Cloning and characterization of NBS-LRR class resistance-gene candidate sequences in citrus

2000 ◽  
Vol 101 (5-6) ◽  
pp. 814-822 ◽  
Author(s):  
Z. Deng ◽  
S. Huang ◽  
P. Ling ◽  
C. Chen ◽  
C. Yu ◽  
...  
2018 ◽  
Vol 44 (4) ◽  
pp. 473 ◽  
Author(s):  
Zhan-Wang ZHU ◽  
Deng-An XU ◽  
Shun-He CHENG ◽  
Chun-Bao GAO ◽  
Xian-Chun XIA ◽  
...  

1991 ◽  
Vol 55 (7) ◽  
pp. 1913-1918
Author(s):  
Ryutaro Utsumi ◽  
Tadashi Yagi ◽  
Satoshi Katayama ◽  
Kiyonori Katsuragi ◽  
Kouji Tachibana ◽  
...  

Plant Science ◽  
2003 ◽  
Vol 164 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Maria Rosaria Cortese ◽  
Elena Fanelli ◽  
Carla De Giorgi

1997 ◽  
Vol 41 (2) ◽  
pp. 314-318 ◽  
Author(s):  
E Hannecart-Pokorni ◽  
F Depuydt ◽  
L de wit ◽  
E van Bossuyt ◽  
J Content ◽  
...  

The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.


1999 ◽  
Vol 43 (12) ◽  
pp. 2925-2929 ◽  
Author(s):  
Lydia Bass ◽  
Cynthia A. Liebert ◽  
Margie D. Lee ◽  
Anne O. Summers ◽  
David G. White ◽  
...  

ABSTRACT Antibiotic resistance among avian bacterial isolates is common and is of great concern to the poultry industry. Approximately 36% (n = 100) of avian, pathogenic Escherichia coli isolates obtained from diseased poultry exhibited multiple-antibiotic resistance to tetracycline, oxytetracycline, streptomycin, sulfonamides, and gentamicin. Clinical avian E. coli isolates were further screened for the presence of markers for class 1 integrons, the integron recombinase intI1 and the quaternary ammonium resistance gene qacEΔ1, in order to determine the contribution of integrons to the observed multiple-antibiotic resistance phenotypes. Sixty-three percent of the clinical isolates were positive for the class 1 integron markersintI1 and qacEΔ1. PCR analysis with the conserved class 1 integron primers yielded amplicons of approximately 1 kb from E. coli isolates positive for intI1 andqacEΔ1. These PCR amplicons contained the spectinomycin-streptomycin resistance gene aadA1. Further characterization of the identified integrons revealed that many were part of the transposon Tn21, a genetic element that encodes both antibiotic resistance and heavy-metal resistance to mercuric compounds. Fifty percent of the clinical isolates positive for the integron marker gene intI1 as well as for theqacEΔ1 and aadA1 cassettes also contained the mercury reductase gene merA. The correlation between the presence of the merA gene with that of the integrase and antibiotic resistance genes suggests that these integrons are located in Tn21. The presence of these elements among avianE. coli isolates of diverse genetic makeup as well as inSalmonella suggests the mobility of Tn21 among pathogens in humans as well as poultry.


2019 ◽  
Vol 117 (4) ◽  
pp. 617
Author(s):  
Anand C. Reddy ◽  
B. Lavanya ◽  
T. Tejaswi ◽  
E. Sreenivasa Rao ◽  
D. C. Lakshmana Reddy

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