The relationship of the (Na++K+)-activated enzyme system to transport of sodium and potassium across the cell membrane

1973 ◽  
Vol 4 (1-2) ◽  
pp. 1-30 ◽  
Author(s):  
J. C. Skou
Keyword(s):  
Cell Membrane ◽  
Enzyme System ◽  
Relationship Of ◽  
The Relationship ◽  
Sodium And Potassium

scholarly journals Effects of Hypertonic Stress on The Fine Structure of Sickled Erythrocytes

Blood ◽  
1970 ◽  
Vol 35 (4) ◽  
pp. 437-446 ◽  
Author(s):  
JAMES G. WHITE
Keyword(s):  
Fine Structure ◽  
Cell Membrane ◽  
Characteristic Feature ◽  
Dominant Factor ◽  
Hypertonic Stress ◽  
Erythrocyte Sickling ◽  
Relationship Of ◽  
The Relationship

Abstract The tendency of polymers of sickled hemoglobin (HbS) to align parallel and equidistant to each other has been pointed out in many investigations, and is considered a characteristic feature of erythrocyte sickling. A previous study on stroma-free solutions of sickled hemoglobin, however, suggested that polymers of HbS preferentially assumed radial rather than parallel relationships. Sickled erythrocytes were exposed to hypertonic stress in the present study in order to observe whether parallel bundles of polymers remained intact after removal of the cell membrane. Bundles of polymers in salicylate damaged sickled cells regularly developed branching and radial configurations similar to those found in stroma-free gels. Rotational stress appears to be the dominant factor influencing the relationship of HbS polymers, and the force generated by that tension may be an important factor in erythrocyte sickling.

Isolation and characterization of the components of the sodium pump

1974 ◽  
Vol 7 (2) ◽  
pp. 239-274 ◽  
Author(s):  
Peter Leth Jørgensen
Keyword(s):  
Cell Membrane ◽  
Active Transport ◽  
Transport System ◽  
Sodium Pump ◽  
Enzyme System ◽  
Intact Cell ◽  
Test Tube ◽  
Isolation And Characterization ◽  
Sodium And Potassium

A satisfactory understanding of the functions of the sodium pump, the system responsible for the active transport of sodium and potassium, require the isolation and characterization of its protein and lipid components which are integrated in the structure of the cell membrane. The enzyme system (Na+ + K+)-ATPase, is located in membrane fragments and behaves in the test tube like the transport system in the intact cell membrane (Skou,1957) Purified preparations of this enzyme will contain some, if not all, of the components of the sodium pump.

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