Characterization of the translocated chromosome using fluorescencein situ hybridization and random amplified polymorphic DNA on twoTriticum aestivum—Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus

1996 ◽  
Vol 4 (8) ◽  
pp. 583-587 ◽  
Author(s):  
Richard R. -C. Wang ◽  
Xue-Yong Zhang
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Random Amplified Polymorphic Dna ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Fluorescencein Situ Hybridization ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Translocation Lines ◽  
Yellow Dwarf Virus

Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 698-706 ◽  
Author(s):  
O R Crasta ◽  
M G Francki ◽  
D B Bucholtz ◽  
H C Sharma ◽  
J Zhang ◽  
...  
Keyword(s):  
Repetitive Sequence ◽  
Barley Yellow Dwarf Virus ◽  
Rflp Analysis ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Translocation Lines ◽  
Yellow Dwarf Virus ◽  
Alien Chromatin

Stable introgression of agronomically important traits into crop plants through wide crossing often requires the generation and identification of translocation lines. However, the low efficiency of identifying lines containing translocations is a significant limitation in utilizing valuable alien chromatin-derived traits. Selection of putative wheatgrass-wheat translocation lines based on segregation ratios of progeny from γ-irradiated seed using a standard phenotypic analysis resulted in a low 4% success rate of identifying barley yellow dwarf virus (BYDV) resistant and susceptible translocation lines. However, 58% of the susceptible progeny of this irradiated seed contained a Thinopyrum intermedium chromosome-specific repetitive sequence, which indicated that γ-irradiation-induced translocations occurred at high rate. Restriction fragment length polymorphism (RFLP) analysis of susceptible lines containing alien chromatin, their resistant sister lines and other resistant lines showed that more than one third of the progeny of γ-irradiated double monosomic seeds contained wheatgrass-wheat translocations. Genomic in situ hybridization (GISH) analysis of selected lines confirmed that these were wheatgrass-wheat translocation lines. This approach of initially identifying BYDV susceptible deletion lines using an alien chromosome-specific repetitive sequence followed by RFLP analysis of their resistant sister lines efficiently identified resistant translocation lines and localized the BYDV resistance to the distal end of the introgressed Th. intermedium chromosome.Key words: gene introgression, wide crosses, chromosome, repetitive elements, RFLP, Thinopyrum intermedium.

Characterization of a potential source of barley yellow dwarf virus resistance for wheat

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 250-257 ◽  
Author(s):  
Z. Y. Xin ◽  
R. I. S. Brettell ◽  
Z. M. Cheng ◽  
P. M. Waterhouse ◽  
R. Appels ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Virus Resistance ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Potential Source ◽  
Yellow Dwarf Virus

Resistance to barley yellow dwarf virus (BYDV) has been described in lines derived from crosses between wheat and Thinopyrum intermedium. The resistance in one such line, Zhong 4, originating in China, was expressed against two different serotypes of BYDV as measured by accumulation of virus in infected seedlings. F1 hybrids between wheat (2n = 42) and Zhong 4 (2n = 56) had 49 chromosomes and intermediate levels of virus when compared to the parent lines. Cytological and molecular hybridization studies suggested that the BYDV resistance in Zhong 4 is carried on a set of seven pairs of nonwheat chromosomes that derived from a combination of the E and X genomes present in Th. intermedium.Key words: Barley yellow dwarf virus, wheat, ribosomal DNA, Agropyron intermedium, Thinopyrum intermedium, virus resistance.

scholarly journals Molecular characterization of barley yellow dwarf virus in Tunisia

2014 ◽  
Vol 58 (03) ◽  
pp. 214-222 ◽  
Author(s):  
M. BOUALLEGUE ◽  
M. MEZGHANI-KHEMAKHEM ◽  
D. BOUKTILA ◽  
H. MAKNI ◽  
M. MAKNI
Keyword(s):  
Molecular Characterization ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus
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