Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst

2011 ◽  
Vol 90 (5) ◽  
pp. 1731-1738 ◽  
Author(s):  
Shinji Hama ◽  
Kazunori Miura ◽  
Ayumi Yoshida ◽  
Hideo Noda ◽  
Hideki Fukuda ◽  
...  
Keyword(s):  
Rhizopus Oryzae ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Direct Use

scholarly journals Immobilization of Rhizopus oryzae LY6 onto Loofah Sponge as a Whole-Cell Biocatalyst for Biodiesel Production

BioResources ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 850-860 ◽  
Author(s):  
Qiyang He ◽  
Hao Shi ◽  
Huaxiang Gu ◽  
Gilda Naka ◽  
Huaihai Ding ◽  
...  
Keyword(s):  
Rhizopus Oryzae ◽  
Methyl Esters ◽  
Cell Immobilization ◽  
Fold Increase ◽  
Biodiesel Production ◽  
Whole Cell ◽  
Loofah Sponge ◽  
Whole Cell Biocatalyst ◽  
Optimized Conditions ◽  
Significant Attention

Whole cell biocatalysts for biodiesel production have garnered significant attention in recent years, as they can help avoid the complex procedures of isolation, purification, and immobilization of extracellular lipase. Because of its renewability and biodegradability, loofah (Luffa cylindrica) sponge is an advantageous substitute for traditional biomass carriers in whole cell immobilization. Rhizopus oryzae mycelia can spontaneously attach onto loofah sponge particles (LSPs) during cell cultivation. The highest immobilized R. oryzae cells concentration can reach up to 1.40 g/1 g of LSPs. The effects of biocatalyst addition and water content on methanolysis for biodiesel production were investigated in this paper. The operational stability of glutaraldehyde-treated biocatalyst at 35 °C, using a 1:1 oil-to-methanol ratio, was assayed, revealing a 3.4-fold increase in half-life compared with the untreated biocatalyst. Under optimized conditions, the yield of methyl esters in the reaction mixture reached 82.2% to 92.2% in each cycle. These results suggested that loofah sponge is a potential fungi carrier for an immobilized whole-cell biocatalyst.

Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil)

2012 ◽  
Vol 32 (8) ◽  
pp. 1539-1547 ◽  
Author(s):  
Bharathiraja Balasubramaniam ◽  
Ayyappasamy Sudalaiyadum Perumal ◽  
Jayamuthunagai Jayaraman ◽  
Jayakumar Mani ◽  
Praveenkumar Ramanujam
Keyword(s):  
Fatty Acid ◽  
Comparative Analysis ◽  
Sunflower Oil ◽  
Rhizopus Oryzae ◽  
Waste Cooking Oil ◽  
Whole Cell ◽  
Cooking Oil ◽  
Whole Cell Biocatalyst ◽  
Oil Sunflower

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst

Production of biodiesel by immobilized whole-cell biocatalyst

2008 ◽  
Vol 136 ◽  
pp. S385-S386
Author(s):  
Jyh-Ping Chen ◽  
Shu-Chin Chang
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst
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