Surface display of active lipases Lip7 and Lip8 from Yarrowia Lipolytica on Saccharomyces Cerevisiae

2010 ◽  
Vol 88 (4) ◽  
pp. 885-891 ◽  
Author(s):  
Wen-Shan Liu ◽  
Xiao-Xing Pan ◽  
Bin Jia ◽  
He-Yun Zhao ◽  
Li Xu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yarrowia Lipolytica ◽  
Surface Display

scholarly journals Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

Catalysts ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 757
Author(s):  
Huiyi Shang ◽  
Danni Yang ◽  
Dairong Qiao ◽  
Hui Xu ◽  
Yi Cao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Large Scale ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Fusion Partner ◽  
Whole Cell ◽  
Food Industries ◽  
Yeast Surface ◽  
The Stability

Levan has wide applications in chemical, cosmetic, pharmaceutical and food industries. The free levansucrase is usually used in the biosynthesis of levan, but the poor reusability and low stability of free levansucrase have limited its large-scale use. To address this problem, the surface-displayed levansucrase in Saccharomyces cerevisiae were generated and evaluated in this study. The levansucrase from Zymomonas mobilis was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a various yeast surface display platform. The N-terminal fusion partner is based on a-agglutinin, and the C-terminal one is Flo1p. The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively. Meanwhile, the stability of the surface-displayed levansucrases is significantly enhanced. After six reuses, these two biocatalysts retained over 50% and 60% of their initial activities, respectively. Furthermore, the molecular weight and polydispersity test of the products suggested that the whole-cell biocatalyst of levansucrase displayed by Flo1p has more potentials in the production of levan with low molecular weight which is critical in certain applications. In conclusion, our method not only enable the possibility to reuse the enzyme, but also improves the stability of the enzyme.

The Surface Display of the Alginate Lyase on the Cells of Yarrowia lipolytica for Hydrolysis of Alginate

2009 ◽  
Vol 11 (5) ◽  
pp. 619-626 ◽  
Author(s):  
Guanglei Liu ◽  
Lixi Yue ◽  
Zhe Chi ◽  
Wengong Yu ◽  
Zhenming Chi ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Alginate Lyase ◽  
Hydrolysis Of

Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

2019 ◽  
Vol 21 (7) ◽  
pp. 1795-1808 ◽  
Author(s):  
Gregory Guirimand ◽  
Kentaro Inokuma ◽  
Takahiro Bamba ◽  
Mami Matsuda ◽  
Kenta Morita ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Cell Surface ◽  
Surface Display ◽  
Woody Biomass ◽  
Cell Surface Display ◽  
Xylitol Production ◽  
Display Technology ◽  
Pharmaceutical Industries

Xylitol is a major commodity chemical widely used in both the food and pharmaceutical industries.

scholarly journals Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Xiaoyu Yang ◽  
Hongting Tang ◽  
Meihui Song ◽  
Yu Shen ◽  
Jin Hou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Surface Display ◽  
Heterologous Proteins

Surface display of acid protease on the cells of Yarrowia lipolytica for milk clotting

2010 ◽  
Vol 87 (2) ◽  
pp. 669-677 ◽  
Author(s):  
Xin-Jun Yu ◽  
Catherine Madzak ◽  
Hui-Juan Li ◽  
Zhen-Ming Chi ◽  
Jing Li
Keyword(s):  
Yarrowia Lipolytica ◽  
Surface Display ◽  
Acid Protease ◽  
Milk Clotting
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