Gap junctions in the rat cochlea: immunohistochemical and ultrastructural analysis

1995 ◽  
Vol 191 (2) ◽  
Author(s):  
Toshihiko Kikuchi ◽  
RobertS. Kimura ◽  
DavidL. Paul ◽  
JoeC. Adams
Keyword(s):  
Gap Junctions ◽  
Ultrastructural Analysis ◽  
Rat Cochlea

scholarly journals Gap Junctions Mediate Glucose Transport Between GLUT1-Positive and -Negative Cells in the Spiral Limbus of the Rat Cochlea

2006 ◽  
Vol 13 (1-2) ◽  
pp. 93-102 ◽  
Author(s):  
Tatsuya Matsunami ◽  
Toshihiro Suzuki ◽  
Yasuo Hisa ◽  
Kuniaki Takata ◽  
Tetsuro Takamatsu ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Glucose Transport ◽  
Spiral Limbus ◽  
Rat Cochlea

scholarly journals Ultrastructural analysis of chemical synapses and gap junctions betweenDrosophila brain neurons in culture

2008 ◽  
Vol 68 (3) ◽  
pp. 281-294 ◽  
Author(s):  
Hyun-Woo Oh ◽  
Jorge M. Campusano ◽  
Lutz G.W. Hilgenberg ◽  
Xicui Sun ◽  
Martin A. Smith ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Ultrastructural Analysis ◽  
Brain Neurons ◽  
Chemical Synapses

Ultrastructural Analysis of Gap Junctions in C6 Glioma Cells Transfected with Connexin43 cDNA

1993 ◽  
Vol 206 (1) ◽  
pp. 72-84 ◽  
Author(s):  
Christian C.G. Naus ◽  
Stephen Hearn ◽  
Daguang Zhu ◽  
Bruce J. Nicholson ◽  
Richard R. Shivers
Keyword(s):  
Gap Junctions ◽  
Glioma Cells ◽  
C6 Glioma ◽  
Ultrastructural Analysis ◽  
C6 Glioma Cells

Roles of gap junctions in glucose transport from glucose transporter 1-positive to -negative cells in the lateral wall of the rat cochlea

2008 ◽  
Vol 131 (1) ◽  
pp. 89-102 ◽  
Author(s):  
Toshihiro Suzuki ◽  
Tatsuya Matsunami ◽  
Yasuo Hisa ◽  
Kuniaki Takata ◽  
Tetsuro Takamatsu ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Glucose Transport ◽  
Glucose Transporter ◽  
Lateral Wall ◽  
Glucose Transporter 1 ◽  
Rat Cochlea

Quantitative EM of gap junction distribution in mutant drosophila wing discs

1983 ◽  
Vol 41 ◽  
pp. 720-721
Author(s):  
J.S. Ryerse
Keyword(s):  
Gap Junctions ◽  
Growth Control ◽  
Intercellular Junctions ◽  
Wing Disc ◽  
En Bloc ◽  
Metabolic Cooperation ◽  
Drosophila Wing ◽  
Adult Wing ◽  
Wing Discs ◽  
Wing Pouch

Gap junctions are intercellular junctions found in both vertebrates and invertebrates through which ions and small molecules can pass. Their distribution in tissues could be of critical importance for ionic coupling or metabolic cooperation between cells or for regulating the intracellular movement of growth control and pattern formation factors. Studies of the distribution of gap junctions in mutants which develop abnormally may shed light upon their role in normal development. I report here the distribution of gap junctions in the wing pouch of 3 Drosophila wing disc mutants, vg (vestigial) a cell death mutant, 1(2)gd (lethal giant disc) a pattern abnormality mutant and 1(2)gl (lethal giant larva) a neoplastic mutant and compare these with wildtype wing discs.The wing pouch (the anlagen of the adult wing blade) of a wild-type wing disc is shown in Fig. 1 and consists of columnar cells (Fig. 5) joined by gap junctions (Fig. 6). 14000x EMs of conventionally processed, UA en bloc stained, longitudinally sectioned wing pouches were enlarged to 45000x with a projector and tracings were made on which the lateral plasma membrane (LPM) and gap junctions were marked.

Structural modifications of intercellular junctions.

1978 ◽  
Vol 36 (2) ◽  
pp. 330-331
Author(s):  
J. Metz ◽  
M. Merlo ◽  
W. G. Forssmann
Keyword(s):  
Gap Junctions ◽  
Intercellular Junctions ◽  
Cell Isolation ◽  
Extrahepatic Cholestasis ◽  
Structural Modifications ◽  
Pancreatic Duct Ligation ◽  
Pancreatic Cells ◽  
Duct Ligation ◽  
Thin Sectioning ◽  
And Function

Structure and function of intercellular junctions were studied under the electronmicroscope using conventional thin sectioning and freeze-etch replicas. Alterations of tight and gap junctions were analyzed 1. of exocrine pancreatic cells under cell isolation conditions and pancreatic duct ligation and 2. of hepatocytes during extrahepatic cholestasis.During the different steps of cell isolation of exocrine pancreatic cells, gradual changes of tight and gap junctions were observed. Tight junctions, which formed belt-like structures around the apex of control acinar cells in situ, subsequently diminished, became interrupted and were concentrated into macular areas (Fig. 1). Aggregations of membrane associated particles, which looked similar to gap junctions, were intermixed within tight junctional areas (Fig. 1). These structures continously disappeared in the last stages of the isolation procedure. The intercellular junctions were finally separated without destroying the integrity of the cell membrane, which was confirmed with porcion yellow, lanthanum chloride and horse radish peroxidase.

Contributions to the mechanisms of mitosis and roles of cytoplasmic microtubules from ultrastructural analyses of fungi

1978 ◽  
Vol 36 (2) ◽  
pp. 266-267
Author(s):  
I. Brent Heath
Keyword(s):  
Nuclear Envelope ◽  
Direct Application ◽  
Ultrastructural Analysis ◽  
General Interest ◽  
Research Results ◽  
Organelle Motility ◽  
Cytoplasmic Microtubules

Detailed ultrastructural analysis of fungal mitotic systems and cytoplasmic microtubules might be expected to contribute to a number of areas of general interest in addition to the direct application to the organisms of study. These areas include possibly fundamental general mechanisms of mitosis; evolution of mitosis; phylogeny of organisms; mechanisms of organelle motility and positioning; characterization of cellular aspects of microtubule properties and polymerization control features. This communication is intended to outline our current research results relating to selected parts of the above questions.Mitosis in the oomycetes Saprolegnia and Thraustotheca has been described previously. These papers described simple kinetochores and showed that the kineto- chores could probably be used as markers for the poorly defined chromosomes. Kineto- chore counts from serially sectioned prophase mitotic nuclei show that kinetochore replication precedes centriole replication to yield a single hemispherical array containing approximately the 4 n number of kinetochore microtubules diverging from the centriole associated "pocket" region of the nuclear envelope (Fig. 1).

Calmodulin-mediated gating of lens gap junction channels in vesicles

1984 ◽  
Vol 42 ◽  
pp. 134-137
Author(s):  
Camillo Peracchia ◽  
Stephen J. Girsch
Keyword(s):  
Gap Junctions ◽  
Freeze Fracture ◽  
Orthogonal Arrays ◽  
Eye Lens ◽  
Lens Fiber ◽  
Cell Coupling ◽  
Particle Spacing ◽  
Fiber Cells ◽  
Gap Junction Channels ◽  
Communicating Junctions

The fiber cells of eye lens communicate directly with each other by exchanging ions, dyes and metabolites. In most tissues this type of communication (cell coupling) is mediated by gap junctions. In the lens, the fiber cells are extensively interconnected by junctions. However, lens junctions, although morphologically similar to gap junctions, differ from them in a number of structural, biochemical and immunological features. Like gap junctions, lens junctions are regions of close cell-to-cell apposition. Unlike gap junctions, however, the extracellular gap is apparently absent in lens junctions, such that their thickness is approximately 2 nm smaller than that of typical gap junctions (Fig. 1,c). In freeze-fracture replicas, the particles of control lens junctions are more loosely packed than those of typical gap junctions (Fig. 1,a) and crystallize, when exposed to uncoupling agents such as Ca++, or H+, into pseudo-hexagonal, rhombic (Fig. 1,b) and orthogonal arrays with a particle-to-particle spacing of 6.5 nm. Because of these differences, questions have been raised about the interpretation of the lens junctions as communicating junctions, in spite of the fact that they are the only junctions interlinking lens fiber cells.

Quantitative Freeze Fracture Studies of Gap Junctions in Somatic Cell Hybrids, Their Parents, and Revertants

1976 ◽  
Vol 34 ◽  
pp. 218-219
Author(s):  
W. J. Larsen ◽  
R. Azarnia ◽  
W. R. Loewenstein
Keyword(s):  
Gap Junctions ◽  
Striated Muscle ◽  
Freeze Fracture ◽  
Cell Movement ◽  
Dye Molecules ◽  
Somatic Cell Hybrids ◽  
Cell Coupling ◽  
Normal Cells ◽  
Mediate Cell ◽  
Almost All

Although the physiological significance of the gap junction remains unspecified, these membrane specializations are now recognized as common to almost all normal cells (excluding adult striated muscle and some nerve cells) and are found in organisms ranging from the coelenterates to man. Since it appears likely that these structures mediate the cell-to-cell movement of ions and small dye molecules in some electrical tissues, we undertook this study with the objective of determining whether gap junctions in inexcitable tissues also mediate cell-to-cell coupling.To test this hypothesis, a coupling, human Lesh-Nyhan (LN) cell was fused with a non-coupling, mouse cl-1D cell, and the hybrids, revertants, and parental cells were analysed for coupling with respect both to ions and fluorescein and for membrane junctions with the freeze fracture technique.

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