Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

1978 ◽  
Vol 52 (3) ◽  
pp. 135-139 ◽  
Author(s):  
J. C. Snyder ◽  
S. L. Desborough
Keyword(s):  
Potato Tuber ◽  
Protein Content ◽  
Total Protein ◽  
Brilliant Blue ◽  
Total Protein Content ◽  
Brilliant Blue G ◽  
Rapid Estimation ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

A comparative study of the protein content of some helminths and the suitability of assay methods

1991 ◽  
Vol 65 (1) ◽  
pp. 62-66
Author(s):  
S. M. A. Abidi ◽  
W. A. Nizami
Keyword(s):  
Comparative Study ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Tissue Homogenates ◽  
Assay Methods ◽  
Phenol Reagent ◽  
Coomassie Brilliant

ABSTRACTThe protein content of fresh homogenates and their corresponding TCA precipitated fractions of 10 different species of helminths was estimated by the methods of Lowry et al. and Spector using the Folin phenol reagent and Coomassie brilliant blue G-250 respectively. The former method gives exaggerated values as compared to the latter method. The parasite phenols, phenolic proteins and catecholamines could be responsible for interference in the Lowry's procedure. The TCA non-precipitable moieties also give colour only with the Folin phenol assay. The pronounced intra-specific differences in the total protein content of helminths reflect their metabolic variations and adaptations. Habitat does not appear to influence the protein content of parasites, however, the effect of host variation was evident in the pouched amphistome G. crumenifer. It is concluded that the dye binding method gives more consistent results and it can be conveniently applied to crude tissue homogenates of helminths.

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

1989 ◽  
Vol 35 (10) ◽  
pp. 2127-2129 ◽  
Author(s):  
Y Bergqvist ◽  
L Karlsson ◽  
L Fohlin
Keyword(s):  
Breast Milk ◽  
Total Protein ◽  
Brilliant Blue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Simple Method ◽  
Coomassie Brilliant Blue ◽  
Kjeldahl Method ◽  
Coomassie Brilliant

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

Automatic assay of urinary protein using Coomassie Brilliant Blue G-250

1981 ◽  
Vol 113 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Kiyoko Sano ◽  
Kiyoko Kanamori ◽  
Akihiko Shiba ◽  
Makoto Nakao
Keyword(s):  
Urinary Protein ◽  
Brilliant Blue ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant
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