Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe

1981 ◽  
Vol 182 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Kim Nasmyth ◽  
Paul Nurse
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Division Cycle

Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe

1976 ◽  
Vol 146 (2) ◽  
pp. 167-178 ◽  
Author(s):  
Paul Nurse ◽  
Pierre Thuriaux ◽  
Kim Nasmyth
Keyword(s):  
Cell Division ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Genetic Control ◽  
Cell Division Cycle

scholarly journals Schizosaccharomyces pombe Noc3 Is Essential for Ribosome Biogenesis and Cell Division but Not DNA Replication

2008 ◽  
Vol 7 (9) ◽  
pp. 1433-1440 ◽  
Author(s):  
Christopher R. Houchens ◽  
Audrey Perreault ◽  
François Bachand ◽  
Thomas J. Kelly
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Ribosome Biogenesis ◽  
Budding Yeast ◽  
Ribosomal Subunit ◽  
Dna Helicase ◽  
Yeast Cells ◽  
Direct Role

ABSTRACT The initiation of eukaryotic DNA replication is preceded by the assembly of prereplication complexes (pre-RCs) at chromosomal origins of DNA replication. Pre-RC assembly requires the essential DNA replication proteins ORC, Cdc6, and Cdt1 to load the MCM DNA helicase onto chromatin. Saccharomyces cerevisiae Noc3 (ScNoc3), an evolutionarily conserved protein originally implicated in 60S ribosomal subunit trafficking, has been proposed to be an essential regulator of DNA replication that plays a direct role during pre-RC formation in budding yeast. We have cloned Schizosaccharomyces pombe noc3 + (Spnoc3 +), the S. pombe homolog of the budding yeast ScNOC3 gene, and functionally characterized the requirement for the SpNoc3 protein during ribosome biogenesis, cell cycle progression, and DNA replication in fission yeast. We showed that fission yeast SpNoc3 is a functional homolog of budding yeast ScNoc3 that is essential for cell viability and ribosome biogenesis. We also showed that SpNoc3 is required for the normal completion of cell division in fission yeast. However, in contrast to the proposal that ScNoc3 plays an essential role during DNA replication in budding yeast, we demonstrated that fission yeast cells do enter and complete S phase in the absence of SpNoc3, suggesting that SpNoc3 is not essential for DNA replication in fission yeast.

scholarly journals Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pombe.

1979 ◽  
Vol 137 (1) ◽  
pp. 440-446 ◽  
Author(s):  
M Minet ◽  
P Nurse ◽  
P Thuriaux ◽  
J M Mitchison
Keyword(s):  
Cell Division ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Cell Division Cycle ◽  
A Cell

The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe

1988 ◽  
Vol 89 (3) ◽  
pp. 343-357 ◽  
Author(s):  
I.M. Hagan ◽  
J.S. Hyams
Keyword(s):  
Cell Division ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Cell Division Cycle ◽  
Microtubule Organization ◽  
Cytoplasmic Face ◽  
Cytoplasmic Microtubules ◽  
Positional Control

We have characterized the changes in microtubule organization that occur through the cell division cycle of the fission yeast Schizosaccharomyces pombe by indirect immunofluorescence microscopy. During interphase, groups of cytoplasmic microtubules, independent of the spindle pole body (SPB), form an array extending between the cell tips. These microtubules are involved in positioning the nucleus at the cell equator and in the establishment of cell polarity. At mitosis, the interphase array disappears and is replaced by an intranuclear spindle extending between the now duplicated SPBs. Elongation of the spindle sees the appearance of astral microtubules emanating from the cytoplasmic face of the SPBs. These persist until the end of anaphase whereupon the spindle microtubules depolymerize and two microtubule organizing centres (MTOCs) at the cell equator re-establish the interphase array. We have used the unique properties of various cell division cycle mutants to investigate further the function of these different microtubule arrays and their temporal and positional control.

scholarly journals Mathematical model of the cell division cycle of fission yeast

2001 ◽  
Vol 11 (1) ◽  
pp. 277 ◽  
Author(s):  
Bela Novak ◽  
Zsuzsa Pataki ◽  
Andrea Ciliberto ◽  
John J. Tyson
Keyword(s):  
Mathematical Model ◽  
Cell Division ◽  
Fission Yeast ◽  
Cell Division Cycle

scholarly journals DNA polymerase ε encoded by cdc20 + is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe

1998 ◽  
Vol 3 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Akio Sugino ◽  
Takeshi Ohara ◽  
Josef Sebastian ◽  
Naomi Nakashima ◽  
Hiroyuki Araki
Keyword(s):  
Dna Replication ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Dna Polymerase ◽  
Chromosomal Dna

Overexpression Phenotypes of Plk1 and Ndrg2 in Schizosaccharomyces Pombe: Fission Yeast System for Mammalian Gene Study

2005 ◽  
Vol 277-279 ◽  
pp. 1-6 ◽  
Author(s):  
Young Joo Jang ◽  
Young Sook Kil ◽  
Jee Hee Ahn ◽  
Jae Hoon Ji ◽  
Jong Seok Lim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Rna Splicing ◽  
Mammalian Cells ◽  
Protein Modification ◽  
Genetic System ◽  
Functional Study ◽  
Mammalian Genes

The fission yeast, Schizosaccharomyces pombe is a single-celled free-living fungus that shares many features with cells of more complicated eukaryotes. Many of the genes required for the cell-cycle control, proteolysis, protein modification, and RNA splicing are highly conserved with those of higher eukaryotes. Moreover, fission yeast has the merit of genetics and its genetic system is already well characterized. As such, the current study evaluated the use of a fission yeast system as a tool for the functional study of mammalian genes and attempted to set up an assay system for novel genes. Since the phenotypes of a deletion mutant and the overexpression of a gene are generally analyzed for a functional study of specific genes in yeast, the present study used overexpression phenotypes to study the functions of mammalian genes. Therefore, based on using a thiamine-repressive promoter, two mammalian genes were expressed in fission yeast, and their overexpressed phenotypes compared with those in mammalian cells. The phenotypes resulting from overexpression were analyzed using a FACS, which analyzes the DNA contents, and a microscope. One of the selected genes was the mammalian Polo-like kinase 1 (Plk1), which is activated and plays a role in the mitotic phase of the cell division cycle. The overexpression of various constructs of Plk1 in the HeLa cells caused cell cycle defects, suggesting that the ectopic Plk1s blocked the endogenous Plk1 in the cells. As expected, when the constructs were overexpressed in the fission yeast system, the cells were arrested in mitosis and defected at the end of mitosis. As such, this data suggests that the Plk1-overexpressed phenotypes were similar in the mammalian cells and the fission yeast, thereby enabling the mammalian Plk1 functions to be approximated in the fission yeast. The other selected gene was the N-Myc downstream-regulated gene 2 (ndrg2), which is upregulated during cell differentiation, yet still not well characterized. When the ndrg2 gene was overexpressed in the fission yeast, the cells contained multi-septa. The septa were positioned well, yet their number increased per cell. Therefore, this gene was speculated to block cell division in the last stage of the cell cycle, making the phenotype potentially useful for explaining cell growth and differentiation in mammalian cells. Accordingly, fission yeast is demonstrated to be an appropriate species for the functional study of mammalian genes.

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