A modified sucrose fractionation procedure for the isolation of frankiae from actinorhizal root nodules and soil samples

1984 ◽  
pp. 23-28
Author(s):  
Dwight Baker ◽  
David O’Keefe
Keyword(s):  
Root Nodules ◽  
Soil Samples ◽  
Fractionation Procedure

scholarly journals Production Bacteriosin by Rhizobia obtained from Root nodules of Macrotyloma uniflorum (Lam.) Verdc. (Horse Gram)

2012 ◽  
Vol 28 (2) ◽  
pp. 76-79
Author(s):  
Prabhavati Edulamudi ◽  
Anthony Johnson Antony Masilamani ◽  
Venkata Ramana Sai Gopal Divi ◽  
Veera Mallaiah Konada
Keyword(s):  
Molecular Weight ◽  
Andhra Pradesh ◽  
Root Nodules ◽  
Inhibition Zone ◽  
Maximum Activity ◽  
Soil Samples ◽  
Horse Gram ◽  
Activity Inhibition ◽  
Macrotyloma Uniflorum ◽  
Nodulation Test

Thirty two Rhizobium isolates obtained from the root nodules of Horse gram plants grown in 32 different soil samples collected from various regions in Andhra Pradesh, India. They were identified as rhizobia by nodulation test. These isolates were used to study the production of bacteriocins. All the isolates produced bacteriocins against the remaining isolates. The isolates HGR-4 and 9 showed maximum activity/inhibition  zone among all the isolates, and also found to be inhibiting more number of isolates than that of other bacteriocin producing isolates. The bacteriocin protein substance produced by the isolates HGR-4 and 9 showed a molecular weight of ~ 50 kDa by SDS-AGE and bacteriocin plasmids molecular weight is > 22 kb and     24 kb. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11820 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 76-79    

LIMITATIONS OF ALKALI-EXTRACTABLE ORGANIC FRACTIONS AS BASES OF SOIL CLASSIFICATION CRITERIA

1984 ◽  
Vol 64 (2) ◽  
pp. 173-186 ◽  
Author(s):  
P. A. SCHUPPLI ◽  
J. A. McKEAGUE
Keyword(s):  
Humic Acid ◽  
Fulvic Acid ◽  
Soil Classification ◽  
Soil Samples ◽  
Classification Criteria ◽  
Acid Fraction ◽  
Fractionation Procedure ◽  
Tenfold Increase ◽  
Organic Fractions ◽  
Humic Acid Carbon

Soil samples, mainly from eastern and northern Canada, were extracted with sodium hydroxide-pyrophosphate and the centrifuged extracts were fractionated into humic acid, fulvic acid and the polyphenolic component of the fulvic acid fraction. Details of the extraction and fractionation procedure were altered systematically and the effects of these changes on amounts of carbon in the various fractions were studied. A tenfold increase in the ratio of soil to extracting solution resulted in a marked increase (nearly double in some cases) in the ratio of humic acid carbon to fulvic acid carbon (Ch/Cf). Increasing the centrifugal force from 1150 × g to 8200 × g had little effect on Ch, but decreased Cf values. Dissolving and reprecipitating humic acid resulted usually in a decrease in Ch/Cf. Different shaking speeds and times, and extraction under nitrogen rather than air had minor, if any, effects on the Ch/Cf ratios. Most of the podzolic B horizons tested could be distinguished from the other 60 horizons by the combination of C ext (extractable carbon), Ch/Cf and Ca/Cf (carbon content of the polyphenols component of the fulvic acid fraction/fulvic acid carbon).Extractable organic fractions have some promise as bases of soil classification criteria but their potential is limited at present. The procedures are time-consuming and exacting, and results are not comparable between laboratories. Rigorous standardization of methods and interlaboratory comparisons of data might lead to more useful criteria based on organic matter composition. Key words: Humic acid carbon, fulvic acid carbon, polyphenols carbon, extractable carbon, classification criteria

scholarly journals Nodulation and Nitrogen-fixing Capacity of Rhizobial Isolates from China in Symbiosis with Maackia amurensis

2007 ◽  
Vol 25 (1) ◽  
pp. 47-50
Author(s):  
James A. Schrader ◽  
Mark Kroggel ◽  
William R. Graves
Keyword(s):  
Root Nodules ◽  
Soil Samples ◽  
Metabolic Efficiency ◽  
Improve Performance ◽  
N Content ◽  
Total N ◽  
Fertilizer Use ◽  
Root Zones ◽  
Maackia Amurensis ◽  
Bradyrhizobium Spp

Abstract Maackia amurensis Rupr. & Maxim (Amur maackia) is a leguminous Asian tree capable of forming N2-fixing symbioses with soil-borne Bradyrhizobium spp. This trait sets Amur maackia apart from many legumes now produced in North American nurseries. Two determinants of N2-fixing capacity in legumes are the compatibility of the host plant and its bacterial microsymbiont and the metabolic efficiency of compatible bacteria. Our objectives were to isolate numerous rhizobia from the root zones of indigenous Amur maackia in China and to select isolates that form superior N2-fixing relationships with inoculated seedlings. Soil samples collected in the Heilongjiang Province of China were used as inocula to establish nodules on seedlings. Putative rhizobia were isolated from these nodules and cultured. Inoculation of additional seedlings with 170 of these isolates evoked nodulation, confirming their identity as rhizobia. Isolates that induced the most nodules were evaluated further. All selected isolates increased growth and total N content of Amur maackia compared to uninoculated controls. Three of the isolates induced more root nodules, and four evoked a higher total N content in plants than did isolate USDA 4349, a previously characterized strain of Bradyrhizobium selected for Amur maackia. Our results demonstrate marked variation among rhizobia compatible with Amur maackia and illustrate the potential to inoculate plants in nurseries and landscapes with superior bacteria, a practice that could reduce fertilizer use and improve performance of trees in N-deficient soils.

scholarly journals Draft Genome Sequences of Nitrogen-Fixing Bradyrhizobia Isolated from Root Nodules of Peanut, Arachis hypogaea, Cultivated in Southern Tunisia

2021 ◽  
Vol 10 (29) ◽  
Author(s):  
Besma Bouznif ◽  
Benoit Alunni ◽  
Mohamed Mars ◽  
Jacqui A. Shykoff ◽  
Tatiana Timchenko ◽  
...  
Keyword(s):  
Arachis Hypogaea ◽  
Root Nodules ◽  
Draft Genome ◽  
Soil Samples ◽  
Nitrogen Fixing ◽  
Genome Sequences ◽  
Southern Tunisia ◽  
South Tunisia

Here, we report the draft genome sequences of two nitrogen-fixing symbionts, Bradyrhizobium sp. strain sGM-13 and Bradyrhizobium sp. strain sBnM-33, isolated from root nodules of peanut grown on soil samples collected from two regions in South Tunisia. The draft genome sizes of these two strains are 8.31 × 106 bp and 8.97 × 106 bp, respectively.

An Evaluation of a Plasma Fractionation System

1960 ◽  
Vol 4 (01) ◽  
pp. 031-044
Author(s):  
George Y. Shinowara ◽  
E. Mary Ruth
Keyword(s):  
Biological Activity ◽  
Ionic Strength ◽  
Plasma Proteins ◽  
High Concentration ◽  
Significant Evidence ◽  
Native Proteins ◽  
Mobility Data ◽  
Fractionation Procedure ◽  
The Individual ◽  
Low Ionic Strength

SummaryFour primary fractions comprising at least 97 per cent of the plasma proteins have been critically appraised for evidence of denaturation arising from a low temperature—low ionic strength fractionation system. The results in addition to those referable to the recovery of mass and biological activity include the following: The high solubilities of these fractions at pH 7.3 and low ionic strengths; the compatibility of the electrophoretic and ultracentrifugal data of the individual fractions with those of the original plasma; and the recovery of hemoglobin, not hematin, in fraction III obtained from specimens contaminated with this pigment. However, the most significant evidence for minimum alterations of native proteins was that the S20, w and the electrophoretic mobility data on the physically recombined fractions were identical to those found on whole plasma.The fractionation procedure examined here quantitatively isolates fibrinogen, prothrombin and antithrombin in primary fractions. Results have been obtained demonstrating its significance in other biological systems. These include the following: The finding of 5 S20, w classes in the 4 primary fractions; the occurrence of more than 90 per cent of the plasma gamma globulins in fraction III; the 98 per cent pure albumin in fraction IV; and, finally, the high concentration of beta lipoproteins in fraction II.

K-MEANS CLUSTERING ALGORITHM BASED CLASSIFICATION OF SOIL FERTILITY IN NORTH WEST NIGERIA

2020 ◽  
Vol 4 (2) ◽  
pp. 780-787
Author(s):  
Ibrahim Hassan Hayatu ◽  
Abdullahi Mohammed ◽  
Barroon Ahmad Isma’eel ◽  
Sahabi Yusuf Ali
Keyword(s):  
Soil Fertility ◽  
Crop Yield ◽  
Clustering Algorithm ◽  
Soil Samples ◽  
North West ◽  
R Programming ◽  
Available Information ◽  
Northwest Region ◽  
The Relationship

Soil fertility determines a plant's development process that guarantees food sufficiency and the security of lives and properties through bumper harvests. The fertility of soil varies according to regions, thereby determining the type of crops to be planted. However, there is no repository or any source of information about the fertility of the soil in any region in Nigeria especially the Northwest of the country. The only available information is soil samples with their attributes which gives little or no information to the average farmer. This has affected crop yield in all the regions, more particularly the Northwest region, thus resulting in lower food production.  Therefore, this study is aimed at classifying soil data based on their fertility in the Northwest region of Nigeria using R programming. Data were obtained from the department of soil science from Ahmadu Bello University, Zaria. The data contain 400 soil samples containing 13 attributes. The relationship between soil attributes was observed based on the data. K-means clustering algorithm was employed in analyzing soil fertility clusters. Four clusters were identified with cluster 1 having the highest fertility, followed by 2 and the fertility decreases with an increasing number of clusters. The identification of the most fertile clusters will guide farmers on where best to concentrate on when planting their crops in order to improve productivity and crop yield.

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