Microbial colonization of different support materials used to enhance the methanogenic process

1994 ◽  
Vol 41 (4) ◽  
pp. 480-486 ◽  
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego
1994 ◽  
Vol 41 (4) ◽  
pp. 480-486
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego

1994 ◽  
Vol 41 (4) ◽  
pp. 480-486 ◽  
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego

Author(s):  
Dr. Samuel Nyaga Jesse ◽  
Prof. John Nyamai Maundu ◽  
Prof. Nicholas Wanjala Twoli

The curriculum support materials used in an instructional process may influence assessment and achievement of learners. Curriculum support materials are both print and electronic in nature. One of the main electronic curriculum support materials used in schools is interactive digital content. In recent years, there has been rapid expansion in curriculum digital content access and utilization in Kenyan schools. However, little has been done to establish how utilization of curriculum digital content in the instructional process influences learners’ assessment and achievement. The study reported in this paper therefore investigated the influence of curriculum digital content utilisation on secondary school learners’ assessment and achievement in Biology. It was guided by two specific objectives including to: investigate the influence of the utilisation of Biology digital content on learners’ assessment and examine the influence of the utilisation of Biology digital content on learners’ achievement. A mixed methods approach that employed triangulation design was used in this study. Data was obtained from fifteen Economic Stimulus Programme-ICT phase 1 secondary schools in Nairobi County. Three instruments including Biology teacher questionnaire, learners’ focus group discussion guide and documents analysis sheet were used for data collection. The collected data was analyzed using both descriptive and inferential statistics and presented in form of notes, tables, and graphics. This study found out that most assessment aspects improved with curriculum digital content utilization. In addition, utilisation of curriculum digital content had a positive influence on the achievement of secondary school learners in Biology. The study recommended that all schools, and not only ESP-ICT schools, be provided with proper infrastructure to enable learners and teachers access and utilize curriculum digital content in the instructional process. This would enhance assessment modes and learners achievement in secondary schools in Kenya.


2016 ◽  
Vol 17 (3) ◽  
pp. 293 ◽  
Author(s):  
Lorenzo Drago ◽  
Serse Agrappi ◽  
Monica Bortolin ◽  
Marco Toscano ◽  
Carlo Romanò ◽  
...  

2017 ◽  
Vol 262 ◽  
pp. 682-686 ◽  
Author(s):  
Letícia Ferraresi Hidalgo ◽  
Jessyka Lima Santos ◽  
Samir Prioto Tayar ◽  
Arnaldo Sarti ◽  
Mauricio Cesar Palmieri ◽  
...  

Biogas is a renewable energy source that can be used to produce heat and energy, replacing fossil fuels. The main factor limiting the use of biogas is contaminants in its composition which H2S is the most important due to corrosion and environmental problems. A promising technology to remove this contaminant from biogas is the biotrickling filters and the selection of inexpensive and durable supports is an important step for the operation. This work has studied different support materials, for microorganisms immobilization, as Polyvinyl Chloride (PVC), Polyethylene Terephtalate (PET), Polytetrafluoroethylene (Teflon®) comparing to open pore polyurethane foam (OPP) each one packed in biotrickling filters to evaluate the consumption of thiosulfate by chemolitotrofic microorganisms. The kinetics of substrate consumption in different cycles for each support were distinct suggesting different microbial colonization. The materials tested have presented results very similar polyurethane foam, which has already known by its efficiency on biogas desulfurization, unless Teflon that has showed a divergent result with the increase of the substrate concentration in the system.


Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 801
Author(s):  
Milena Marycz ◽  
Anna Brillowska-Dąbrowska ◽  
Jacek Gębicki

The paper describes the process of n-butanol abatement by unicellular fungi, able to deplete n-butanol content in gas, by using n-butanol as source of carbon. Isolated and identified fungi species Candida albicans and Candida subhashii were subjected to a viability process via assimilation of carbon from hydrophilic and hydrophobic compounds. The isolates, which exhibited the ability to assimilate carbon, were immobilized on four different types of artificial support materials used for biotrickling filtration. Application of optical microscopy, flow cytometry and the tests employing propidium iodide and annexin V revealed viability of the fungi isolated on support materials’ surfaces at the average level of 95%. The proposed method of immobilization and its evaluation appeared to be effective, cheap and fast. Based on performed comparative analyses, it was shown that polyurethane foam and Bialecki rings (25 × 25) could be attractive support materials in biotrickling filtration.


Microbiome ◽  
2022 ◽  
Vol 10 (1) ◽  
Author(s):  
Kaela K. Amundson ◽  
Mikayla A. Borton ◽  
Rebecca A. Daly ◽  
David W. Hoyt ◽  
Allison Wong ◽  
...  

Abstract Background Microbial colonization of subsurface shales following hydraulic fracturing offers the opportunity to study coupled biotic and abiotic factors that impact microbial persistence in engineered deep subsurface ecosystems. Shale formations underly much of the continental USA and display geographically distinct gradients in temperature and salinity. Complementing studies performed in eastern USA shales that contain brine-like fluids, here we coupled metagenomic and metabolomic approaches to develop the first genome-level insights into ecosystem colonization and microbial community interactions in a lower-salinity, but high-temperature western USA shale formation. Results We collected materials used during the hydraulic fracturing process (i.e., chemicals, drill muds) paired with temporal sampling of water produced from three different hydraulically fractured wells in the STACK (Sooner Trend Anadarko Basin, Canadian and Kingfisher) shale play in OK, USA. Relative to other shale formations, our metagenomic and metabolomic analyses revealed an expanded taxonomic and metabolic diversity of microorganisms that colonize and persist in fractured shales. Importantly, temporal sampling across all three hydraulic fracturing wells traced the degradation of complex polymers from the hydraulic fracturing process to the production and consumption of organic acids that support sulfate- and thiosulfate-reducing bacteria. Furthermore, we identified 5587 viral genomes and linked many of these to the dominant, colonizing microorganisms, demonstrating the key role that viral predation plays in community dynamics within this closed, engineered system. Lastly, top-side audit sampling of different source materials enabled genome-resolved source tracking, revealing the likely sources of many key colonizing and persisting taxa in these ecosystems. Conclusions These findings highlight the importance of resource utilization and resistance to viral predation as key traits that enable specific microbial taxa to persist across fractured shale ecosystems. We also demonstrate the importance of materials used in the hydraulic fracturing process as both a source of persisting shale microorganisms and organic substrates that likely aid in sustaining the microbial community. Moreover, we showed that different physicochemical conditions (i.e., salinity, temperature) can influence the composition and functional potential of persisting microbial communities in shale ecosystems. Together, these results expand our knowledge of microbial life in deep subsurface shales and have important ramifications for management and treatment of microbial biomass in hydraulically fractured wells.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Benjamín Valdez-Salas ◽  
Ernesto Beltrán-Partida

Ti6Al4V alloys are the primary materials used for clinical bone regeneration and restoration; however, they are substantially susceptible to biomaterial-related infections. Therefore, in the present work, we applied a controllable and stable oxidative nanopatterning strategy by applying H3PO4, a weaker dissociating acid, as a substitute for H2SO4 in the classical piranha reaction. The results suggest that our method acted as a concomitant platform to develop reproducible diameter-controlled TiO2 nanopores (NPs). Interestingly, our procedure illustrated stable temperature reactions without exothermic responses since the addition of mixture preparation to the nanopatterning reactions. The reactions were carried out for 30 min (NP14), 1 h (NP7), and 2 h (NP36), suggesting the formation of a thin nanopore layer as observed by Raman spectroscopy. Moreover, the antimicrobial activity revealed that NP7 could disrupt active microbial colonization for 2 h and 6 h. The phenotype configuration strikingly showed that NP7 does not alter the cell morphology, thus proposing a disruptive adhesion pathway instead of cellular lysis. Furthermore, preliminary assays suggested an early promoted osteoblasts viability in comparison to the control material. Our work opens a new path for the rationale design of nanobiomaterials with “intelligent surfaces” capable of decreasing microbial adhesion, increasing osteoblast viability, and being scalable for industrial transfer.


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