A carrier cell line of measles virus in Lu 106 cells

1967 ◽  
Vol 20 (2) ◽  
pp. 215-224 ◽  
Author(s):  
E. Norrby
Keyword(s):  
Nature ◽  
1983 ◽  
Vol 305 (5930) ◽  
pp. 153-155 ◽  
Author(s):  
Michael J. Carter ◽  
Margaret M. Willcocks ◽  
Volker ter Meulen

2000 ◽  
Vol 8 (4) ◽  
pp. 1-6
Author(s):  
Timothy Ryken ◽  
Bruce Frankel ◽  
Sharon Longo ◽  
Zita Sibenaller

Object An experimental model of the fas and fas ligand (fasL) interaction in malignant glioma was developed. Methods Using plasmid-based delivery, 36B10 rat glioma cells were modified to express fas (36B10-fas), and a delivery fibroblast cell line was modified to produce fasL, resulting in the FR-fasL cell line. Evaluation of fas expression was performed with flow cytometry and expression of fasL confirmed by Western blot analysis. Once the cell lines were created and partially characterized, fas-induced cytotoxicity was evaluated using an antibody-mediated assay for 36B10-fas that demonstrated significant toxicity at 24 and 48 hours. To evaluate the potential for activating the fas molecule by using cell-mediated delivery, coculture cytotoxicity studies were performed with a target cell line (36B10-fas) and effector cell line (FR-fasL). Using a series of culture ratios, increasing cytotoxicity was noted, suggesting that activation of the transfected fas receptor by fasL expression on the carrier cell was occurring. Conclusion Based on their experiments, the authors describe a model for evaluating the interaction of fas and fasL in a cellular model of malignant glioma.


1989 ◽  
Vol 24 (3) ◽  
pp. 313-320
Author(s):  
Barry Ziola ◽  
Michael Morhart ◽  
Lynn Gilbert ◽  
Brenda Karvonen ◽  
Xiao Ping Chen

1965 ◽  
Vol 11 (3) ◽  
pp. 435-439 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau

BS-C-1 cells, cultivated in a simple medium designated M-E, were found to be suitable for a plaque assay of polio and measles viruses. In the plaque assay both low- and high-passage levels of the BS-C-1 cell line were highly susceptible to measles virus but titers of polioviruses were lower in the high-passage cells. Low-passage cells, preserved and stored at −196 °C for prolonged periods, are recommended for the titration of polio and measles viruses.


1963 ◽  
Vol 9 (3) ◽  
pp. 417-420 ◽  
Author(s):  
W. Yarosh ◽  
R. E. Armstrong
Keyword(s):  

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