Genome exclusion and two strategies of chromosome duplication in oogenesis of a hybrid frog

1991 ◽  
Vol 78 (1) ◽  
pp. 32-34 ◽  
Author(s):  
H. G. Tunner ◽  
S. Heppich-Tunner
Keyword(s):  
Chromosome Duplication ◽  
Genome Exclusion

scholarly journals Chromosome doubling in Cattleya tigrina A. Rich

2019 ◽  
Vol 15 (11) ◽  
Author(s):  
Thays Saynara Alves Menezes-Sá ◽  
Maria de Fátima Arrigoni-Blank ◽  
Andréa Santos da Costa ◽  
Janay De Almeida Santos-Serejo ◽  
Arie Fitzgerald Blank ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Propidium Iodide ◽  
Chromosome Doubling ◽  
Flow Cytometric Analysis ◽  
Leaf Tissue ◽  
Stomatal Index ◽  
Chromosome Duplication ◽  
Flow Cytometric ◽  
Young Leaves ◽  
Commercial Value

Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and fragrance when compared with diploid orchids. This work aimed to induce and confirm artificial polyploidization, using flow cytometry and stomatal analysis. Explants were treated with colchicine at concentrations of 0, 2.5, 7.5, and 12.5 mM, for 24 and 48 hours and with oryzalin, at concentrations of 0, 10, 30, and 50 μM, for three and six days. For the flow cytometric analysis, a sample of leaf tissue was removed from each plant, crushed to release the nuclei and stained with propidium iodide. In addition to flow cytometry, the ploidy of the antimitotic treated plants was evaluated by stomata analysis. Young leaves were used where the density, functionality and stomatal index were evaluated. Colchicine provided induction of satisfactory polyploidy in C. tigrina at all concentrations and times of exposure, obtaining a greater number of polyploid individuals in the concentration of 12.5 mM for 48 hours. Oryzalin did not induce chromosome duplication at the tested concentrations.

Observations on chromosome duplication in cultured human leucocytes

1963 ◽  
Vol 31 (1) ◽  
pp. 100-112 ◽  
Author(s):  
S. Bader ◽  
O.J. Miller ◽  
B.B. Mukherjee
Keyword(s):  
Chromosome Duplication

Synchrony of Chromosome Duplication

Nature ◽  
1966 ◽  
Vol 209 (5022) ◽  
pp. 537-538 ◽  
Author(s):  
C. W. GILBERT ◽  
L. G. LAJTHA ◽  
S. MULDAL ◽  
C. H. OCKEY
Keyword(s):  
Chromosome Duplication

scholarly journals Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting

DNA Repair ◽  
2014 ◽  
Vol 22 ◽  
pp. 12-23 ◽  
Author(s):  
Anamarija Štafa ◽  
Marina Miklenić ◽  
Bojan Žunar ◽  
Berislav Lisnić ◽  
Lorraine S. Symington ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Chromosome Duplication

scholarly journals Is there "Metabolic" DNA in the Mouse Seminal Vesicle?

1960 ◽  
Vol 7 (4) ◽  
pp. 657-666 ◽  
Author(s):  
Joseph G. Gall ◽  
William W. Johnson
Keyword(s):  
Dna Synthesis ◽  
Seminal Vesicle ◽  
Intraperitoneal Injection ◽  
Mitotic Rate ◽  
Thymidine Uptake ◽  
Metabolic Function ◽  
Unimodal Distribution ◽  
Chromosome Duplication ◽  
Silver Grains

This study was designed to answer the question: Is H3-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H3-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered with autoradiographic stripping film. The silver grains above labeled nuclei were counted, and the Feulgen dye contents of these same nuclei were determined photometrically after removal of the grains from the emulsion. Unlabeled nuclei were also measured. The dye contents of non-radioactive nuclei form a unimodal distribution, indicating that polyploidy is absent from this tissue. The radioactive nuclei fall into two groups. In the first, the average dye content is the same as that of the cold nuclei (2C). In the second, the values range from 2C to 4C. In the 2C to 4C group the grain count is proportional to the dye content, showing that incorporation is correlated with synthesis. The radioactive 2C nuclei arose by mitosis during the course of the experiment. This is shown by the following facts: (1) They frequently occur in pairs. (2) They average smaller than unlabeled 2C nuclei. (3) Their average grain count is approximately half that of the 4C nuclei. (4) Labeled division figures are found. (5) A mitotic rate estimated from the number of labeled 2C nuclei accords reasonably well with one based on the number of observed mitoses. Since the incorporation of thymidine accompanies DNA synthesis and precedes mitosis, there is no reason to postulate a special "metabolic" DNA in this tissue.

scholarly journals Verification and characterization of chromosome duplication in haploid maize

2015 ◽  
Vol 14 (2) ◽  
pp. 6999-7007 ◽  
Author(s):  
E.G. de Oliveira Couto ◽  
E.V. Resende Von Pinho ◽  
R.G. Von Pinho ◽  
A.D. Veiga ◽  
M.R. de Carvalho ◽  
...  
Keyword(s):  
Chromosome Duplication
Sign in / Sign up

Export Citation Format

Share Document