In vitro evaluation of the growth inhibition and apoptosis effect of mifepristone (RU486) in human Ishikawa and HEC1A endometrial cancer cell lines

2007 ◽  
Vol 62 (3) ◽  
pp. 483-489 ◽  
Author(s):  
Marisa A. Navo ◽  
Judith A. Smith ◽  
Anjali Gaikwad ◽  
Thomas Burke ◽  
Jubilee Brown ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Endometrial Cancer Cell ◽  
In Vitro Evaluation

Variances in the Expression Profile of the EMT-Related Genes in Endometrial Cancer Lines In Vitro Study

2021 ◽  
Vol 22 ◽  
Author(s):  
Robert Nowakowski ◽  
Beniamin Grabarek ◽  
Anna Burnat-Olech ◽  
Dariusz Boroń ◽  
Monika Paul-Samojedny
Keyword(s):  
Endometrial Cancer ◽  
Expression Pattern ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Cultures ◽  
Expression Profile ◽  
Histological Grade ◽  
Cancer Cell Lines ◽  
Endometrial Cancer Cell

Background: This study aimed to evaluate the variances in the expression pattern of mRNAs and miRNAs related to the EMT in the Ishikawa (histological grade 1; G1), EC-1A (histological grade 2; G2), and KLE (histological grade 3; G3) cell cultures under cisplatin treatment. Methods: Endometrial cancer cell lines were exposed to 75.22 mg (an average concentration of the drug used in patients with endometrial cancer) for 12.24 and 48 hours compared to the untreated cells (control). The molecular analysis included extraction of total RNA, microarray analysis (mRNA and miRNA), RTqPCR, and the ELISA assay. Results: Out of 226 mRNAs associated with the EMT, the number of mRNAs differentially expressed in endometrial cancer cell cultures treated with cisplatin compared to a control culture was as follows: Ishikawa line - 87 mRNAs; EC-1A - 84 mRNAs; KLE - 71 mRNAs (p<0.05). The greatest changes in the Ishikawa line treated with the drug compared to the control were noticed for mRNA STAT1 TGFβ1, SMAD3, FOXO8, whereas in EC-1A, they were mRNA TGFβ1, BAMBI, SMAD4, and in KLE mRNA COL1A1, FOXO8, TGFβ1. The analysis also showed that miR-106a, miR-30d, miR-300 are common for all cell lines used in this experiment. Conclusion: Cisplatin changes the expression profile of genes associated with EMT in endometrial cancer cell lines. It seems that the expression pattern of TGFβ1 might be a promising, supplementary molecular marker of the effectiveness of cisplatin therapy. The analysis showed that miR-30d, miR-300, and miR-106a are involved in the regulation of the expression of EMT-related genes.

p53 Interference and Growth Inhibition in p53-Mutant and Overexpressing Endometrial Cancer Cell Lines

1997 ◽  
Vol 66 (1) ◽  
pp. 94-102 ◽  
Author(s):  
Mike F. Janicek ◽  
Roberto Angioli ◽  
Ayçe D. Unal ◽  
Bernd-Uwe Sevin ◽  
Marilu Madrigal ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Endometrial Cancer Cell
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