Nucleotide sequence of the recognition site of the B-specific restriction modification system in E. coli

1979 ◽  
Vol 168 (3) ◽  
pp. 331-335 ◽  
Author(s):  
Reinhold Sommer ◽  
Heinz Schaller
Keyword(s):  
Nucleotide Sequence ◽  
Recognition Site ◽  
Modification System ◽  
E Coli ◽  
Restriction Modification System ◽  
Specific Restriction ◽  
Restriction Modification

A novel plasmid-mediated DNA restriction-modification system in E. coli

Plasmid ◽  
1980 ◽  
Vol 4 (3) ◽  
pp. 350-351 ◽  
Author(s):  
L.I. Glatman ◽  
A.F. Moroz ◽  
M.B. Yablokova ◽  
B.A. Rebentish ◽  
G.V. Kc̵holmina
Keyword(s):  
Modification System ◽  
E Coli ◽  
Restriction Modification System ◽  
Dna Restriction ◽  
Restriction Modification

scholarly journals Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts

1991 ◽  
Vol 19 (4) ◽  
pp. 841-850 ◽  
Author(s):  
Joan E. Brooks ◽  
Peter D. Nathan ◽  
David Landry ◽  
Laura A. Sznyter ◽  
Phyllis Waite-Rees ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals Nucleotide sequence of thePaeR7 restriction/modification system and partial characterization of its protein products

1985 ◽  
Vol 13 (23) ◽  
pp. 8441-8461 ◽  
Author(s):  
G. Theriault ◽  
P.H. Roy ◽  
K.A. Howard ◽  
J.S. Benner ◽  
J.E. Brooks ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Partial Characterization ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

Molecular cloning and expression of NlaIII restriction-modification system in E. coli

Gene ◽  
1996 ◽  
Vol 183 (1-2) ◽  
pp. 215-218 ◽  
Author(s):  
Richard D. Morgan ◽  
Russell R. Camp ◽  
Geoffrey G. Wilson ◽  
Shuang-yong Xu
Keyword(s):  
Molecular Cloning ◽  
Cloning And Expression ◽  
Modification System ◽  
E Coli ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals The Influence of 5′R and 5′S cdA and cdG on the Activity of BsmAI and SspI Restriction Enzymes

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3750
Author(s):  
Michał Szewczuk ◽  
Karolina Boguszewska ◽  
Julia Kaźmierczak-Barańska ◽  
Bolesław T. Karwowski
Keyword(s):  
Genetic Material ◽  
Restriction Enzymes ◽  
Dna Lesions ◽  
Recognition Site ◽  
Recognition Sequence ◽  
Cellular Mechanisms ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification ◽  
Oligonucleotide Sequence

Restriction endonucleases (REs) are intra-bacterial scissors that are considered tools in the fight against foreign genetic material. SspI and BsmAI, examined in this study, cleave dsDNA at their site of recognition or within a short distance of it. Both enzymes are representatives of type II REs, which have played an extremely important role in research on the genetics of organisms and molecular biology. Therefore, the study of agents affecting their activity has become highly important. Ionizing radiation may damage basic cellular mechanisms by inducing lesions in the genome, with 5′,8-cyclo-2′-deoxypurines (cdPus) as a model example. Since cdPus may become components of clustered DNA lesions (CDLs), which are unfavorable for DNA repair pathways, their impact on other cellular mechanisms is worthy of attention. This study investigated the influence of cdPus on the elements of the bacterial restriction–modification system. In this study, it was shown that cdPus present in DNA affect the activity of REs. SspI was blocked by any cdPu lesion present at the enzyme’s recognition site. When lesions were placed near the recognition sequence, the SspI was inhibited up to 46%. Moreover, (5′S)-5′,8-cyclo-2′-deoxyadenosine (ScdA) present in the oligonucleotide sequence lowered BsmAI activity more than (5′R)-5′,8-cyclo-2′-deoxyadenosine (RcdA). Interestingly, in the case of 5′,8-cyclo-2′-deoxyguanosine (cdG), both 5′S and 5′R diastereomers inhibited BsmAI activity (up to 55% more than cdA). The inhibition was weaker when cdG was present at the recognition site rather than the cleavage site.

scholarly journals Characterization of Plasmid pPO1 from the HyperacidophilePicrophilus oshimae

Archaea ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Angel Angelov ◽  
Jörn Voss ◽  
Wolfgang Liebl
Keyword(s):  
Ph Optimum ◽  
Modification System ◽  
Moderately Thermophilic ◽  
E Coli ◽  
Restriction Modification System ◽  
Picrophilus Torridus ◽  
Transcriptional Start Sites ◽  
Native Host ◽  
Restriction Modification

Picrophilus oshimaeandPicrophilus torridusare free-living, moderately thermophilic and acidophilic organisms from the lineage ofEuryarchaeota. With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known. So far, nothing is known about plasmid biology in these hyperacidophiles. Also, there are no genetic tools available for this genus. We have mobilized the 7.6 Kbp plasmid fromP. oshimaeinE. coliby introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy number in the native host, mode of replication, and transcriptional start sites of the encoded ORFs. Plasmid pPO1 may encode a restriction/modification system in addition to its replication functions. The information gained from the pPO1 plasmid may prove useful in developing a cloning system for this group of extreme acidophiles.

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