An alkaline sucrose gradient analysis of the mechanism of nuclear DNA synthesis in the yeast Saccharomyces cerevisiae

1978 ◽  
Vol 164 (2) ◽  
pp. 217-225 ◽  
Author(s):  
L. H. Johnston ◽  
D. H. Williamson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Synthesis ◽  
Sucrose Gradient ◽  
Gradient Analysis ◽  
Nuclear Dna ◽  
Yeast Saccharomyces Cerevisiae

On the mechanism of premeiotic DNA synthesis in the yeast Saccharomyces cerevisiae

1982 ◽  
Vol 141 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Leland H. Johnston ◽  
Donald H. Williamson ◽  
Anthony L. Johnson ◽  
Daphne J. Fennell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Synthesis ◽  
Yeast Saccharomyces Cerevisiae

Protection of nuclear DNA by lifespan-extending compounds in the yeast Saccharomyces cerevisiae

2021 ◽  
Vol 822 ◽  
pp. 111738
Author(s):  
Wei-Hsuan Su ◽  
Christelle E.T. Chan ◽  
Ting Lian ◽  
Mareena Biju ◽  
Ayaka Miura ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Dna ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Defective processing of ribosomal precursor RNA in Saccharomyces cerevisiae

1984 ◽  
Vol 220 (2) ◽  
pp. 461-467 ◽  
Author(s):  
J A Mitlin ◽  
M Cannon
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Strain ◽  
Ribosome Biogenesis ◽  
Sucrose Gradient ◽  
Gradient Analysis ◽  
Ribosomal Subunits ◽  
Abnormal Distribution ◽  
Precursor Rna ◽  
Ribosomal Precursor

Saccharomyces cerevisiae (strain A224A) has an abnormal distribution of cytoplasmic ribosomal subunits when grown at 36 degrees C, with sucrose-gradient analysis of extracts revealing an apparent excess of material sedimenting at 60 S. This abnormality is not observed at either 23 degrees C or 30 degrees C. At 36 degrees C the defect(s) is expressed as a slowed conversion of 20 S ribosomal precursor RNA to mature 18 S rRNA, although the corresponding maturation of 27 S ribosomal precursor RNA to mature 25 S rRNA is normal. Studies on this yeast strain and on mutants derived from it may help to elucidate the role(s) of individual ribosomal components in controlling ribosome biogenesis in eukaryotes.

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