowest enrichments were observed for DNA, but again the pattern order was similar except for increased label in lymph, caecum and the spleen. For the MDV there was reasonable agreement between 15N-disappearance as glutamine and appearance in NH3 (24 %), protein (81 %), RNA (3·6 %) and DNA (2·1 %). For the total PDV there was a shortfall (−12 %), however, which may be due to losses in lumen components. These results show the importance of the GIT as a contributor to total glutamine plasma flux, but indicate a lesser reliance on glutamine metabolism by the digestive tract of the ruminant compared with observations from non-ruminants.

Habitat Association of Klebsiella Species

1985 ◽  
Vol 6 (2) ◽  
pp. 52-58 ◽  
Author(s):  
Susan T. Bagley
Keyword(s):  
Drinking Water ◽  
Gastrointestinal Tract ◽  
Surface Waters ◽  
Industrial Effluents ◽  
Opportunistic Pathogen ◽  
Habitat Associations ◽  
Habitat Association ◽  
Klebsiella Species ◽  
Almost All ◽  
Polluted Waters

AbstractThe genus Klebsiella is seemingly ubiquitous in terms of its habitat associations. Klebsiella is a common opportunistic pathogen for humans and other animals, as well as being resident or transient flora (particularly in the gastrointestinal tract). Other habitats include sewage, drinking water, soils, surface waters, industrial effluents, and vegetation. Until recently, almost all these Klebsiella have been identified as one species, ie, K. pneumoniae. However, phenotypic and genotypic studies have shown that “K. pneumoniae” actually consists of at least four species, all with distinct characteristics and habitats. General habitat associations of Klebsiella species are as follows: K. pneumoniae—humans, animals, sewage, and polluted waters and soils; K. oxytoca—frequent association with most habitats; K. terrigena— unpolluted surface waters and soils, drinking water, and vegetation; K. planticola—sewage, polluted surface waters, soils, and vegetation; and K. ozaenae/K. rhinoscleromatis—infrequently detected (primarily with humans).

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