Identification of Major Enzymes Involved in the Synthesis of Diadenosine Tetraphosphate and/or Adenosine Tetraphosphate in Myxococcus xanthus

2018 ◽  
Vol 75 (7) ◽  
pp. 811-817 ◽  
Author(s):  
Yoshio Kimura ◽  
Chihiro Tanaka ◽  
Manami Oka
Keyword(s):  
Myxococcus Xanthus ◽  
Diadenosine Tetraphosphate

Preparation of Outer Membrane Vesicles from Myxococcus xanthus

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
James Berleman ◽  
Marcin Zemla ◽  
Jonathan Remis ◽  
Manfred Auer
Keyword(s):  
Outer Membrane ◽  
Myxococcus Xanthus ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

scholarly journals Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shukria Akbar ◽  
D. Cole Stevens
Keyword(s):  
Pseudomonas Putida ◽  
Bacterial Communities ◽  
Predator Avoidance ◽  
Phenotypic Diversity ◽  
Myxococcus Xanthus ◽  
Predator Prey ◽  
Mucoid Conversion ◽  
Small Colony ◽  
Predator Prey Models ◽  
Insight Into

AbstractPredation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predatory mechanisms and features that facilitate consumption of prey. However, prey resistance to myxobacterial predation remains underexplored, and prey resistances have been observed exclusively from predator–prey experiments that included the model myxobacterium Myxococcus xanthus. Utilizing a predator–prey pairing that instead included the myxobacterium, Cystobacter ferrugineus, with Pseudomonas putida as prey, we observed surviving phenotypes capable of eluding predation. Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor phenotype suggested that increased expression of efflux pumps, genes associated with mucoid conversion, and various membrane features contribute to predator avoidance. Unique features observed from the survivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediated antibiotic resistance, phenazine-1-carboxylic acid production, and increased mucoid conversion. These results demonstrate the utility of myxobacterial predator–prey models and provide insight into prey resistances in response to predatory stress that might contribute to the phenotypic diversity and structure of bacterial communities.

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