Comparison of the thin layer chromatography/flame ionization detection system with other methods for the quantitative analysis of liver lipid contents in alcohol-fed rats and controls

Lipids ◽  
1985 ◽  
Vol 20 (8) ◽  
pp. 531-535 ◽  
Author(s):  
G. Ananda Rao ◽  
Diana E. Riley ◽  
Edward C. Larkin
1988 ◽  
Vol 58 (10) ◽  
pp. 593-600 ◽  
Author(s):  
Ian H. Leaver ◽  
David M. Lewis ◽  
David J. Westmoreland

This paper examines the usefulness of thin-layer chromatography (TLC), coupled with an automated quantitative detection system based on flame ionization detection (FID), for the qualitative and quantitative determination of lipids in wool. The latroscan TLC-FID system has been used to determine the composition of the solvent soluble material (internal lipids) isolated from wool after Soxhlet extraction with a chloroform/methanol azeotrope, and to investigate whether scouring treatments affect the composition of the internal lipids. Changes in the composition of wool grease that occur as a result of exposure to sunlight (behind glass) and during weathering in the fleece are also examined.


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